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A: DNA ligase plays an important role in recombinant DNA technology.
R: The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.
A: Restriction enzymes belong to a larger class of enzymes called nucleases.
R: Each restriction enzyme recognises a specific palindromic nucleotide sequence in the DNA.
A: During gel electrophoresis, the DNA fragments move towards the anode.
R: DNA fragments are negatively charged molecules.
A: Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure.
R: It requires simultaneous plating on two plates having different antibiotics.
A: Taq Polymerase is involved in PCR technique.
R: This enzyme remains active during the high temperature, including denaturation of double stranded DNA.
A: Myocardial infarction can now be treated more effectively due to genetic engineering.
R: Streptokinase produced by Streptococcus and modified by RDT is used as a clot buster for removing thrombus.
A: Disarmed Ti plasmid can be utilised as a shuttle vector.
R: It can survive in both prokaryotic and eukaryotic organisms.
A: Pst I generates single stranded overhanging stretches in DNA after digestion that facilitate ligation.
R: These short extensions can form phosphodiester bonds with their complementary counterparts.
A: Ethidium bromide helps in visualizing DNA in UV light only.
R: This intercalating agent absorbs light in range 260 – 330 nm.
A: To extract RNA from tomato cells, first it must be treated with cellulase, protease and then deoxyribonuclease.
R: Deoxyribonuclease will digest DNA while RNA will be intact.