Biotechnology - 2014 Past Year Paper


100 Questions MCQ Test IIT JAM Past Year Papers and Model Test Paper (All Branches) | Biotechnology - 2014 Past Year Paper


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QUESTION: 1

Match the proteins listed in Column- I with their major cellular function in Column- II:

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QUESTION: 2

Amongst the following, the elongated, fibrous protein is

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QUESTION: 3

The mutation likely to cause the least perturbation in the tertiary structure of a protein is

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QUESTION: 4

Match the techniques in Column- I with their primary applications in Column- II:

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QUESTION: 5

Amongst the following statements about biological membranes, the INCORRECT one is that they

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QUESTION: 6

The introduction of a new fish species into a lake resulting in the extinction of several native fish species from the lake is an example of

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QUESTION: 7

The taxonomic hierarchy in descending order of size is

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QUESTION: 8

If the recessive disease phenylketonuria (PKU) occurs in a genetically constant population with a frequency of 1 in 10000, the frequency of the carrier genotype is

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QUESTION: 9

Amplification of a DNA fragment by PCR yields only one faint band of the expected size on an agarose gel. For such a sample, the best way to increase the yield of the PCR product is to

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QUESTION: 10

The cellular organelle which function(s) as a store for Ca2+ ion is

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QUESTION: 11

If the N- terminal 21 amino acids were missing from a mitochondrial protein, its cellular location after synthesis would be

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QUESTION: 12

Packaged biomaterials are dispatched to intracellular and extracellular locations from the

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QUESTION: 13

The preferred ligand for SH2 domain is

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QUESTION: 14

The binding of a hormone to its receptor activates adenylyl cyclase through a stimulatory G protein. If, due to a mutation, the G- protein binds but does NOT hydrolyze GTP, the consequence will be

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QUESTION: 15

A toxin which causes accumulation of twice the normal amount of DNA in a dividing mammalian cell, most likely blocks the cell cycle

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QUESTION: 16

Bt toxin, produced by Bacillus thuringiensis, does NOT kill the bacteria itself because the toxin is

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QUESTION: 17

The inactivation of an mRNA due to its binding to a complementary RNA molecule is called

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QUESTION: 18

Given are the sequences of one strand of double-stranded DNA. The one with the highest melting point (Tm) is

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QUESTION: 19

The standard pregnancy kit, used to detect Human Chorionic Gonadotrophin (HCG) in urine, is based on

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QUESTION: 20

The preferred system of large- scale production of influenze virus for vaccination is

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QUESTION: 21

A monoclonal antibody produced against a small peptide derived from protein X, is unable to bind X in an ELISA. This is because

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QUESTION: 22

The lac repressor is produced from a stretch of DNA called the

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QUESTION: 23

The repeating units in chitin are

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QUESTION: 24

The correct ascending order of melting points of oleic acid (O), linoleic acid (L), palmitic acid (P) and stearic acid (L), palmitic acid (P) and stearic acid (S) is

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QUESTION: 25

A peptide Glu- His- Trp- Ser- Gly- Leu- Arg- Pro- Gly, having an isoelectric point of 7.8, is placed in an electric field at pH 3.0. It will migrate towards

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QUESTION: 26

X- ray diffraction of wool shows repeated structural units speed at 5.2 Å, which is changed to 7.0 Å on steaming. This is due to the conversion of secondary structure from

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QUESTION: 27

At Et = 20 nm and substrate concentration = 40 mM, the reaction velocity V0 of an enzyme is 9.6 mM s-1. Assuming kcat to be 600 s-1, the KM will be

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QUESTION: 28

Which of the following statements is NOT true for an enzyme catalyzed reaction?

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QUESTION: 29

Which of the following is NOT an allosteric regulatory enzyme in glycolysis?

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QUESTION: 30

Match the enzymes of TCA cycle in Group- I with that of their products listed in Group- II

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QUESTION: 31

Addition of the uncoupler 2, 4- Dinitrophenol to actively respiring mitochondria causes

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QUESTION: 32

C4 plants overcome photorespiration activity of Rubisco by fixing CO2, firstly as

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QUESTION: 33

Which of the following is a non- symbiotic nitrogen fixing bacteria?

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QUESTION: 34

Vasopressin, an antidiuretic hormone, responsible for increased absorption of water by the kidney, is secreted from

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QUESTION: 35

Match the vitamins in Group- I with their deficiency disorders listed in Group- II.

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QUESTION: 36

The protein content called novel is produced by using

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QUESTION: 37

Which part of the human brain controls body temperature?

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QUESTION: 38

The action potential for initiating and maintaining the rhythmic contraction of heart is generated by

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QUESTION: 39

The antigen binding sites in immunoglobulin IgG are present at

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QUESTION: 40

Fertilization of human sperm and ovum takes place in the

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QUESTION: 41

Match the pathogenic microorganisms in Group- I with the diseases listed in Group- II

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QUESTION: 42

An example of a eukaryotic chemoorganotroph microorganism lacking chlorophyll and having mycelial thallus is

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QUESTION: 43

A bacillus sp. divides every 30 min. If a culture is inoculated with 1000 cells, how many cells will be generated after 3 hrs?

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QUESTION: 44

The selective media mannitol salt agar is used for the isolation of

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QUESTION: 45

In liver cells, glucose is converted to glucose- 6- P which can then be utilized towards glycolysis or glycogen synthesis. If KMGlycolysis and KMGlycogen correspond to the enzymes involved in the first steps of glycolysis and glycogen synthesis, the true statement amongst the following is:

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QUESTION: 46

An enzyme requires both aspartate (pKa of side chain = 4.5) and histidine (pKa of side chain = 6.5) residues in the catalytic site to be protonated for activity. The expected enzyme activity (in %) at a pH of 5.5 would be closed to

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QUESTION: 47

By weight, 95% of an E. coli cell’s components are water (~70%), protein (~15%), nucleic acids (DNA ~ 1% + RNA ~ 6%) and polysaccharides (~3%). Given that thee is only one chromosome and about 3000 different proteins in an E. coli cell lysate, the number of different molecules of DNA and RNA is expected to be

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QUESTION: 48

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion : The general trend across a period is an increase of the ionization energy.
Reason : The potential energy of attraction between the electron and nucleus increases with the nuclear charge.

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QUESTION: 49

The monomer which leads to a conducting polymer is

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QUESTION: 50

The pH at the equivalence point when 50 mL of 0.1 M acetic acid is titrated against 0.1 M NaOH is closes to

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QUESTION: 51

The mass (in g) of glycine, NH2CH2COOH, required to make 250 mL of a 0.015 M solution is (Atomic weights in amu: H = 1, C = 12, N = 14, O = 6)

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QUESTION: 52

The arrangement of ligands in ascending order of the crystal field splitting is

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QUESTION: 53

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion : The boiling points of the group VIA (16) hydrides increase with size without exception.
Reason : London dispersion forces increase with molecular weight.

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QUESTION: 54

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion : Boiling points of aldehydes and ketones are higher than the boiling points of the corresponding ethers and lower than alcohols.
Reason : The carbonyl group is polar but does not undergo intermolecular hydrogen bonding.

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QUESTION: 55

In the reaction sequence below, X, Y, and Z, respectively, are

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QUESTION: 56

On completion of the reaction the Br atom is attached to carbon atom

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QUESTION: 57

An aqueous solution is a mixture of a carboxylic acid (pKa = 4.0) and an amine (pKa of protonated amine = 10.0). To separate the components, the solution at a pH of 2.0 is shaken with diethyl either. On standing, the

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QUESTION: 58

The major product, Z, obtained in the reaction

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QUESTION: 59

The compound that shows a line in the 1H NMR spectrum at the lowest d value is

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QUESTION: 60

Water is injected into a balloon filled with ammonia gas. The balloon shrinks and it is hot to touch.According to the convention ΔU = q + w, for this process

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QUESTION: 61

A process CANNOT occur spontaneously at constant T and P when

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QUESTION: 62

If an atomic orbital has 2 radial nodes and 1 angular node, it is a

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QUESTION: 63

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion : Water at 100°C and 1 atm is acidic with a pH less than 7.
Reason : The ionic product of water, Kw, decreases when T increases because the enthalpy of the dissociation of water is endothermic.

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QUESTION: 64

A mixture initially containing 2 mol of CO and 2 mol of H2 comes to equilibrium with methanol, CH3OH, as the product of the reaction CO (g) + 2H2(g) → CH3OH (g). At equilibrium the mixture will contain

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QUESTION: 65

Given that the standard electrode potentials E° (Cu2+ | Cu) = +0.340 V and E° (Cu+ | Cu) = +0.522 V, the E° (Cu2+ | Cu+) is

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QUESTION: 66

The number of water molecules required to balance the chemical reaction when MnO4 is converted to MnO2 in basic solution is

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QUESTION: 67

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QUESTION: 68

Match the type of transition in the left column with the frequency of the electromagnetic radiation in the right column.

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QUESTION: 69

The postulates of Bohr’s theory of the atom are I. Electrons move in stable circular orbits around the nucleus.
II. Electrons may absorbs light of specific energy and be excited to higher energy states.
III. Angular momentum of electrons in stable orbits is quantized.
IV. Angular momentum of electrons in stable orbits is uncertain.

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QUESTION: 70

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion : Blood pressure in humans is greater at the brain than at the feet.
Reason : Human heart is father from the feet than the brain.

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QUESTION: 71

A jellyfish appearing translucent in the sea disappears when immersed in an aquarium having liquid X. If the refractive index of the jellyfish is n, refractive index of X is

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QUESTION: 72

The dimension of shear strain are

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QUESTION: 73

In S.I. units, electric flux, which is the dot product of electric field intensity and area vector, are

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QUESTION: 74

The zeroth law of thermodynamics

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QUESTION: 75

If a human heart beats at an average frequency of 1.25 Hz, the number of beats per minute is

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QUESTION: 76

For a mammalian skeletal muscle, if the extracellular potassium ion concentration, [K+]out = 4 mM, and, the intracellular potassium ion concentration, [K+]in = 128 mM, the approximate potassium ion (K+) potential (in mV) is Assume : Faraday’s constant = 9.65 × 104 C mol–1; Gas constant = 8.31 J K–1 mol–1; mammalian temperature = 37°C

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QUESTION: 77

Optical resolution of a light microscope is limited by

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QUESTION: 78

Aqueous environment in a spherical endosome—a closed vesicle of 100 nm diameter, is at a pH 5.0, in order to denature and hydrolyze the material internalized by a cell. Assuming Avogadro’s number to be 6 × 1023, the number of free protons in an endosome is closed to

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QUESTION: 79

Newton’s second law of motion deals with

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QUESTION: 80

If the work done by a human heart is 0.5 J per beat, with the time period “T” of 0.8 seconds for a beat, the approximate energy (in kcal) required by a human heart to beat to beat in a day is Assume: 1 calorie = 4.2 Joules

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QUESTION: 81

The maximum depth (in m) reachable by a research submarine, designed to withstand an external pressure of 100 atm, is Assume: Density of water = 1000 kg/m3, acceleration due to gravity = 10 m/s2, and, atmospheric pressure at the water surface = 1 atm = 105 Pa

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QUESTION: 82

The number of water molecules present in a 300 residue soluble protein of spherical shape (diameter = 2 nm) having 20% (V/V) water is closed to Assume: density of water = 1000 kg/m3, Avogadro’s number = 6 × 1023.

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QUESTION: 83

If the four bases, A, T, G, and C, occur with equal likelihood in a five nucleotide DNA sequence, the approximate probability of finding the sequence CGAGT through random chance is

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QUESTION: 84

A new life form has DNA with 5 nucleotides instead of 4, 5 naturally occurring amino acids instead of 20, and a codon size of 2 bases instead of 3, Asswning the central dogma of biology applies to the new life form, the degeneracy of its genetic code

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QUESTION: 85

20 microbial colonies appear on an agar plate having sources and cellulose as the only carbon sources. Of the 20 colonies, microbes in 12 colonies can metabolize source. If microbes in 4 colonies metabolize both source and cellulose, the number of colonies with microbes which can metabolize cellulose is

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QUESTION: 86

A heavy chain of an immunoglobulin is a result of recombination of one DNA segment each from 200 different V segments, 12 D segments and 4 J segments from the corresponding DNA sequence. Further, a light chain results from recombination of one segment each from 200 different V segments with 5 different J segments. If a heavy chain and a light chain form an immunoglobulin, the maximum number of different immunoglobulins that can be synthesized is closed to

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QUESTION: 87

Weight of a colony of a newly discovered unicellular organism can be predicted by the empirical equation W (in g) = (xn), where x = 1.01 and n = number of cells in the colony. If a dog weight 10 kg, the correct statement amongst the following is

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QUESTION: 88

Given that a hetero- trimeric protein is formed by 3 proteins with their center of masses at the coordinates (1, 1, 2), (3, –5, 7) and (–1, 7, –6) respectively, the coordinates of the centroid of the triangle formed by joining the three center of masses is

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QUESTION: 89

Two linear and parallel RNA strands, defined by the equations 3x – 4y + 6 = 0 and 3x – 4y + 5 = 0 are hydrogen bonded together. The distance between the two strands is

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QUESTION: 90

(p, q + r), (q, r + p), and (r, p + q) are the coordinates of 3 co- planar atoms in a molecular structure. Area occupied by the three atoms is

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QUESTION: 91

The angle between two linear transmembrane domains defined by the following vectors

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QUESTION: 92

A straight DNA segment defined by 6ˆi + 2j - 8k is bound to a linear transcription factor defined by 4i - 4j + 2k . The correct statement amongst the following is

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QUESTION: 93

A protein backbone can be traced by plotting the coordinates of Cα atoms of its constituent amino acids. If area of the triangle formed by joining the coordinates of Cα atoms of its constituent amino acids. If area of the triangle formed by joining the coordinates of Cα atoms of a tripeptide is zero,

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QUESTION: 94

Priyanka has blood type O. Her mother’s blood type is B and father’s blood type is A. If both of Priyanka’s grandmothers had blood type AB, and assuming NO child was adopted, blood type of either of her grandfathers CANNOT be

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QUESTION: 95

Given that you have 2 parents, 4 grandparents, 8 greater grand parents, and so on, the number of your ancestors during ten (10) generations of your family preceding you is

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QUESTION: 96

The intracellular non- enzymetic fractional degradation of a compound X, f(x), is related to its concentration x through

At a negligible concentration of X, its fractional degradation is

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QUESTION: 97

The distance x (in mm) covered by a molecule starting from point A at time t = 0 and stopping at another point B is given by the equation

The distance between A and B (in mm) is closed to

Solution:
QUESTION: 98

Dependence of the weight, y (in kg), of an organism on the number of hours, x when it is in motion, is given by the differential equation

Given that y = 1, when x = 0, the weight of the organism after moving for one hour is

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QUESTION: 99

A hospital has 35 patients, 24 of which are HIV + and 16 have TB infection. All patients have at least one of the two infections. The number of patients with both HIV and TB infections is

Solution:
QUESTION: 100

The average weight of four kids is 29.6 kg. If three of the kids weigh 29.8 kg, 28.6 kg and 29.7 kg respectively, the weight of the fourth kid (in kg) is

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