Biotechnology Engineering - BT 2017 GATE Paper MCQ Quiz - GATE MCQ

The transcription factor X binds a 10 base pair DNA stretch. In the DNA of an organism, X was found to bind at 20 distinct sites. An analysis of these 20 binding sites showed the following distribution: 

Q. What is the consensus sequence for the binding site of X? 

Macrophages eliminate pathogenic bacteria upon activation by  

A bacterium has a genome of size 6 million base pairs. If the average rate of DNA synthesis is 1000 base paris/second, the time taken (in minutes) for replication of the genome will be ___

An enzyme catalyzes a reaction by  

Which one of the following techniques can be used to determine the structure of a 15 kDa globular protein at atomic resolution?  

In eukaryotes, cytokinesis is inhibited by  

Which one of the following mechanisms is used by human pathogens to evade host immune responses?  

An enzyme reaction exhibits Michaelis-Menten kinetics. For this reaction, on doubling the concentration of enzyme while maintaining [S] >> [E₀].  

The surface area (in m²) of the largest sphere that can fit into a hollow cube with edges of length 1 meter is ______ .

Given data: π = 3.14

Assertion [a]: Gram negative bacteria show staining with saffranin.

Reason [r] : Gram negative bacteria have an outer membrane with lipopolysaccharides  

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?  

For y f (x), if  = 0 at x = 0, and y = 1 at x = 1, the value of y at x = 2 is ____ . 

If a protein contains four cysteine residues, the number of different ways they can simultaneously form two intra-molecular disulphide bonds is ____. 

Natural proteins are composed primarily of 20 α − amino acids. Which one of the following statements is true for any of these amino acids in a solution of p^H 1.5?  

During protein synthesis, tRNAs are NOT involved in  

A polymerase chain reaction (PCR) was set up with the following reagents: DNA template. Taq polymerase, butter, dNTPs, and Mg²⁺ . Which one of the following is missing in the reaction mixture?  

The plant hormone indole-3-acetic acid is derived from  

Secretory proteins synthesized by ER-associated ribosomes traverse through  

A 5 liter chemostat is fed fresh medium at 0.2 litres/minute having a substrate concentration of 25 grams/liter. At steady state, the outgoing stream has substrate concentration of 2.5 grams/liter. The rate of consumption (grams/liter/minute) of the substrate in the reactor is ___. 

If the nucleotide composition (%) of a viral genome is A = 10, U = 20, C = 40, and G = 30,

which one of the following is this genome?  

In a thin layer chromatography experiment using a silica gel plate, a compound showed migration of 12.5 cm and the solvent front showed migration of 18 cm. The Rf value for the compound is ____ . 

A pedigree of an inheritable disease is shown below.

This inheritable disease is

A DNA strand of length 25 mm wraps diametrically around the circumference of a spherical histone-octamer once. The radius (nm) of the histone-octamer is _____ .

 Given data: π = 3.14

A recombinant protein is to be expressed under the control of the lac promoter and operator in a strain of E.coli having the genotype lacl⁺ crp⁺. Even in the absence of inducer IPTG, low levels of expression of the recombinant protein are seen (leaky expression). Which one of the following should be done to minimize such leaky expression?  

EcoRI restriction sites on a 10kb DNA fragment are shown below. 

Upon partial digestion, what are the lengths (in kb) of all the possible DNA fragments obtained?

A zero-order liquid phase reaction  is being carried out in a batch reactor with k = 10³ mol / min. If the starting concentration of A is 0.1 moles/liter, the time (in minutes) taken by the system before A is exhausted in a 100 liter reactor is ____ .