Microbiology MCQ 2


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21 Questions MCQ Test Mock Test Series of IIT JAM Biotechnology | Microbiology MCQ 2

Microbiology MCQ 2 for IIT JAM 2022 is part of Mock Test Series of IIT JAM Biotechnology preparation. The Microbiology MCQ 2 questions and answers have been prepared according to the IIT JAM exam syllabus.The Microbiology MCQ 2 MCQs are made for IIT JAM 2022 Exam. Find important definitions, questions, notes, meanings, examples, exercises, MCQs and online tests for Microbiology MCQ 2 below.
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Microbiology MCQ 2 - Question 1

Acid fast staining targets which of the following compound? 

Detailed Solution for Microbiology MCQ 2 - Question 1

Acid fast staining targets mycolic acid in the cell wall of Mycobacterium species. 

Microbiology MCQ 2 - Question 2

1 ml of microbial culture was diluted to 10ml and 1ml of diluted culture was further diluted by adding 99ml of buffer. 0.5 ml of final culture was plated on to an agar plate. Then, 200 colonies were observed. What was the number of bacteria per ml in the original culture?

Detailed Solution for Microbiology MCQ 2 - Question 2


Dilution factor
- 1 ml original diluted to 10ml means 10 times dilution
- 1 ml of diluted culture further diluted to 100 ml means 100 times dilution 
Total dilution factor = 1000 times = 10-3
Here. 0.5 ml of final culture contains 200 colonies

Microbiology MCQ 2 - Question 3

In a microbial culture, the maximum synthesis of antibiotics occurs in

Detailed Solution for Microbiology MCQ 2 - Question 3

Maximum synthesis of antibiotics in a microbial culture occurs in stationary phase because in stationary phase, nutrition and space become limitation. So to prevent excess growth in limited resources  and in this stress condition, microbes starts producing antibiotics (as a survival mechanism).

Microbiology MCQ 2 - Question 4

Which of the following is not true for photolithoautotrophic organism.

Detailed Solution for Microbiology MCQ 2 - Question 4

Photolithoautotrophs are the organisms which use
    - CO2 as carbon source (Autotrophs)
    - Inorganic ions / compounds as H+/e source (Litho)
    - Sunlight as energy source (Photo)

Microbiology MCQ 2 - Question 5

Find out the mismatch from the following.

Detailed Solution for Microbiology MCQ 2 - Question 5

Aerotolerant anaerobes grow equally well in the presence or absence of oxygen, eg. Streptococcus pyrogenes.

Microbiology MCQ 2 - Question 6

Correct sequence of events that occur during lytic cycle of bacteriophage infection.
P.    Synthesis of viral nucleic acid and protein
Q.    Release of new viruses by lysis of host cell
R.    Attachment of virus on the surface of bacteria
S.    Assembly and packaging of new virus
T.    Penetration of viral DNA into the host

Detailed Solution for Microbiology MCQ 2 - Question 6

Sequence of events that occur during lytic cycle of bacteriophage infection is,
(i)    Virus attaches on to the surface of the host bacterium.
(ii)    Penetration of viral DNA into the host
(iii)    Synthesis of viral nucleic acid and protein
(iv)    Assembly and packaging of new virus
(v)    Release of new virus by lysis of the host cell

Microbiology MCQ 2 - Question 7

Which of the following does not hold true for retroviruses.

Detailed Solution for Microbiology MCQ 2 - Question 7

Genome of retroviruses is single stranded RNA and not ds DNA.

Microbiology MCQ 2 - Question 8

Creutzfeldt Jacob disease in humans is caused by

Detailed Solution for Microbiology MCQ 2 - Question 8

Creutzfeldt Jacob disease in humans is caused by prions. Prions are infectious proteins with no nucleic acid.

Microbiology MCQ 2 - Question 9

Mode of action of penicillin is to inhibit 

Detailed Solution for Microbiology MCQ 2 - Question 9

Penicillin inhibits cell wall synthesis in bacteria by inhibiting the process of transpeptidation reaction, which is essential for cell wall synthesis.

Microbiology MCQ 2 - Question 10

Mode of action of streptomycin is by

Detailed Solution for Microbiology MCQ 2 - Question 10

Streptomycin inhibits translation in prokaryotes by preventing the formation of 70S ribosomes. It acts on the 30S subunit of prokaryotic ribosomes.

Microbiology MCQ 2 - Question 11

Gentamycin is an antibiotic, which is produced by

Detailed Solution for Microbiology MCQ 2 - Question 11

Gentamycin is an antibiotic produced by the bacteria belonging to Micromonospora sp. It inhibits translation by inhibiting 16s rRNA.

Microbiology MCQ 2 - Question 12

The presence of D-amino acids in the cross links of peptidoglycan layer is most likely because

Detailed Solution for Microbiology MCQ 2 - Question 12

D-amino acids are most likely present in cross links of peptidioglycan layer as proteases easily cleave L-amino acids.

*Multiple options can be correct
Microbiology MCQ 2 - Question 13

Which of the following is/are the characteristic(s) of exotoxin?

Detailed Solution for Microbiology MCQ 2 - Question 13

Exotoxins are produced by some species of gram positive and gram negative bacteria.
    - They are secreted out of the cell
    - They are heat labile, and get inactivated rapidly at 60 to 80ºC
    - They are polypeptides
    - They can be converted to toxoids and used as vaccines. 

*Multiple options can be correct
Microbiology MCQ 2 - Question 14

Select the disease(s) caused by bacteria.

Detailed Solution for Microbiology MCQ 2 - Question 14

Tetanus is caused by Clostridium tetani
    Anthrax is caused by Bacillus anthracis
    Whooping cough is caused by Bordetella pertusis    
    Meningococcal meningitis caused by Bordetella pertusis

*Multiple options can be correct
Microbiology MCQ 2 - Question 15

Which of the following disease (s) is/are caused by prions?

Detailed Solution for Microbiology MCQ 2 - Question 15

Kuru disease is caused by prions in humans. Scrapie disease is caused by prions in sheep.  

*Multiple options can be correct
Microbiology MCQ 2 - Question 16

HIV replicates its genome using unique mechanisms. Which of the following statements regarding the same are correct?

Detailed Solution for Microbiology MCQ 2 - Question 16

HIV is an enveloped virus and contains a RNA dependent DNA polymerase for infection. DNA copy of HIV genome is integrated into host cell DNA.

*Multiple options can be correct
Microbiology MCQ 2 - Question 17

Gram positive cells

Detailed Solution for Microbiology MCQ 2 - Question 17

All the characteristic present in options are correct regarding gram positive cells.

*Answer can only contain numeric values
Microbiology MCQ 2 - Question 18

A microorganism has a specific growth rate of 2.8 h–1, its doubling time will be _______ minutes. (Round off to two deimal places).
 


Detailed Solution for Microbiology MCQ 2 - Question 18

*Answer can only contain numeric values
Microbiology MCQ 2 - Question 19

A bacterium of cocci group has a diameter of 3µm . Calculate the ratio of its surface area to volume (in ) _________(Answer should be an integer)


Detailed Solution for Microbiology MCQ 2 - Question 19

Cocci bacterium has spherical shape. 
Surface area of sphere = 4ar2
Volume of sphere = 4/3ar2

*Answer can only contain numeric values
Microbiology MCQ 2 - Question 20

0.2 ml of a microbial culture was diluted by adding 9.8ml of buffer to 10 ml. 0.1ml of this was further diluted by adding 9.9 ml of buffer to 10 ml. Plating 0.5 ml from 2nd tube yields 80 colonies. Cell density in original culture was ____________   x 104 cells. (Round off to one decimal place)


Detailed Solution for Microbiology MCQ 2 - Question 20


*Answer can only contain numeric values
Microbiology MCQ 2 - Question 21

Volume of a single microbial cell is 10-3 um3. The number of cells present in its 5ml of culture will be_______________ 1015 cells. (Answer should be an integer)


Detailed Solution for Microbiology MCQ 2 - Question 21

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