Recognition sequence for some frequently used restriction endonucleases are given in the table. Find out which one is incorrectly matched.
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
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Which of the following is the proper order for vectors in terms of increasing cloning capacity?
If there is equal proportion of Auxin and Cytokinin in the medium for plant tissue growth then it will promote.
Which of the following partial amino acid sequence from a protein whose gene you wish to clone would be most useful in designing an oligonucleotide probe to screen a cDNA library.
P = Met-Lev-Arg-Leu, Q = Met-Trp-Cys-Trp
Match the technique with its appropriate application from the list of options given below:
Which of the following statements about the chaotrophic agent is incorrect?
What is drawback of Maxam-Gilbert method of DNA-sequencing?
What is the clinical application of monoclonal antibodies?
Consider the outcome of a density gradient centrifugation for the separation of biomecules.
Which of the following captions correctly represent the separated biomolecules in a density gradient tube
After using the Sanger method tor sequencing DNA (dideoxy nucleotide method), you observe the following autoradiogram
From the autoradiogram determine the base sequence of the template strand.
A restriction site “GAATTC” is recognized by an enzyme EcoRI, How many sites of this sequence are probable in a cloning vector, pBR322 of size 4361 bp.
Microscopy is the technique for making very small things visible to the naked eye. Find the corrected one
In any lab, a student performs a chymotrypsin digestion of the protein sequence
N-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.
What will be the result?
Consider the outcome of a reducing SDS-PAGE performed for a peptide performed from a purified fraction of a multisubunit peptide linked together by weak interactions as well as disulphide bridges. First lane represents the control group while the second lane represent proteins expressed under stress conditions. Considering that both samples had all the four peptides which were later confirmed by mass spectrometry.
Which of the following statements can be speculated to be correct from the given data
Choose the correct statements about the recombinant expression of proteins
Which of the following is not correct about restriction enzymes
λ EMBL3 is used as a vector to clone 2000 bp fragments generated from a partial Sau3Al digest of the human genome (3 x 109 bp). We wish to isolate a gene contained completely on a 20,000 bp fragment. To have a 99% chance of isolating this gene in the λ EMBL3 recombinant genomic library, the number of independent clones to be examined are _______
Hint: (2 x 104 / 3 x 109) = 6.7 x 10-6 and ln(1 - 6.7 x 10-6) = In 0.9999 and In (0.01) = -4.61
The given sequence of the polypeptide Ala-Arg-Tyr-Leu-Arg-Pro-Val-Trp-Lys-Pro is treated with Trypsin in the laboratory. The number of fragments generated after treatment with trypsin will be ______________
After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________
Reducing SDS-PAGE of IgM will produce ____________ bands in autoradiogram.