NEET Exam  >  NEET Tests  >  Biology Class 12  >  Test: Tools of Recombinant DNA Technology (NCERT) - NEET MCQ

Test: Tools of Recombinant DNA Technology (NCERT) - NEET MCQ


Test Description

30 Questions MCQ Test Biology Class 12 - Test: Tools of Recombinant DNA Technology (NCERT)

Test: Tools of Recombinant DNA Technology (NCERT) for NEET 2024 is part of Biology Class 12 preparation. The Test: Tools of Recombinant DNA Technology (NCERT) questions and answers have been prepared according to the NEET exam syllabus.The Test: Tools of Recombinant DNA Technology (NCERT) MCQs are made for NEET 2024 Exam. Find important definitions, questions, notes, meanings, examples, exercises, MCQs and online tests for Test: Tools of Recombinant DNA Technology (NCERT) below.
Solutions of Test: Tools of Recombinant DNA Technology (NCERT) questions in English are available as part of our Biology Class 12 for NEET & Test: Tools of Recombinant DNA Technology (NCERT) solutions in Hindi for Biology Class 12 course. Download more important topics, notes, lectures and mock test series for NEET Exam by signing up for free. Attempt Test: Tools of Recombinant DNA Technology (NCERT) | 30 questions in 30 minutes | Mock test for NEET preparation | Free important questions MCQ to study Biology Class 12 for NEET Exam | Download free PDF with solutions
Test: Tools of Recombinant DNA Technology (NCERT) - Question 1

The restriction enzyme responsible for the cleavage of following sequence  is 
5' - G - T - C - G - A - c - 3'
3' - C - A - G - C - T - G - 5'

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 1

HindII was the first discovered restriction endonuclease. It was isolated from Haemophilus influenzae. It produces blunt ends.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 2

Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 2

pBR322 was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez. It is widely used in gene cloning experiments. In pBR322 plasmid vector, p - denotes that it is a plasmid; BR - stands for Boliver and Rodriguez who constructed this plasmid; 322 - Is a number given to distinguish this plasmid from others developed in the same laboratory. E coli cloning vector pBR 322 has restriction sites {Hind III, Eco R I, Bam H l, SaI I, PvuI I, Pst I, Cla I), ori(origin of replication) and antibiotic resistance genes (ampR and tetR). rop codes for the proteins involved in the replication of the plasmid.

1 Crore+ students have signed up on EduRev. Have you? Download the App
Test: Tools of Recombinant DNA Technology (NCERT) - Question 3

Read the following statements and select the correct ones.
(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge
(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast
(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule
(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 3
  • Electrophoresis is a technique used for the separation of molecules based on their size and charge.
  • Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast.
  • It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule.
  • In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13.
Test: Tools of Recombinant DNA Technology (NCERT) - Question 4

Identify the palindromic sequence in the following.

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 4

The palindromes in DNA are base pair sequences that are the same when read forward (left to right) or backward (right to left) from a central axis of symmetry. Thus, ​ is a palindromic sequence.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 5

Which of the following statements is not correct regarding EcoRI restriction endonuclease enzyme?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 5

The restriction endonuclease enzyme EcoRI was isolated from bacterium Escherichia coli RY13. It recognises the base sequence GAATTC in DNA duplex and cuts its strands between G and A as shown below:

It results in complementary sticky ends.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 6

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 6

The cloning vector requires the presence of a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc. are considered useful selectable markers for E. coli. The normal E.coli cells do not carry resistance against any of these antibiotics.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 7

If you want to recover many copies of the target DNA, you will choose a vector

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 7

Vectors that have high number per cell will have high copy number of their genome within the bacterial cell. If we link an alien piece of DNA with vectorm we can multiply its number equal to the copy number of the vector. Any piece of DNA when linked to the 'ori' sequence, can be made to replicate within the host cells. This property of 'ori' is used to make a number of copies of the linked DNA. If we want to obtain many copies of the target DNA, then it should be cloned in such a vector whose 'ori' supports high copy number.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 8

The flow chart given below represents the process of recombinant DNA technology. Identify A, B, C and D

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 8

A and B are restriction endonucleases because same restrction enzyme cuts both foreign DNA and vector DNA at specific Point. C is DNA ligase which joins foreign DNA to vector DNA The newly formed recombinant DNA is transformed in bacteria and tha bacterial cells are allowed to divide.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 9

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 9

DNA  ligases are also called genetic gum. They join two individual fragments of double stranded DNA by forming phosphodiester bonds between them thus help in sealing of DNA fragments. Therefore acts as molecular glue. The enzyme used most often is T4DNA ligase.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 10

In recombinant DNA technology, the term vector refers to

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 10

The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA or cloning vector or gene carrier. When desired gene is introduced into a vector, recombinant DNA is formed. Vectors may be plasmids, bacteriophages, cosmids, phagemids, Yeast Artificial Chromosomes (Y ACs), Bacterial Artificial Chromosomes (BACs), transposons, viruses, etc.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 11

The term “competent” refers to

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 11

Transformation is a process by which a cell takes up naked DNA fragment from the environment, incorporates it into its own chromosomal DNA and finally expresses the trait controlled by the incoming DNA. Since DNA is a hydrophilic molecule, it can not pass through membranes, so the bacterial cells must be made competent to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium (Ca2+) which increases the efficiency with which DNA enters the bacterium through pores in its cell wall.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 12

The letter 'R' in EcoRI is derived from

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 12

In EcoRI, capital letter E comes from the genus Escherichia. The letters co are from the species coli. The letter R is from RY 13 (strain). The Roman number I indicates that it was the first enzyme isolated from the bacterium E.coli RY 13.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 13

How many fragments will be generated if you digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 13

If DNA is linear then the number of fragments generated is (N+1), where N= number of recognition sites or sequences. 
Hence the number of fragments generated is 5 if we digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 14

In the process of insertional inactivation

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 14

 A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzyme
Alternative selectable markers have been developed which differentiate recombinants from the non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the -galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 15

How many fragments will be generated on the digestion of a closed circular DNA molecule with a restriction enzyme having six recognition sites on the DNA?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 15

When a closed circular DNA molecule is digested with a restriction enzyme having six recognition sites, it will produce 6 DNA fragments.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 16

______ a crown gall bacterium, is called as 'natural genetic engineer' of plants.

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 16

A soil-inhabiting plant bacterium Agrobacterium tumefaciens is a pathogen of several dicot plants. It is able to transfer a piece of DNA known as 'T-DNA' into the plant cells. The T-DNA causes tumours, the tumours are called crown galls. Tumour formation is induced by Ti plasmid (Ti for tumour inducing). As gene transfer occurs without human effort, the bacterium is called natural genetic engineer of plants. Similarly retroviruses in animals including humans are able to change normal cells into cancerous cells.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 17

Which of the following is not used to transfer the recombinant DNA into the host?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 17

Bioreactors are considered as vessels in which raw materials are biologically converted into specific products by microbes, plant and animal cells or their enzymes. To produce large quantities of these products, bioreactors are used where large volumes (100-1000 litres) of culture can be processed. Bioreactor provides the optimal conditions for obtaining the desired product by providing optimum growth conditions such as temperature, pH, substrate, vitamins, oxygen and salts.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 18

Which of the following is required for microinjection method of gene transfer?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 18

Micro-injection method is the direct or vectorless method of gene transfer, in which foreign DNA is directly injected into the nucleus of animal cell or plant cell by using micro-needles or micro-pipettes. It is used to transfer DNA in oocytes, eggs and embryo.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 19

In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 19

Biolistic method or gene gun method is a direct or vectorless method of introducing DNA into cells that involves bombardment of cells with high-velocity microprojectiles coated with DNA. In biolistic method, tungsten or gold particles, coated with foreign DNA are bombarded into target at a very high velocity.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 20

Which of the following sequences is recognised by restriction enzyme Bam H I?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 20

Bam H I is obtained from Bacillus amyloliquefaciens H. It recognises a six base pair sequence and produces sticky ends.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 21

Which of the following is not a cloning vector?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 21

Sal l is a restriction enzyme isolated from Streptomyces albus.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 22

Which of the following statements is correct for molecular probes?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 22

Molecular probes are small DNA or RNA segments that are used to detect the presence of complementary sequences in nucleic acid samples. These are usually formed of 200-500 nucleotide sequences but may have up to 500 nucleotides. These segments or probes are labelled either with radioactive or with nonradioactive compound. Single stranded DNA probes, denatured double stranded DNA probes and RNA probes are used for identification and isolation of DNA and RNA.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 23

What is the effect if pBR322, a cloning vector does not carry 'ori site'?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 23

For the multiplication of any alien piece of DNA in an organism, it needs to be a part of a chromosome which has a specific sequence known as 'origin of replication' (ori). If ori is not present in a cloning vector, replication will not be initiated.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 24

In pBR322, tetracycline resistance gene (tetR) has recognition site for which of the following restriction endonuclease?

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 24

In plasmid vector pBR322, two unique restriction sites Pst I arid Pvu I are located within the ampR gene and Bam H l, SaI I, etc., are located within the tetR gene. The presence of restriction sites within the marker genes tetR and ampR permits an easy selection for cells transformed with the I recombinant pBR322. When restriction enzyme Bam H l or SaI I is used, the DNA insert is placed within the gene tetR making it nonfunctional.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 25

Read the given statements and select the correct option. 
Statement 1:
The tumour inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
Statements 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 25

A tumour inducing Ti plasmid of Agrobacterium tumefaciens has been modified into a cloning vector which is not pathogenic to the plants, However, it is still able to use its mechanism to deliver genes of our interest into various plants.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 26

The sticky ends of a fragmented DNA molecule are made of

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 26

The single-stranded free ends that project from each fragment of DNA duplex are unpaired bases and are known as "sticky ends". Sticky ends can join with similar complementary ends of DNA fragment from some other sources.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 27

Match the terms given in column I with their definitions in column II and select the correct answer from codes given below.

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 27

Transformation is the process of genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through cell membrane. 
Recognition site or restriction sites are locations on a DNA molecule containing specific sequences of nucleotides which are recognised by restriction enzymes. 
Gel electrophoresis is a process used to separate DNA fragments according to their size and charge. 
Recombinant DNA are the DNA molecules from the different species that are inserted into a host to produce new genetic combinations.

So, the correct answer is A-4, B-1, C-2, D-3.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 28

If a plasmid vector is digested with EcoRI at a single site, then

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 28

Plasmid is a circular DNA, if it is digested at a single site, one fragment will be produced with two sticky ends.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 29

Read the given statements and select the correct option.
Statement 1:
The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 29

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' produced, which can be joined together (end-to-end) using DNA ligase. Restriction enzymes are of two kinds - exonucleases and endonucleases. Exonucleases remove nucleotides from the j ends of the DNA whereas endonucleases make cuts at specific positions within the DNA. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. Therefore, in order to link the alien DNA (or foreign DNA), the vector needs to have very few, preferably single, recognition/cloning sites for the commonly used restriction enzymes.

Test: Tools of Recombinant DNA Technology (NCERT) - Question 30

A correct pair of characteristics of molecular probe is
A. Very long molecule
B. Double stranded
C. Single stranded DNA or RNA
D. Complementary to part of desired gene

Detailed Solution for Test: Tools of Recombinant DNA Technology (NCERT) - Question 30

Molecular probes are small single stranded DNA or RNA segments that are used to detect the presence of complementary sequences in nucleic acid samples. These are usually formed of 200-500 nucleotide sequences. These segments or probes are labelled either with radioactive or with nonradioactive compound. Single stranded DNA / RNA probes are used for identification and isolation of DNA and RNA.

122 videos|311 docs|196 tests
Information about Test: Tools of Recombinant DNA Technology (NCERT) Page
In this test you can find the Exam questions for Test: Tools of Recombinant DNA Technology (NCERT) solved & explained in the simplest way possible. Besides giving Questions and answers for Test: Tools of Recombinant DNA Technology (NCERT), EduRev gives you an ample number of Online tests for practice

Up next

122 videos|311 docs|196 tests
Download as PDF

Up next