Test: Tools of Recombinant DNA Technology: Competent Host - NEET MCQ

The molecule which dissolves in water is called hydrophilic molecules. They are polar in nature which helps them in dissolution in water. Being hydrophilic in nature DNA cannot pass through the cell membrane.

DNA is an example of a hydrophilic molecule. Hydrophilic molecules are water-loving and tend to dissolve in water easily. They carry a positive or negative charge.

The divalent cations of calcium are used to make cells competent. Calcium is used in the form of a salt of calcium chloride. It increases the efficiency of the bacterial membrane to allow the passage of foreign DNA.

Some bacterial cells have the ability to incorporate foreign DNA within them. These cells are called competent cells. This leads to the alteration of the genetic material of the cell.

Heat-shock treatment is a method in which the vial is incubated in ice, then treated at temperature up to 50°C and again transferred on ice. It is usually used in transferring recombinant DNA within the host (bacterial cell).

The method by which recombinant DNA is directly injected into the nucleus of an animal cell is called micro-injection. A glass micropipette is used for injecting the material into cells.

Biolistics is also known as a gene gun. It is a method in which cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA. It is mostly used for plant cells.

42°C-45°C is the ideal temperature for heat-shock treatment. Mostly calcium chloride is used in this. It is suitable for the transformation of bacterial cells such as E.coli.

Calcium is a divalent cation. It has a valency of 2 and is written as Cu²⁺. Some other examples of divalent atoms are Zn²⁺, Mg²⁺, Co²⁺, etc.

The microparticles of gold-coated with DNA are used in gene gun technique. Aurum is a Latin word for gold. Other metal used for this technique is tungsten.