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IIT JAM BIOTECHNOLOGY 2021  
SECTION A 
Q.1 Ecosystem ecology is the study of 
(1) Factors that affect the interactions of 
individuals in a population 
(2) Interactions among biotic and abiotic 
components 
(3) An organism’s behavior towards 
environmental challenges 
(4) Factors that affect the interactions among 
communities in an ecosystem 
 
Q.2 Which one of the following features 
distinguishes between gymnosperms and 
angiosperms? 
(1) Vascular tissues 
(2) Seed cover 
(3) Gamete production 
(4) Seed formation 
 
Q.3 If the blood groups of mother and father 
we AB and O respectively, what are the blood 
groups possible for their child 
(1) AB or A 
(2) AB, A, B or O 
(3) AB 
(4) A or B 
 
Q.4 Ethyl butyrate is responsible for the odor 
of pineapple. Which one of the following is 
the structure of ethyl butyrate?(ANS. 2) 
 
 
 
 
 
Q.5 Bacterial strains that do not grow in the 
absence of a specific nutrient are called 
(1) Heterotrophs 
(2) Autotrophs 
(3) Chemotrophs 
(4)Auxotrophs 
 
Q.6 Let ??= (
?? ?? ?? ?? ) and ??= (
?? -?? ?? ?? ). If 
???? +???? =?? , then the determinant of ?? is 
(1) 6 
(2) 18 
(3) -18 
(4) -6 
 
Q.7 The moment of force in terms of 
fundamental dimensions is 
(1) MLT
-1
 
(2) ML
2
T
-2
 
(3) ML
-1
T
-1
 
(4) MLT
-2
 
 
Q.8 The type of immunological protection 
provided by plasma therapy is 
(1) Natural passive 
(2) Artificial active 
(3) Natural active 
(4) Artificial passive 
 
Q.9 Which one of the following components 
of bacterial cell acts as endotoxin? 
(1) Peptidoglycan of Gram-negative bacteria 
(2) Lipopolysaccharide 
(3) Porins 
(4) Peptidoglycan of Gram-positive bacteria 
 
Q.10 An acid contains C, H and O atoms. On 
combustion analysis. 0.454 g of the acid gives 
0.418 g ofH 20 and 1.023 g of CO 2 What is the 
empirical formula of the acid? 
(1) CH 2O 
(2) C 4H 5O2 
(3) C 5H 8O 
(4) C 3H 6O 
 
 
Page 2


IIT JAM BIOTECHNOLOGY 2021  
SECTION A 
Q.1 Ecosystem ecology is the study of 
(1) Factors that affect the interactions of 
individuals in a population 
(2) Interactions among biotic and abiotic 
components 
(3) An organism’s behavior towards 
environmental challenges 
(4) Factors that affect the interactions among 
communities in an ecosystem 
 
Q.2 Which one of the following features 
distinguishes between gymnosperms and 
angiosperms? 
(1) Vascular tissues 
(2) Seed cover 
(3) Gamete production 
(4) Seed formation 
 
Q.3 If the blood groups of mother and father 
we AB and O respectively, what are the blood 
groups possible for their child 
(1) AB or A 
(2) AB, A, B or O 
(3) AB 
(4) A or B 
 
Q.4 Ethyl butyrate is responsible for the odor 
of pineapple. Which one of the following is 
the structure of ethyl butyrate?(ANS. 2) 
 
 
 
 
 
Q.5 Bacterial strains that do not grow in the 
absence of a specific nutrient are called 
(1) Heterotrophs 
(2) Autotrophs 
(3) Chemotrophs 
(4)Auxotrophs 
 
Q.6 Let ??= (
?? ?? ?? ?? ) and ??= (
?? -?? ?? ?? ). If 
???? +???? =?? , then the determinant of ?? is 
(1) 6 
(2) 18 
(3) -18 
(4) -6 
 
Q.7 The moment of force in terms of 
fundamental dimensions is 
(1) MLT
-1
 
(2) ML
2
T
-2
 
(3) ML
-1
T
-1
 
(4) MLT
-2
 
 
Q.8 The type of immunological protection 
provided by plasma therapy is 
(1) Natural passive 
(2) Artificial active 
(3) Natural active 
(4) Artificial passive 
 
Q.9 Which one of the following components 
of bacterial cell acts as endotoxin? 
(1) Peptidoglycan of Gram-negative bacteria 
(2) Lipopolysaccharide 
(3) Porins 
(4) Peptidoglycan of Gram-positive bacteria 
 
Q.10 An acid contains C, H and O atoms. On 
combustion analysis. 0.454 g of the acid gives 
0.418 g ofH 20 and 1.023 g of CO 2 What is the 
empirical formula of the acid? 
(1) CH 2O 
(2) C 4H 5O2 
(3) C 5H 8O 
(4) C 3H 6O 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.11 What is the significance of 
theisomerization of glucose 6 phosphate to 
fructose 6-phosphate for the progression of 
glycolysis  
(1) As functional groups, ketones are more 
reactive than aldehydes 
(2) Phosphorylation of glucose 6-phosphate to 
glucose 1,6-bisphosphate is irreversible 
(3) Cleavage of glucose 1,6-bisphosphate will 
not yield dihydroxy acetone phosphate and 
glyceraldehyde 3-phosphate 
(4) The carbonyl group at carbon-2 (C-2) in 
fructose facilitates the cleavage of the bond 
between C-3 and 4 
 
Q.12Which one of the following processes 
emerged earliest during the course of 
evolution? 
(1) Antigen presentation 
(2) Antibody production 
(3) Thymic education 
(4) Phagocytosis 
 
Q.13A stationary enemy ship is docked in the 
sea at a distance of 1.0 km from the coastline. 
A gunlocated at the sea level on the coastline 
can fire projectiles at a velocity of 120 m/s. 
What is theangle in degrees) above 
thehorizontal at which the gun must fire to 
hit the ship? [g = 9.8 m/s
2
] 
(1)47.1 
(2)42.9 
(3) 23.6 
(4)21.4 
 
Q.14 In mammals, females have two X 
chromosomes and males have one X 
chromosome. Equal expression of X 
chromosome genes in both sexes is ensured 
by  
(1) Heterochromatin formation 
(2) RNA silencing 
(3) Dosage compensation 
(4) Histone code 
 
 
 
Q.15 What is the role of bile salts in the 
mammalian digestive system? 
(1) Bile salts emulsify fat, and thus aid in fat 
digestion 
(2) Bile salts convert pepsinogen to pepsin, and 
thus facilitate protein digestion 
(3) Bile salts are excretory products produced 
by the liver, and do not participate in digestion 
(4) Bile salts facilitate digestion of all types of 
macromolecules in the small intestine 
 
Q.16 The value of the integral ? (?? -
?? ?? ?? (?? ))???? , where ?? (?? )=
{
 
 
 
 
?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? , is 
(1)-2 
(2) 2 
(3) 1 
(4)-1 
 
Q.17 Match the cell junctions listed in Group A 
with their correct functions listed in Group B: 
 
Group A Group B 
(I). Adherens 
junction 
(P). Joins actin bundles 
in neighboring cells 
(II). Desmosome (Q). Joins intermediate 
filaments in 
neighboring cells 
(III). Tight junction (R). Seals neighboring 
cells 
(IV). Gap junction (S). Allows diffusion of 
molecules between 
adjacent cells 
 
(1) I-Q; II-R; III-S; IV-P 
(2) I-P; II-Q; III-R; IV-S 
(3) I-Q; II-R; III-P; IV-S 
(4) I-S; II-P; III-Q; IV-R 
 
 
 
 
 
Page 3


IIT JAM BIOTECHNOLOGY 2021  
SECTION A 
Q.1 Ecosystem ecology is the study of 
(1) Factors that affect the interactions of 
individuals in a population 
(2) Interactions among biotic and abiotic 
components 
(3) An organism’s behavior towards 
environmental challenges 
(4) Factors that affect the interactions among 
communities in an ecosystem 
 
Q.2 Which one of the following features 
distinguishes between gymnosperms and 
angiosperms? 
(1) Vascular tissues 
(2) Seed cover 
(3) Gamete production 
(4) Seed formation 
 
Q.3 If the blood groups of mother and father 
we AB and O respectively, what are the blood 
groups possible for their child 
(1) AB or A 
(2) AB, A, B or O 
(3) AB 
(4) A or B 
 
Q.4 Ethyl butyrate is responsible for the odor 
of pineapple. Which one of the following is 
the structure of ethyl butyrate?(ANS. 2) 
 
 
 
 
 
Q.5 Bacterial strains that do not grow in the 
absence of a specific nutrient are called 
(1) Heterotrophs 
(2) Autotrophs 
(3) Chemotrophs 
(4)Auxotrophs 
 
Q.6 Let ??= (
?? ?? ?? ?? ) and ??= (
?? -?? ?? ?? ). If 
???? +???? =?? , then the determinant of ?? is 
(1) 6 
(2) 18 
(3) -18 
(4) -6 
 
Q.7 The moment of force in terms of 
fundamental dimensions is 
(1) MLT
-1
 
(2) ML
2
T
-2
 
(3) ML
-1
T
-1
 
(4) MLT
-2
 
 
Q.8 The type of immunological protection 
provided by plasma therapy is 
(1) Natural passive 
(2) Artificial active 
(3) Natural active 
(4) Artificial passive 
 
Q.9 Which one of the following components 
of bacterial cell acts as endotoxin? 
(1) Peptidoglycan of Gram-negative bacteria 
(2) Lipopolysaccharide 
(3) Porins 
(4) Peptidoglycan of Gram-positive bacteria 
 
Q.10 An acid contains C, H and O atoms. On 
combustion analysis. 0.454 g of the acid gives 
0.418 g ofH 20 and 1.023 g of CO 2 What is the 
empirical formula of the acid? 
(1) CH 2O 
(2) C 4H 5O2 
(3) C 5H 8O 
(4) C 3H 6O 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.11 What is the significance of 
theisomerization of glucose 6 phosphate to 
fructose 6-phosphate for the progression of 
glycolysis  
(1) As functional groups, ketones are more 
reactive than aldehydes 
(2) Phosphorylation of glucose 6-phosphate to 
glucose 1,6-bisphosphate is irreversible 
(3) Cleavage of glucose 1,6-bisphosphate will 
not yield dihydroxy acetone phosphate and 
glyceraldehyde 3-phosphate 
(4) The carbonyl group at carbon-2 (C-2) in 
fructose facilitates the cleavage of the bond 
between C-3 and 4 
 
Q.12Which one of the following processes 
emerged earliest during the course of 
evolution? 
(1) Antigen presentation 
(2) Antibody production 
(3) Thymic education 
(4) Phagocytosis 
 
Q.13A stationary enemy ship is docked in the 
sea at a distance of 1.0 km from the coastline. 
A gunlocated at the sea level on the coastline 
can fire projectiles at a velocity of 120 m/s. 
What is theangle in degrees) above 
thehorizontal at which the gun must fire to 
hit the ship? [g = 9.8 m/s
2
] 
(1)47.1 
(2)42.9 
(3) 23.6 
(4)21.4 
 
Q.14 In mammals, females have two X 
chromosomes and males have one X 
chromosome. Equal expression of X 
chromosome genes in both sexes is ensured 
by  
(1) Heterochromatin formation 
(2) RNA silencing 
(3) Dosage compensation 
(4) Histone code 
 
 
 
Q.15 What is the role of bile salts in the 
mammalian digestive system? 
(1) Bile salts emulsify fat, and thus aid in fat 
digestion 
(2) Bile salts convert pepsinogen to pepsin, and 
thus facilitate protein digestion 
(3) Bile salts are excretory products produced 
by the liver, and do not participate in digestion 
(4) Bile salts facilitate digestion of all types of 
macromolecules in the small intestine 
 
Q.16 The value of the integral ? (?? -
?? ?? ?? (?? ))???? , where ?? (?? )=
{
 
 
 
 
?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? , is 
(1)-2 
(2) 2 
(3) 1 
(4)-1 
 
Q.17 Match the cell junctions listed in Group A 
with their correct functions listed in Group B: 
 
Group A Group B 
(I). Adherens 
junction 
(P). Joins actin bundles 
in neighboring cells 
(II). Desmosome (Q). Joins intermediate 
filaments in 
neighboring cells 
(III). Tight junction (R). Seals neighboring 
cells 
(IV). Gap junction (S). Allows diffusion of 
molecules between 
adjacent cells 
 
(1) I-Q; II-R; III-S; IV-P 
(2) I-P; II-Q; III-R; IV-S 
(3) I-Q; II-R; III-P; IV-S 
(4) I-S; II-P; III-Q; IV-R 
 
 
 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.18  The following methylation is carried out 
in various solvents such as 
benzene,tetrahydrofuran (THF), 
dimethoxyethane (DME), dimethyl sulfoxide 
(DMSO) and N,N-dimethylformamide (DMF). 
Which one of the following is TRUE for the 
effect of solvent on the reaction rate? 
 
(1) THE > Benzene > DME > DMSO > DMF 
(2) DME > DMSO > DMF > THF > Benzene 
(3) DMSO > DMF > DME > THF > Benzene 
(4) Benzene > THF > DME > DMF > DMSO 
 
Q.19 Which one of the following is the major 
product of the hydrogenation reaction given 
below? (ANS.1) 
 
 
Q.20 Which one of the following features 
properties does glucose acquire through 
intramolecularhemiacetal formation? 
(1) Ability to form anhydride linkage with non-
carbohydrate moieties such as the 
inorganicphosphate 
(2) Ability to form epimers 
(3) An additional chiral carbon 
(4) Ability to function as a reducing agent 
 
Q.21 Presence of which one of the following 
in the urine indicates pregnancy in human? 
(1) Progesterone 
(2) Human chorionic gonadotropin 
(3) Estrogen 
(4)Follicle-stimulating hormone and 
luteinizinghormone 
 
Q.22 In plants, the ovules are attached to the 
ovary by 
(1) Embryo sac 
(2)Synergids 
(3) Placenta 
(4) Tube cells 
 
Q.23 Which one of the following isomers is 
thermodynamically most stable? (ANS.1) 
 
 
Q.24 Which one of the following microscopic 
techniques provides a 3-dimensional 
perspective of live,unstained and transparent 
specimens obtained from the wild? 
(1) Phase contrast microscopy 
(2) Confocal microscopy  
(3) Differential interference contrast 
(Nomarski)microscopy 
(4) Fluorescence microscopy 
 
Q.25 IUPAC name of the following molecule is 
 
(1) 3-Bromo-3,5-dimethyl hexane 
(2) 3-Bromo-2-isobutyl butane 
(3)4-Bromo-2,4-dimethyl hexane 
(4) 4-Bromo-2-methyl-4-ethyl pentane 
 
Q.26 The lack of linear correlation between 
the genome sizes and genetic complexities 
among variousspecies is known as 
(1) Genetic diversity 
(2) Central dogma 
(3) G-value paradox 
(4) C-value paradox 
 
 
 
Page 4


IIT JAM BIOTECHNOLOGY 2021  
SECTION A 
Q.1 Ecosystem ecology is the study of 
(1) Factors that affect the interactions of 
individuals in a population 
(2) Interactions among biotic and abiotic 
components 
(3) An organism’s behavior towards 
environmental challenges 
(4) Factors that affect the interactions among 
communities in an ecosystem 
 
Q.2 Which one of the following features 
distinguishes between gymnosperms and 
angiosperms? 
(1) Vascular tissues 
(2) Seed cover 
(3) Gamete production 
(4) Seed formation 
 
Q.3 If the blood groups of mother and father 
we AB and O respectively, what are the blood 
groups possible for their child 
(1) AB or A 
(2) AB, A, B or O 
(3) AB 
(4) A or B 
 
Q.4 Ethyl butyrate is responsible for the odor 
of pineapple. Which one of the following is 
the structure of ethyl butyrate?(ANS. 2) 
 
 
 
 
 
Q.5 Bacterial strains that do not grow in the 
absence of a specific nutrient are called 
(1) Heterotrophs 
(2) Autotrophs 
(3) Chemotrophs 
(4)Auxotrophs 
 
Q.6 Let ??= (
?? ?? ?? ?? ) and ??= (
?? -?? ?? ?? ). If 
???? +???? =?? , then the determinant of ?? is 
(1) 6 
(2) 18 
(3) -18 
(4) -6 
 
Q.7 The moment of force in terms of 
fundamental dimensions is 
(1) MLT
-1
 
(2) ML
2
T
-2
 
(3) ML
-1
T
-1
 
(4) MLT
-2
 
 
Q.8 The type of immunological protection 
provided by plasma therapy is 
(1) Natural passive 
(2) Artificial active 
(3) Natural active 
(4) Artificial passive 
 
Q.9 Which one of the following components 
of bacterial cell acts as endotoxin? 
(1) Peptidoglycan of Gram-negative bacteria 
(2) Lipopolysaccharide 
(3) Porins 
(4) Peptidoglycan of Gram-positive bacteria 
 
Q.10 An acid contains C, H and O atoms. On 
combustion analysis. 0.454 g of the acid gives 
0.418 g ofH 20 and 1.023 g of CO 2 What is the 
empirical formula of the acid? 
(1) CH 2O 
(2) C 4H 5O2 
(3) C 5H 8O 
(4) C 3H 6O 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.11 What is the significance of 
theisomerization of glucose 6 phosphate to 
fructose 6-phosphate for the progression of 
glycolysis  
(1) As functional groups, ketones are more 
reactive than aldehydes 
(2) Phosphorylation of glucose 6-phosphate to 
glucose 1,6-bisphosphate is irreversible 
(3) Cleavage of glucose 1,6-bisphosphate will 
not yield dihydroxy acetone phosphate and 
glyceraldehyde 3-phosphate 
(4) The carbonyl group at carbon-2 (C-2) in 
fructose facilitates the cleavage of the bond 
between C-3 and 4 
 
Q.12Which one of the following processes 
emerged earliest during the course of 
evolution? 
(1) Antigen presentation 
(2) Antibody production 
(3) Thymic education 
(4) Phagocytosis 
 
Q.13A stationary enemy ship is docked in the 
sea at a distance of 1.0 km from the coastline. 
A gunlocated at the sea level on the coastline 
can fire projectiles at a velocity of 120 m/s. 
What is theangle in degrees) above 
thehorizontal at which the gun must fire to 
hit the ship? [g = 9.8 m/s
2
] 
(1)47.1 
(2)42.9 
(3) 23.6 
(4)21.4 
 
Q.14 In mammals, females have two X 
chromosomes and males have one X 
chromosome. Equal expression of X 
chromosome genes in both sexes is ensured 
by  
(1) Heterochromatin formation 
(2) RNA silencing 
(3) Dosage compensation 
(4) Histone code 
 
 
 
Q.15 What is the role of bile salts in the 
mammalian digestive system? 
(1) Bile salts emulsify fat, and thus aid in fat 
digestion 
(2) Bile salts convert pepsinogen to pepsin, and 
thus facilitate protein digestion 
(3) Bile salts are excretory products produced 
by the liver, and do not participate in digestion 
(4) Bile salts facilitate digestion of all types of 
macromolecules in the small intestine 
 
Q.16 The value of the integral ? (?? -
?? ?? ?? (?? ))???? , where ?? (?? )=
{
 
 
 
 
?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? , is 
(1)-2 
(2) 2 
(3) 1 
(4)-1 
 
Q.17 Match the cell junctions listed in Group A 
with their correct functions listed in Group B: 
 
Group A Group B 
(I). Adherens 
junction 
(P). Joins actin bundles 
in neighboring cells 
(II). Desmosome (Q). Joins intermediate 
filaments in 
neighboring cells 
(III). Tight junction (R). Seals neighboring 
cells 
(IV). Gap junction (S). Allows diffusion of 
molecules between 
adjacent cells 
 
(1) I-Q; II-R; III-S; IV-P 
(2) I-P; II-Q; III-R; IV-S 
(3) I-Q; II-R; III-P; IV-S 
(4) I-S; II-P; III-Q; IV-R 
 
 
 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.18  The following methylation is carried out 
in various solvents such as 
benzene,tetrahydrofuran (THF), 
dimethoxyethane (DME), dimethyl sulfoxide 
(DMSO) and N,N-dimethylformamide (DMF). 
Which one of the following is TRUE for the 
effect of solvent on the reaction rate? 
 
(1) THE > Benzene > DME > DMSO > DMF 
(2) DME > DMSO > DMF > THF > Benzene 
(3) DMSO > DMF > DME > THF > Benzene 
(4) Benzene > THF > DME > DMF > DMSO 
 
Q.19 Which one of the following is the major 
product of the hydrogenation reaction given 
below? (ANS.1) 
 
 
Q.20 Which one of the following features 
properties does glucose acquire through 
intramolecularhemiacetal formation? 
(1) Ability to form anhydride linkage with non-
carbohydrate moieties such as the 
inorganicphosphate 
(2) Ability to form epimers 
(3) An additional chiral carbon 
(4) Ability to function as a reducing agent 
 
Q.21 Presence of which one of the following 
in the urine indicates pregnancy in human? 
(1) Progesterone 
(2) Human chorionic gonadotropin 
(3) Estrogen 
(4)Follicle-stimulating hormone and 
luteinizinghormone 
 
Q.22 In plants, the ovules are attached to the 
ovary by 
(1) Embryo sac 
(2)Synergids 
(3) Placenta 
(4) Tube cells 
 
Q.23 Which one of the following isomers is 
thermodynamically most stable? (ANS.1) 
 
 
Q.24 Which one of the following microscopic 
techniques provides a 3-dimensional 
perspective of live,unstained and transparent 
specimens obtained from the wild? 
(1) Phase contrast microscopy 
(2) Confocal microscopy  
(3) Differential interference contrast 
(Nomarski)microscopy 
(4) Fluorescence microscopy 
 
Q.25 IUPAC name of the following molecule is 
 
(1) 3-Bromo-3,5-dimethyl hexane 
(2) 3-Bromo-2-isobutyl butane 
(3)4-Bromo-2,4-dimethyl hexane 
(4) 4-Bromo-2-methyl-4-ethyl pentane 
 
Q.26 The lack of linear correlation between 
the genome sizes and genetic complexities 
among variousspecies is known as 
(1) Genetic diversity 
(2) Central dogma 
(3) G-value paradox 
(4) C-value paradox 
 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.27 In a genetic cross between plants 
bearing violet flowers and green seeds 
(VVGG), and white flower and yellow seeds 
(rygg), thefollowing phenotypic distribution 
was obtained in the F2 progeny(assume both 
parents to be pure breeding for both the 
traits, and self-cross at F1 generation):  
i) 2340 plants with violet flowers and green 
seeds 
ii) 47 plants with violet flowers and yellow 
seeds 
iii) 43 plants with white flowers and green 
seeds 
iv) 770 plants with white flowers and yellow 
seeds 
Which one of the following interpretations 
explains the above phenotypic distribution? 
(1) Flower color in this plant species is a 
polygenic trait 
(2) Genes for flower and seed colors are 
present on the same chromosome 
(3) Same genes control both flower and seed 
colors 
(4) Genes for flower and seed colors are 
genetically interacting 
 
Q.28 Which one of the following represents 
the motion of an object with a positive 
acceleration?(ANS.4) 
 
 
 
 
 
 
 
Q.29 The difference between mitosis and 
meiosis I is 
(1) The DNA is double helical in meiosis I but 
not in mitosis 
(2) Sister chromatids separate in mitosis, 
whereas homologous chromosomes separate 
in meiosis I 
(3) Unlike in mitotic metaphase, chromosomes 
do not align at the equatorial plate in meiosis I 
(4)The nuclear membrane is absent during 
mitotic metaphase, but not in meiotic 
metaphase 
 
Q.30 If ?? +
?? ?? =?? , then the value of ?? ?? +
?? ?? ?? is 
(1)-2 
(2) 2 
(3) 1 
(4)-1 
 
B Section 
Q.1Which of the following is/are TRUE about 
the electron carrier, ubiquinone (coenzyme 
Q)? 
(1)Its hydrophilic nature and high affinity for 
protons enable ubiquinone to transport 
protonsreadily within the intermembrane 
space of mitochondria 
(2)Its ability to accept two electrons, one at a 
time, enables ubiquinone to function at the 
junctionbetween a 2-electron donor and a 1-
electron acceptor 
(3)Being small and hydrophobic, ubiquinone 
readily shuttles between protein-
basedelectrontransfer complexes within the 
membrane 
(4)Its ability to interact with Heme of 
cytochromes enables electron transport in 
themitochondrial membrane 
 
 
 
 
 
 
 
 
Page 5


IIT JAM BIOTECHNOLOGY 2021  
SECTION A 
Q.1 Ecosystem ecology is the study of 
(1) Factors that affect the interactions of 
individuals in a population 
(2) Interactions among biotic and abiotic 
components 
(3) An organism’s behavior towards 
environmental challenges 
(4) Factors that affect the interactions among 
communities in an ecosystem 
 
Q.2 Which one of the following features 
distinguishes between gymnosperms and 
angiosperms? 
(1) Vascular tissues 
(2) Seed cover 
(3) Gamete production 
(4) Seed formation 
 
Q.3 If the blood groups of mother and father 
we AB and O respectively, what are the blood 
groups possible for their child 
(1) AB or A 
(2) AB, A, B or O 
(3) AB 
(4) A or B 
 
Q.4 Ethyl butyrate is responsible for the odor 
of pineapple. Which one of the following is 
the structure of ethyl butyrate?(ANS. 2) 
 
 
 
 
 
Q.5 Bacterial strains that do not grow in the 
absence of a specific nutrient are called 
(1) Heterotrophs 
(2) Autotrophs 
(3) Chemotrophs 
(4)Auxotrophs 
 
Q.6 Let ??= (
?? ?? ?? ?? ) and ??= (
?? -?? ?? ?? ). If 
???? +???? =?? , then the determinant of ?? is 
(1) 6 
(2) 18 
(3) -18 
(4) -6 
 
Q.7 The moment of force in terms of 
fundamental dimensions is 
(1) MLT
-1
 
(2) ML
2
T
-2
 
(3) ML
-1
T
-1
 
(4) MLT
-2
 
 
Q.8 The type of immunological protection 
provided by plasma therapy is 
(1) Natural passive 
(2) Artificial active 
(3) Natural active 
(4) Artificial passive 
 
Q.9 Which one of the following components 
of bacterial cell acts as endotoxin? 
(1) Peptidoglycan of Gram-negative bacteria 
(2) Lipopolysaccharide 
(3) Porins 
(4) Peptidoglycan of Gram-positive bacteria 
 
Q.10 An acid contains C, H and O atoms. On 
combustion analysis. 0.454 g of the acid gives 
0.418 g ofH 20 and 1.023 g of CO 2 What is the 
empirical formula of the acid? 
(1) CH 2O 
(2) C 4H 5O2 
(3) C 5H 8O 
(4) C 3H 6O 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.11 What is the significance of 
theisomerization of glucose 6 phosphate to 
fructose 6-phosphate for the progression of 
glycolysis  
(1) As functional groups, ketones are more 
reactive than aldehydes 
(2) Phosphorylation of glucose 6-phosphate to 
glucose 1,6-bisphosphate is irreversible 
(3) Cleavage of glucose 1,6-bisphosphate will 
not yield dihydroxy acetone phosphate and 
glyceraldehyde 3-phosphate 
(4) The carbonyl group at carbon-2 (C-2) in 
fructose facilitates the cleavage of the bond 
between C-3 and 4 
 
Q.12Which one of the following processes 
emerged earliest during the course of 
evolution? 
(1) Antigen presentation 
(2) Antibody production 
(3) Thymic education 
(4) Phagocytosis 
 
Q.13A stationary enemy ship is docked in the 
sea at a distance of 1.0 km from the coastline. 
A gunlocated at the sea level on the coastline 
can fire projectiles at a velocity of 120 m/s. 
What is theangle in degrees) above 
thehorizontal at which the gun must fire to 
hit the ship? [g = 9.8 m/s
2
] 
(1)47.1 
(2)42.9 
(3) 23.6 
(4)21.4 
 
Q.14 In mammals, females have two X 
chromosomes and males have one X 
chromosome. Equal expression of X 
chromosome genes in both sexes is ensured 
by  
(1) Heterochromatin formation 
(2) RNA silencing 
(3) Dosage compensation 
(4) Histone code 
 
 
 
Q.15 What is the role of bile salts in the 
mammalian digestive system? 
(1) Bile salts emulsify fat, and thus aid in fat 
digestion 
(2) Bile salts convert pepsinogen to pepsin, and 
thus facilitate protein digestion 
(3) Bile salts are excretory products produced 
by the liver, and do not participate in digestion 
(4) Bile salts facilitate digestion of all types of 
macromolecules in the small intestine 
 
Q.16 The value of the integral ? (?? -
?? ?? ?? (?? ))???? , where ?? (?? )=
{
 
 
 
 
?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? ?? , ?? = ?? < ?? , is 
(1)-2 
(2) 2 
(3) 1 
(4)-1 
 
Q.17 Match the cell junctions listed in Group A 
with their correct functions listed in Group B: 
 
Group A Group B 
(I). Adherens 
junction 
(P). Joins actin bundles 
in neighboring cells 
(II). Desmosome (Q). Joins intermediate 
filaments in 
neighboring cells 
(III). Tight junction (R). Seals neighboring 
cells 
(IV). Gap junction (S). Allows diffusion of 
molecules between 
adjacent cells 
 
(1) I-Q; II-R; III-S; IV-P 
(2) I-P; II-Q; III-R; IV-S 
(3) I-Q; II-R; III-P; IV-S 
(4) I-S; II-P; III-Q; IV-R 
 
 
 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.18  The following methylation is carried out 
in various solvents such as 
benzene,tetrahydrofuran (THF), 
dimethoxyethane (DME), dimethyl sulfoxide 
(DMSO) and N,N-dimethylformamide (DMF). 
Which one of the following is TRUE for the 
effect of solvent on the reaction rate? 
 
(1) THE > Benzene > DME > DMSO > DMF 
(2) DME > DMSO > DMF > THF > Benzene 
(3) DMSO > DMF > DME > THF > Benzene 
(4) Benzene > THF > DME > DMF > DMSO 
 
Q.19 Which one of the following is the major 
product of the hydrogenation reaction given 
below? (ANS.1) 
 
 
Q.20 Which one of the following features 
properties does glucose acquire through 
intramolecularhemiacetal formation? 
(1) Ability to form anhydride linkage with non-
carbohydrate moieties such as the 
inorganicphosphate 
(2) Ability to form epimers 
(3) An additional chiral carbon 
(4) Ability to function as a reducing agent 
 
Q.21 Presence of which one of the following 
in the urine indicates pregnancy in human? 
(1) Progesterone 
(2) Human chorionic gonadotropin 
(3) Estrogen 
(4)Follicle-stimulating hormone and 
luteinizinghormone 
 
Q.22 In plants, the ovules are attached to the 
ovary by 
(1) Embryo sac 
(2)Synergids 
(3) Placenta 
(4) Tube cells 
 
Q.23 Which one of the following isomers is 
thermodynamically most stable? (ANS.1) 
 
 
Q.24 Which one of the following microscopic 
techniques provides a 3-dimensional 
perspective of live,unstained and transparent 
specimens obtained from the wild? 
(1) Phase contrast microscopy 
(2) Confocal microscopy  
(3) Differential interference contrast 
(Nomarski)microscopy 
(4) Fluorescence microscopy 
 
Q.25 IUPAC name of the following molecule is 
 
(1) 3-Bromo-3,5-dimethyl hexane 
(2) 3-Bromo-2-isobutyl butane 
(3)4-Bromo-2,4-dimethyl hexane 
(4) 4-Bromo-2-methyl-4-ethyl pentane 
 
Q.26 The lack of linear correlation between 
the genome sizes and genetic complexities 
among variousspecies is known as 
(1) Genetic diversity 
(2) Central dogma 
(3) G-value paradox 
(4) C-value paradox 
 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.27 In a genetic cross between plants 
bearing violet flowers and green seeds 
(VVGG), and white flower and yellow seeds 
(rygg), thefollowing phenotypic distribution 
was obtained in the F2 progeny(assume both 
parents to be pure breeding for both the 
traits, and self-cross at F1 generation):  
i) 2340 plants with violet flowers and green 
seeds 
ii) 47 plants with violet flowers and yellow 
seeds 
iii) 43 plants with white flowers and green 
seeds 
iv) 770 plants with white flowers and yellow 
seeds 
Which one of the following interpretations 
explains the above phenotypic distribution? 
(1) Flower color in this plant species is a 
polygenic trait 
(2) Genes for flower and seed colors are 
present on the same chromosome 
(3) Same genes control both flower and seed 
colors 
(4) Genes for flower and seed colors are 
genetically interacting 
 
Q.28 Which one of the following represents 
the motion of an object with a positive 
acceleration?(ANS.4) 
 
 
 
 
 
 
 
Q.29 The difference between mitosis and 
meiosis I is 
(1) The DNA is double helical in meiosis I but 
not in mitosis 
(2) Sister chromatids separate in mitosis, 
whereas homologous chromosomes separate 
in meiosis I 
(3) Unlike in mitotic metaphase, chromosomes 
do not align at the equatorial plate in meiosis I 
(4)The nuclear membrane is absent during 
mitotic metaphase, but not in meiotic 
metaphase 
 
Q.30 If ?? +
?? ?? =?? , then the value of ?? ?? +
?? ?? ?? is 
(1)-2 
(2) 2 
(3) 1 
(4)-1 
 
B Section 
Q.1Which of the following is/are TRUE about 
the electron carrier, ubiquinone (coenzyme 
Q)? 
(1)Its hydrophilic nature and high affinity for 
protons enable ubiquinone to transport 
protonsreadily within the intermembrane 
space of mitochondria 
(2)Its ability to accept two electrons, one at a 
time, enables ubiquinone to function at the 
junctionbetween a 2-electron donor and a 1-
electron acceptor 
(3)Being small and hydrophobic, ubiquinone 
readily shuttles between protein-
basedelectrontransfer complexes within the 
membrane 
(4)Its ability to interact with Heme of 
cytochromes enables electron transport in 
themitochondrial membrane 
 
 
 
 
 
 
 
 
IIT JAM BIOTECHNOLOGY 2021  
Q.2 Infrared (IR) spectroscopy is used for 
determining certain aspects of the structure 
of organiccompounds. Which of the following 
statement(s) is/are FALSE? 
(1)Most organic functional groups absorb in a 
characteristic region of the IR spectrum 
(2) Each element absorbs at a characteristic 
wavelength 
(3) IR peak intensities are related to molecular 
mass 
(4) IR radiation induces electronic transitions 
 
Q.3Oleic acid, shown below, is 
 
(1) Insoluble in water 
(2) An unsaturated fatty acid 
(3) A saturated fatty acid 
(4) Soluble in acetone 
 
Q.4 Cyclic AMP (cAMP) acts as a second 
messenger for which of the following primary 
signalingmolecule(s)? 
(1) Prostaglandins 
(2) Retinoic acid 
(3) Epinephrine 
(4) Cortisol 
 
Q.5 which of the following molecular genetic 
technique(s) is/are used in forensic science? 
(1) DNA fingerprinting 
(2) Electrophoretic mobility shift assay 
(3) Coimmunoprecipitation 
(4)Restriction fragment length polymorphism 
 
Q.6 A function ??:???R is defined as ?? (?? )=
?? ?? +?? ?? ?? +??+?? , where ???R is the domain. The 
domain(s) on which the function ??(??) is one 
to one is/are 
(1) Rational numbers 
(2) Irrational numbers 
(3) Integers 
(4) Natural numbers 
 
 
 
Q.7 Which of the following pairs is/are 
analogous structures? 
(1) Dolphin flippers and fish fins 
(2) Bat wings and bird wings 
(3) Butterfly wings and bat wings 
(4) Human hands and bat wings 
 
Q.8 Which of the following is are common to 
both prokaryotic and eukaryotic gene 
expression? 
(1) Presence of the sequence TATA in the 
promoter 
(2) Genetic code 
(3) Post-translational modification 
(4) Coupled transcription and translation 
 
Q.9 A charged particle accelerated by a 
potential moves in a circular path with a 
velocity vin a uniform magnetic field B that is 
perpendicular to the motion. Which of the 
following is/are correct if the value of Vis 
increased 
(1) Time period of the motion increases 
(2) Radius of the circular path increases 
(3) Work done by the magnetic field increases 
(4) Kinetic energy of the particle increases 
 
Q.10 Which of the following is/are involved in 
the initiation of DNA replication? 
(1)oriC 
(2)RhoA 
(3)DnaA 
(4) Sigma factor 
 
Section: C 
 
Q.1 1.45 g of sucrose (C 12H 22O 11) is dissolved 
in 30.0 ml of water. Molality (rounded off to 3 
decimals) of the resulting solution is 
__________ m. 
ANS: 0.141 
 
Q.2 The number of triplet codon(s) for 
methionine is ___________. 
ANS: 1 
 
 
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