Page 1
Microbodies
Institute of Lifelong Learning, University of Delhi
Lesson: Microbodies
Lesson Developer: Geetika Kalra
College/Department:ANDC, University of Delhi
Page 2
Microbodies
Institute of Lifelong Learning, University of Delhi
Lesson: Microbodies
Lesson Developer: Geetika Kalra
College/Department:ANDC, University of Delhi
Microbodies
Institute of Lifelong Learning, University of Delhi 1
Table of Contents
Chapter: Microbodies
? Introduction
? Peroxisomes
? Structure
? Functions
? Fatty acid ß-oxidation
? Role of peroxisome in photorespiration
? Conversion of fixed nitrogen to nitrogen-rich
organic compounds
? Other functions
? Biogenesis
? Peroxisome assembly
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Page 3
Microbodies
Institute of Lifelong Learning, University of Delhi
Lesson: Microbodies
Lesson Developer: Geetika Kalra
College/Department:ANDC, University of Delhi
Microbodies
Institute of Lifelong Learning, University of Delhi 1
Table of Contents
Chapter: Microbodies
? Introduction
? Peroxisomes
? Structure
? Functions
? Fatty acid ß-oxidation
? Role of peroxisome in photorespiration
? Conversion of fixed nitrogen to nitrogen-rich
organic compounds
? Other functions
? Biogenesis
? Peroxisome assembly
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Microbodies
Institute of Lifelong Learning, University of Delhi 2
Introduction
Plant cells, protozoan cells, liver and kidney cells of vertebrates contain structurally simple
and functionally diverse organelles called Microbodies. They were first reported at
ultrastructural level in the proximal convoluted tubule of mouse kidney by Rhodin in 1954
and by Rouiller and Bernhard in 1956 in hepatic parenchymal cells. In plants these
organelles were first reported by Porter and Caulfield in 1958. Christian Rene
´
de Duve did
pioneering work in the discovery and isolation of these subcellular organelles. De Duve
separated these organelles on the basis of their sedimentation and density properties. The
1974 Nobel Prize for Physiology and Medicine was awarded to De Duve with Albert Claude
and George Palade for this work.
The microbodies are composed of single membrane that surrounds the finely granular
matrix. These organelles are home to diverse enzymatic reactions including several
metabolic reactions that provide energy. They can be distinguished from other organelles by
their richness in enzyme catalase. Their average diameter ranges from 0.1µm to 1.5µm.
Two types of microbodies have been distinguished :
? Peroxisomes –the peroxisomes are found in almost all eukaryotic cells and contain
enzymes that oxidize molecules like fatty acids and amino acids. The byproduct of
these oxidation reactions is hydrogen peroxide, which is converted to water and
oxygen by an enzyme catalase present in the peroxisomes.
? Glyoxysomes – contain in addition to the enzymes found in peroxisomes the
enzymes isocitrate lyase and/or malate synthetase the two enzymes of the
glyoxylate cycle. In germinating seeds these organelles are involved in mobilization
of fats.
Peroxisomes- Structure
Peroxisomes are ubiquitous single membrane (typical lipid bilayer) bound organelles that
contain various metabolic enzymes including those involved in energy metabolism.
Peroxisomes assume various forms. In rat liver these form large spheres of about 0.5 µm
diameter with a paracrystalline core while in fibroblasts it consists of small 0.1-0.2 µm
vesicles. Under some conditions in yeast cells and in liver cells these assume a tubular form,
which is interconnected to the spherical elements. In 1965, Christian de Duve, while
studying microbodies of rat liver, showed the presence of oxidases that transfer hydrogen
Page 4
Microbodies
Institute of Lifelong Learning, University of Delhi
Lesson: Microbodies
Lesson Developer: Geetika Kalra
College/Department:ANDC, University of Delhi
Microbodies
Institute of Lifelong Learning, University of Delhi 1
Table of Contents
Chapter: Microbodies
? Introduction
? Peroxisomes
? Structure
? Functions
? Fatty acid ß-oxidation
? Role of peroxisome in photorespiration
? Conversion of fixed nitrogen to nitrogen-rich
organic compounds
? Other functions
? Biogenesis
? Peroxisome assembly
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Microbodies
Institute of Lifelong Learning, University of Delhi 2
Introduction
Plant cells, protozoan cells, liver and kidney cells of vertebrates contain structurally simple
and functionally diverse organelles called Microbodies. They were first reported at
ultrastructural level in the proximal convoluted tubule of mouse kidney by Rhodin in 1954
and by Rouiller and Bernhard in 1956 in hepatic parenchymal cells. In plants these
organelles were first reported by Porter and Caulfield in 1958. Christian Rene
´
de Duve did
pioneering work in the discovery and isolation of these subcellular organelles. De Duve
separated these organelles on the basis of their sedimentation and density properties. The
1974 Nobel Prize for Physiology and Medicine was awarded to De Duve with Albert Claude
and George Palade for this work.
The microbodies are composed of single membrane that surrounds the finely granular
matrix. These organelles are home to diverse enzymatic reactions including several
metabolic reactions that provide energy. They can be distinguished from other organelles by
their richness in enzyme catalase. Their average diameter ranges from 0.1µm to 1.5µm.
Two types of microbodies have been distinguished :
? Peroxisomes –the peroxisomes are found in almost all eukaryotic cells and contain
enzymes that oxidize molecules like fatty acids and amino acids. The byproduct of
these oxidation reactions is hydrogen peroxide, which is converted to water and
oxygen by an enzyme catalase present in the peroxisomes.
? Glyoxysomes – contain in addition to the enzymes found in peroxisomes the
enzymes isocitrate lyase and/or malate synthetase the two enzymes of the
glyoxylate cycle. In germinating seeds these organelles are involved in mobilization
of fats.
Peroxisomes- Structure
Peroxisomes are ubiquitous single membrane (typical lipid bilayer) bound organelles that
contain various metabolic enzymes including those involved in energy metabolism.
Peroxisomes assume various forms. In rat liver these form large spheres of about 0.5 µm
diameter with a paracrystalline core while in fibroblasts it consists of small 0.1-0.2 µm
vesicles. Under some conditions in yeast cells and in liver cells these assume a tubular form,
which is interconnected to the spherical elements. In 1965, Christian de Duve, while
studying microbodies of rat liver, showed the presence of oxidases that transfer hydrogen
Microbodies
Institute of Lifelong Learning, University of Delhi 3
atom to molecular oxygen forming hydrogen peroxide. He coined the term peroxisomes, for
the organelle because it produced and consumed hydrogen peroxide. It occurs in some
animal cells and all photosynthetic cells of higher plants. In plants they perform wide range
of functions like participation in lipid mobilization, metabolism of free oxygen radicals,
synthesis of cholesterol and other lipids, catabolism of long chain fatty acids or conversion
of fixed nitrogen into nitrogen-rich organic compounds and many others.
Peroxisomes appear circular in cross section, have a single membrane enclosing a granular
matrix. These organelles do not have their own genome and all their proteins called
peroxins are encoded by the nuclear genome. These are synthesized on free ribosomes in
the cytosol and imported into the peroxisomes. Peroxisomes can replicate by division and
can also be regenerated de novo even if entirely lost from the cell.
Figure: The core of the peroxisomes appears as crystalline, crystalloid or multilamelllated.
Source: Duve, C.D. and Baudhun,P. 1966. Physiological reviews 46:303.
Page 5
Microbodies
Institute of Lifelong Learning, University of Delhi
Lesson: Microbodies
Lesson Developer: Geetika Kalra
College/Department:ANDC, University of Delhi
Microbodies
Institute of Lifelong Learning, University of Delhi 1
Table of Contents
Chapter: Microbodies
? Introduction
? Peroxisomes
? Structure
? Functions
? Fatty acid ß-oxidation
? Role of peroxisome in photorespiration
? Conversion of fixed nitrogen to nitrogen-rich
organic compounds
? Other functions
? Biogenesis
? Peroxisome assembly
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Microbodies
Institute of Lifelong Learning, University of Delhi 2
Introduction
Plant cells, protozoan cells, liver and kidney cells of vertebrates contain structurally simple
and functionally diverse organelles called Microbodies. They were first reported at
ultrastructural level in the proximal convoluted tubule of mouse kidney by Rhodin in 1954
and by Rouiller and Bernhard in 1956 in hepatic parenchymal cells. In plants these
organelles were first reported by Porter and Caulfield in 1958. Christian Rene
´
de Duve did
pioneering work in the discovery and isolation of these subcellular organelles. De Duve
separated these organelles on the basis of their sedimentation and density properties. The
1974 Nobel Prize for Physiology and Medicine was awarded to De Duve with Albert Claude
and George Palade for this work.
The microbodies are composed of single membrane that surrounds the finely granular
matrix. These organelles are home to diverse enzymatic reactions including several
metabolic reactions that provide energy. They can be distinguished from other organelles by
their richness in enzyme catalase. Their average diameter ranges from 0.1µm to 1.5µm.
Two types of microbodies have been distinguished :
? Peroxisomes –the peroxisomes are found in almost all eukaryotic cells and contain
enzymes that oxidize molecules like fatty acids and amino acids. The byproduct of
these oxidation reactions is hydrogen peroxide, which is converted to water and
oxygen by an enzyme catalase present in the peroxisomes.
? Glyoxysomes – contain in addition to the enzymes found in peroxisomes the
enzymes isocitrate lyase and/or malate synthetase the two enzymes of the
glyoxylate cycle. In germinating seeds these organelles are involved in mobilization
of fats.
Peroxisomes- Structure
Peroxisomes are ubiquitous single membrane (typical lipid bilayer) bound organelles that
contain various metabolic enzymes including those involved in energy metabolism.
Peroxisomes assume various forms. In rat liver these form large spheres of about 0.5 µm
diameter with a paracrystalline core while in fibroblasts it consists of small 0.1-0.2 µm
vesicles. Under some conditions in yeast cells and in liver cells these assume a tubular form,
which is interconnected to the spherical elements. In 1965, Christian de Duve, while
studying microbodies of rat liver, showed the presence of oxidases that transfer hydrogen
Microbodies
Institute of Lifelong Learning, University of Delhi 3
atom to molecular oxygen forming hydrogen peroxide. He coined the term peroxisomes, for
the organelle because it produced and consumed hydrogen peroxide. It occurs in some
animal cells and all photosynthetic cells of higher plants. In plants they perform wide range
of functions like participation in lipid mobilization, metabolism of free oxygen radicals,
synthesis of cholesterol and other lipids, catabolism of long chain fatty acids or conversion
of fixed nitrogen into nitrogen-rich organic compounds and many others.
Peroxisomes appear circular in cross section, have a single membrane enclosing a granular
matrix. These organelles do not have their own genome and all their proteins called
peroxins are encoded by the nuclear genome. These are synthesized on free ribosomes in
the cytosol and imported into the peroxisomes. Peroxisomes can replicate by division and
can also be regenerated de novo even if entirely lost from the cell.
Figure: The core of the peroxisomes appears as crystalline, crystalloid or multilamelllated.
Source: Duve, C.D. and Baudhun,P. 1966. Physiological reviews 46:303.
Microbodies
Institute of Lifelong Learning, University of Delhi 4
Figure: Structure of peroxisomes
Source:http://upload.wikimedia.org/wikipedia/commons/thumb/c/cb/Peroxisome.svg/300px
-Peroxisome.svg.png
DID YOU KNOW?
? Transport of proteins into peroxisomes is facilitated by a specific sequence of three
amino acids located at the carboxyl terminus of these proteins.
? Interestingly, if this sequence of amino acids is attached to a cytosolic protein, the
protein gets transported into peroxisomes.
? If this sequence is lacking in a human being – a disease called Zellweger syndrome
occurs which leads to severe protein deficiency in peroxisomes. Such patients suffer
from severe abnormalities in brain, liver and kidney and die soon after birth.
Functions
Peroxisomes contain 50 different enzymes involved in a variety of biochemical pathways.
Although initially identified in organelles that carried out oxidation reactions leading to the
production and eventually destruction of hydrogen peroxide. Peroxisomes contain the
enzymes – oxidases and catalases. The oxidases oxidizes the substrates ( RH
2
) and reduces
oxygen to hydrogen peroxide (H
2
O
2
). The hydrogen peroxide is decomposed by catalase
either by conversion to water (I) or by oxidation of another organic compound - R’H
2
(II).
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