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In any lab, a student performs a chymotrypsin digestion of the protein sequence
N-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.
What will be the result?
N-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.
What will be the result?
- a)No digestion occurs by chymotrypsin
- b)Because of the Pro after Phe, digestion does not occur
- c)Three fragments are generated
- d)2 fragments are generated
Correct answer is option 'A,B'. Can you explain this answer?
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Verified Answer
In any lab, a student performs a chymotrypsin digestion of the protein...
Chymotrypsin cleaves at C terminal of aromatic amino acids but these amino acids should not be followed by proline.
Most Upvoted Answer
In any lab, a student performs a chymotrypsin digestion of the protein...
Answer:
Chymotrypsin is a proteolytic enzyme that cleaves peptide bonds in a specific manner. It recognizes and cleaves peptide bonds adjacent to large hydrophobic amino acids such as phenylalanine, tyrosine, and tryptophan. Based on the given protein sequence, N-Lys-Arg-Phe-Pro-Glu-Tyr-Pro-Arg-Lys-Phe-C, the chymotrypsin digestion will result in the following:
No digestion occurs by chymotrypsin:
Chymotrypsin requires specific amino acids like phenylalanine, tyrosine, or tryptophan to cleave peptide bonds. In the given protein sequence, there are no phenylalanine, tyrosine, or tryptophan residues. Therefore, chymotrypsin cannot cleave any peptide bonds, resulting in no digestion.
Because of the Pro after Phe, digestion does not occur:
Chymotrypsin cleaves peptide bonds adjacent to large hydrophobic amino acids. However, in the given protein sequence, there is a proline (Pro) residue immediately after the phenylalanine (Phe) residue. Proline has a unique structure that restricts the rotation around the peptide bond and makes it difficult for chymotrypsin to access the peptide bond for cleavage. Therefore, digestion does not occur after the Phe residue.
Three fragments are generated:
Since no digestion occurs by chymotrypsin, there will be no fragments generated.
2 fragments are generated:
Since no digestion occurs by chymotrypsin, there will be no fragments generated.
Summary:
Based on the given protein sequence N-Lys-Arg-Phe-Pro-Glu-Tyr-Pro-Arg-Lys-Phe-C, both options A and B are correct. No digestion occurs by chymotrypsin due to the absence of phenylalanine, tyrosine, or tryptophan residues, as well as the presence of proline after the phenylalanine residue.
Chymotrypsin is a proteolytic enzyme that cleaves peptide bonds in a specific manner. It recognizes and cleaves peptide bonds adjacent to large hydrophobic amino acids such as phenylalanine, tyrosine, and tryptophan. Based on the given protein sequence, N-Lys-Arg-Phe-Pro-Glu-Tyr-Pro-Arg-Lys-Phe-C, the chymotrypsin digestion will result in the following:
No digestion occurs by chymotrypsin:
Chymotrypsin requires specific amino acids like phenylalanine, tyrosine, or tryptophan to cleave peptide bonds. In the given protein sequence, there are no phenylalanine, tyrosine, or tryptophan residues. Therefore, chymotrypsin cannot cleave any peptide bonds, resulting in no digestion.
Because of the Pro after Phe, digestion does not occur:
Chymotrypsin cleaves peptide bonds adjacent to large hydrophobic amino acids. However, in the given protein sequence, there is a proline (Pro) residue immediately after the phenylalanine (Phe) residue. Proline has a unique structure that restricts the rotation around the peptide bond and makes it difficult for chymotrypsin to access the peptide bond for cleavage. Therefore, digestion does not occur after the Phe residue.
Three fragments are generated:
Since no digestion occurs by chymotrypsin, there will be no fragments generated.
2 fragments are generated:
Since no digestion occurs by chymotrypsin, there will be no fragments generated.
Summary:
Based on the given protein sequence N-Lys-Arg-Phe-Pro-Glu-Tyr-Pro-Arg-Lys-Phe-C, both options A and B are correct. No digestion occurs by chymotrypsin due to the absence of phenylalanine, tyrosine, or tryptophan residues, as well as the presence of proline after the phenylalanine residue.
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In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer?
Question Description
In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer?.
In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer?.
Solutions for In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? in English & in Hindi are available as part of our courses for IIT JAM.
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In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer?, a detailed solution for In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? has been provided alongside types of In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice In any lab, a student performs a chymotrypsin digestion of the protein sequenceN-Lys-Arg-Phe-Pro-Glu-Tyro-Pro-Arg-Lys-Phe-C.What will be the result?a)No digestion occurs by chymotrypsinb)Because of the Pro after Phe, digestion does not occurc)Three fragments are generatedd)2 fragments are generatedCorrect answer is option 'A,B'. Can you explain this answer? tests, examples and also practice IIT JAM tests.
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