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In order to study the role of telomeres in DNA replication, genetically engineered mice were prepared. When the telomerase RNA gene was knocked out in mice, cells from these knocked out mice proliferated even after 100 cell divisions, which is quite likely in the case of human cells. Which of the following is the correct reason?
  • a)
     human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA  replication
  • b)
    In vitro, in mice, DNA  becomes circular due to end
    to
    end chromosome fusion and does not require replication.
  • c)
    Mice have very long stretch of telomere DNA sequence as compared to that of humans
  • d)
    In vitro mice DNA replication does not require the removal of RNA primers
Correct answer is option 'C'. Can you explain this answer?
Verified Answer
In order to study the role of telomeres in DNA replication, geneticall...
Concept:
  • Telomeres are ribonucleoprotein structures capping the end of every linear chromosome.
  • In all vertebrates, they are composed of TTAGGG repeats coated with specific protecting proteins.
  • Telomeres shorten with each mitotic cell division, but telomerase, a reverse transcriptase, elongate telomeres in very specific cells, such as embryonic and adult stem cells.
  • Although telomere sequence is identical in mice and humans and telomeres serve the same role of protecting chromosomes and genetic information from damage and erosion in both species, abnormalities in telomere maintenance and in telomerase function do not coincide in phenotype in humans and mice.
Explanation:
  • The telomeres of most laboratory mice are 5 to 10 times longer than in humans, but their lifespan is 30 times shorter.
  • Complete absence of telomerase has little expression in phenotype over several generations in mice, whereas heterozygosity for telomerase mutations in humans is sufficient to result in organ regeneration defect and cancer development.
  • Patients with telomerase deficiency and very short telomeres may develop aplastic anemia, pulmonary fibrosis, or cirrhosis, whereas telomerase-null murine models display only modest hematopoietic deficiency and develop emphysema when exposed to cigarette smoke.
  • In summary, telomerase deficiency in both humans and mice accelerate telomere shortening, but its consequences in the different organs and in the organism diverge, mainly due to telomere length differences.
Statement A: 
 human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA  replication
  • Consider the explanation above thus this option is not true, as 
    the human and mouse proteins are two of the largest members of the TERT family, at 127 kDa.
  • The TERT component of telomerase is a reverse transcriptase (RT), containing motifs (1 and 2, and A-E) that are common to RT enzymes, as well as N- and C-terminal regions which are not conserved among RT.
Statement B: 
In vitro, in mice, DNA  becomes circular due to end
to
end chromosome fusion and does not require replication.
  •  Consider the explanation above thus this option is not true, as DNA has telomeres end which repels the end of the chromosomes.
    ​​
Statement C: 
Mice have very long stretch of telomere DNA sequence as compared to that of humans
  • Consider the explanation above thus this option is  true, as the telomeres of most laboratory mice are 5 to 10 times longer than in humans.
​​
Statement D: 
In vitro mice DNA replication does not require the removal of RNA primers.
  • this statement is not true as replication invitro is also propagated by DNA polymerases.
                     
  hence the correct answer is option 3

 
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In order to study the role of telomeres in DNA replication, genetically engineered mice were prepared. When the telomerase RNA gene was knocked out in mice, cells from these knocked out mice proliferated even after 100 cell divisions, which is quite likely in the case of human cells. Which of the following is the correct reason?a)human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA replicationb)In vitro, in mice, DNA becomes circular due to end to end chromosome fusion and does not require replication.c)Mice have very long stretch of telomereDNA sequence as compared to that of humansd)In vitro mice DNA replication does not require the removal of RNA primersCorrect answer is option 'C'. Can you explain this answer?
Question Description
In order to study the role of telomeres in DNA replication, genetically engineered mice were prepared. When the telomerase RNA gene was knocked out in mice, cells from these knocked out mice proliferated even after 100 cell divisions, which is quite likely in the case of human cells. Which of the following is the correct reason?a)human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA replicationb)In vitro, in mice, DNA becomes circular due to end to end chromosome fusion and does not require replication.c)Mice have very long stretch of telomereDNA sequence as compared to that of humansd)In vitro mice DNA replication does not require the removal of RNA primersCorrect answer is option 'C'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about In order to study the role of telomeres in DNA replication, genetically engineered mice were prepared. When the telomerase RNA gene was knocked out in mice, cells from these knocked out mice proliferated even after 100 cell divisions, which is quite likely in the case of human cells. Which of the following is the correct reason?a)human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA replicationb)In vitro, in mice, DNA becomes circular due to end to end chromosome fusion and does not require replication.c)Mice have very long stretch of telomereDNA sequence as compared to that of humansd)In vitro mice DNA replication does not require the removal of RNA primersCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In order to study the role of telomeres in DNA replication, genetically engineered mice were prepared. When the telomerase RNA gene was knocked out in mice, cells from these knocked out mice proliferated even after 100 cell divisions, which is quite likely in the case of human cells. Which of the following is the correct reason?a)human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA replicationb)In vitro, in mice, DNA becomes circular due to end to end chromosome fusion and does not require replication.c)Mice have very long stretch of telomereDNA sequence as compared to that of humansd)In vitro mice DNA replication does not require the removal of RNA primersCorrect answer is option 'C'. Can you explain this answer?.
Solutions for In order to study the role of telomeres in DNA replication, genetically engineered mice were prepared. When the telomerase RNA gene was knocked out in mice, cells from these knocked out mice proliferated even after 100 cell divisions, which is quite likely in the case of human cells. Which of the following is the correct reason?a)human and mice are fundamentally different with respect to their requirements for telomerase enzyme in the context of DNA replicationb)In vitro, in mice, DNA becomes circular due to end to end chromosome fusion and does not require replication.c)Mice have very long stretch of telomereDNA sequence as compared to that of humansd)In vitro mice DNA replication does not require the removal of RNA primersCorrect answer is option 'C'. Can you explain this answer? in English & in Hindi are available as part of our courses for Software Development. Download more important topics, notes, lectures and mock test series for Software Development Exam by signing up for free.
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