Biotechnology and Genetic Engineering, PPT (Powerpoint Presentation), Semester, Engineering Notes | EduRev

Biotechnology Engineering (BT) : Biotechnology and Genetic Engineering, PPT (Powerpoint Presentation), Semester, Engineering Notes | EduRev

 Page 1


Biotechnology and Genetic Engineering 
PBIO 4500/5500 
•Gene libraries 
•cDNA libraries 
•Library screening 
 
Page 2


Biotechnology and Genetic Engineering 
PBIO 4500/5500 
•Gene libraries 
•cDNA libraries 
•Library screening 
 
Eukaryotic gene organization 
 
enhancers 
silencers 
Page 3


Biotechnology and Genetic Engineering 
PBIO 4500/5500 
•Gene libraries 
•cDNA libraries 
•Library screening 
 
Eukaryotic gene organization 
 
enhancers 
silencers 
Genomic 
library 
construction 
Page 4


Biotechnology and Genetic Engineering 
PBIO 4500/5500 
•Gene libraries 
•cDNA libraries 
•Library screening 
 
Eukaryotic gene organization 
 
enhancers 
silencers 
Genomic 
library 
construction 
Screening a genomic 
library using DNA 
hybridization to a 
(radio-)labeled DNA 
probe 
 
 Note: a cDNA is commonly 
(radio-)labeled and used as 
a DNA probe to screen a 
genomic library 
Page 5


Biotechnology and Genetic Engineering 
PBIO 4500/5500 
•Gene libraries 
•cDNA libraries 
•Library screening 
 
Eukaryotic gene organization 
 
enhancers 
silencers 
Genomic 
library 
construction 
Screening a genomic 
library using DNA 
hybridization to a 
(radio-)labeled DNA 
probe 
 
 Note: a cDNA is commonly 
(radio-)labeled and used as 
a DNA probe to screen a 
genomic library 
Production of a (radio-)labeled DNA probe by the random primer 
method [uses the Klenow fragment of DNA polymerase] 
5 ’ 
5 ’ 
5 ’ 
3 ’ 
3 ’ 
3 ’ 
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