Enzymes Regulation- PPT, IBT, Biotechnology, Engineering, Semester Notes | EduRev

: Enzymes Regulation- PPT, IBT, Biotechnology, Engineering, Semester Notes | EduRev

 Page 1


Chapter 5 (part 4)
Enzyme Regulation
Page 2


Chapter 5 (part 4)
Enzyme Regulation
Regulation of Enzyme Activity
Enzyme quantity – regulation of gene expression (Response time = 
minutes to hours)
a) Transcription
b) Translation
c) Enzyme turnover
Enzyme activity (rapid response time = fraction of seconds)
a) Allosteric regulation
b) Covalent modification
c) Association-disassociation’
d) Proteolytic cleavage of proenzyme
Page 3


Chapter 5 (part 4)
Enzyme Regulation
Regulation of Enzyme Activity
Enzyme quantity – regulation of gene expression (Response time = 
minutes to hours)
a) Transcription
b) Translation
c) Enzyme turnover
Enzyme activity (rapid response time = fraction of seconds)
a) Allosteric regulation
b) Covalent modification
c) Association-disassociation’
d) Proteolytic cleavage of proenzyme
Allosteric Regulation
• End products are often inhibitors
• Allosteric modulators bind to site other 
than the active site
• Allosteric enzymes usually have 4
o
structure
• Vo vs [S] plots give sigmoidal curve for 
at least one substrate
• Can remove allosteric site without 
effecting enzymatic action
Page 4


Chapter 5 (part 4)
Enzyme Regulation
Regulation of Enzyme Activity
Enzyme quantity – regulation of gene expression (Response time = 
minutes to hours)
a) Transcription
b) Translation
c) Enzyme turnover
Enzyme activity (rapid response time = fraction of seconds)
a) Allosteric regulation
b) Covalent modification
c) Association-disassociation’
d) Proteolytic cleavage of proenzyme
Allosteric Regulation
• End products are often inhibitors
• Allosteric modulators bind to site other 
than the active site
• Allosteric enzymes usually have 4
o
structure
• Vo vs [S] plots give sigmoidal curve for 
at least one substrate
• Can remove allosteric site without 
effecting enzymatic action
Regulation of Enzyme Activity
(biochemical regulation) 
•1
st
committed step of a biosynthetic pathway or 
enzymes at pathway branch points often regulated 
by feedback inhibition.
• Efficient use of biosynthetic precursors and 
energy
BA C
1
3”
3’
2
EF G
4’
5’
HI J
4”
5”
X
X
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