Page 1
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 1
Lesson Prepared Under MHRD project “National Mission on
Education Through ICT”
Discipline: Botany
Paper: Plant Metabolism
National Coordinator: Prof. S.C. Bhatla
Lesson: Nitrogen metabolism
Lesson Developer: Dr. Sunita Yadav
Department/College: Department of Botany, Hansraj
College
Lesson Reviewer: Prof. S.C. Bhatla
Department/College: Department of Botany, University of
Delhi
Language Editor: Vinee Khanna
Department/College: Department of Genetics, University
of Delhi, South Campus
Lesson Editor: Dr. Rama Sisodia, Fellow in Botany ILLL
Page 2
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 1
Lesson Prepared Under MHRD project “National Mission on
Education Through ICT”
Discipline: Botany
Paper: Plant Metabolism
National Coordinator: Prof. S.C. Bhatla
Lesson: Nitrogen metabolism
Lesson Developer: Dr. Sunita Yadav
Department/College: Department of Botany, Hansraj
College
Lesson Reviewer: Prof. S.C. Bhatla
Department/College: Department of Botany, University of
Delhi
Language Editor: Vinee Khanna
Department/College: Department of Genetics, University
of Delhi, South Campus
Lesson Editor: Dr. Rama Sisodia, Fellow in Botany ILLL
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 2
Table of Contents
Nitrogen Metabolism
? Introduction
? Industrial Nitrogen Fixation
? Atmospheric Nitrogen Fixation
? Lightning
? Photochemical reactions
? Biological Nitrogen Fixation
? Historical aspects of nitrogen fixation
? Biological nitrogen fixation
? Asymbiotic nitrogen fixation
? Aerobic nitrogen fixers
? Facultative nitrogen fixers
? Anaerobic nitrogen fixers
? Symbiotic nitrogen fixation
? Nitrogen fixation requires anaerobic conditions
? Process of nodulation in legumes
? Nodulin genes
? Nodulation genes
? Leghemeglobin
? Nitrogenase enzyme complex
? General characteristics
? Structure
? Azoferredoxin
? Azomolybdoferredoxin
? Regulation of Nitrogenase
? Unassimilated ammonium or nitrate may be dangerous
? Nitrate assimilation
? Nitrate reductase
Page 3
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 1
Lesson Prepared Under MHRD project “National Mission on
Education Through ICT”
Discipline: Botany
Paper: Plant Metabolism
National Coordinator: Prof. S.C. Bhatla
Lesson: Nitrogen metabolism
Lesson Developer: Dr. Sunita Yadav
Department/College: Department of Botany, Hansraj
College
Lesson Reviewer: Prof. S.C. Bhatla
Department/College: Department of Botany, University of
Delhi
Language Editor: Vinee Khanna
Department/College: Department of Genetics, University
of Delhi, South Campus
Lesson Editor: Dr. Rama Sisodia, Fellow in Botany ILLL
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 2
Table of Contents
Nitrogen Metabolism
? Introduction
? Industrial Nitrogen Fixation
? Atmospheric Nitrogen Fixation
? Lightning
? Photochemical reactions
? Biological Nitrogen Fixation
? Historical aspects of nitrogen fixation
? Biological nitrogen fixation
? Asymbiotic nitrogen fixation
? Aerobic nitrogen fixers
? Facultative nitrogen fixers
? Anaerobic nitrogen fixers
? Symbiotic nitrogen fixation
? Nitrogen fixation requires anaerobic conditions
? Process of nodulation in legumes
? Nodulin genes
? Nodulation genes
? Leghemeglobin
? Nitrogenase enzyme complex
? General characteristics
? Structure
? Azoferredoxin
? Azomolybdoferredoxin
? Regulation of Nitrogenase
? Unassimilated ammonium or nitrate may be dangerous
? Nitrate assimilation
? Nitrate reductase
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 3
? Structure
? Regulation
? Nitrite reductase
? Ammonium assimilation
? Introduction
? Two alternative pathways
? Reductive amination by GDH
? Ammonia incorporation by GS
? GOGAT or glutamate synthase
? NADH-GOGAT
? Fd-GOGAT
? Transamination reactions
? Aminotransferases
? Amino acid biosynthesis
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Page 4
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 1
Lesson Prepared Under MHRD project “National Mission on
Education Through ICT”
Discipline: Botany
Paper: Plant Metabolism
National Coordinator: Prof. S.C. Bhatla
Lesson: Nitrogen metabolism
Lesson Developer: Dr. Sunita Yadav
Department/College: Department of Botany, Hansraj
College
Lesson Reviewer: Prof. S.C. Bhatla
Department/College: Department of Botany, University of
Delhi
Language Editor: Vinee Khanna
Department/College: Department of Genetics, University
of Delhi, South Campus
Lesson Editor: Dr. Rama Sisodia, Fellow in Botany ILLL
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 2
Table of Contents
Nitrogen Metabolism
? Introduction
? Industrial Nitrogen Fixation
? Atmospheric Nitrogen Fixation
? Lightning
? Photochemical reactions
? Biological Nitrogen Fixation
? Historical aspects of nitrogen fixation
? Biological nitrogen fixation
? Asymbiotic nitrogen fixation
? Aerobic nitrogen fixers
? Facultative nitrogen fixers
? Anaerobic nitrogen fixers
? Symbiotic nitrogen fixation
? Nitrogen fixation requires anaerobic conditions
? Process of nodulation in legumes
? Nodulin genes
? Nodulation genes
? Leghemeglobin
? Nitrogenase enzyme complex
? General characteristics
? Structure
? Azoferredoxin
? Azomolybdoferredoxin
? Regulation of Nitrogenase
? Unassimilated ammonium or nitrate may be dangerous
? Nitrate assimilation
? Nitrate reductase
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 3
? Structure
? Regulation
? Nitrite reductase
? Ammonium assimilation
? Introduction
? Two alternative pathways
? Reductive amination by GDH
? Ammonia incorporation by GS
? GOGAT or glutamate synthase
? NADH-GOGAT
? Fd-GOGAT
? Transamination reactions
? Aminotransferases
? Amino acid biosynthesis
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 4
Introduction
Nitrogen is present in many forms in the biosphere. Although vast quantities of molecular
nitrogen is present in the atmosphere (77% by volume), it is not available for direct use by
living organisms. Acquisition of this molecular nitrogen and it’s conversion to ammonium
(NH
3
) and nitrate (NO
3
-
) accounts for nitrogen fixation. Conversion of inorganic to organic
nitrogen is carried out by plants mainly. Nucleotides and amino acids (found in nucleic acids
and proteins) mainly have nitrogen as their principal moiety constituting the prominent
biochemical compounds in plant cells.
The process of nitrogen fixation requires a large input of energy because it accounts to the
production of NH
3
and NO
3
-
from nitrogen molecule by breakage of the stable triple covalent
bond present in the dinitrogen molecule. Both natural and industrial processes contribute to
the process of nitrogen fixation.
1. Industrial Nitrogen Fixation: This type of fixation contributes to 20% of total
nitrogen fixed. Nitrogen in combination with hydrogen forms ammonia in the presence of
high temperature (about 200
?
C) and high pressure (about 200 atmospheres) and a metal
catalyst (like iron). High activation energy of such a condensation reaction is overcomed by
such extreme conditions i.e. high temperature and high pressure.
N
2
+ H
2
2 NH
3
Nitrogen Hydrogen Ammonia
This reaction is called Haber-Bosch process and serves as an initial trigger for the
production of industrial and agricultural goods, as illustrated in the following equation:
O
2 NH
3
+ CO
2
H
2
N-CO-NH
2
Ammonia Carbon dioxide Urea
2. Atmospheric nitrogen fixation: This can occur by any of the following two
methods:
i) Lightning: This form of nitrogen fixation amounts to about 8% of the total nitrogen fixed.
In the presence of lightning, highly reactive OH
?
, H and O are formed from water vapour
Page 5
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 1
Lesson Prepared Under MHRD project “National Mission on
Education Through ICT”
Discipline: Botany
Paper: Plant Metabolism
National Coordinator: Prof. S.C. Bhatla
Lesson: Nitrogen metabolism
Lesson Developer: Dr. Sunita Yadav
Department/College: Department of Botany, Hansraj
College
Lesson Reviewer: Prof. S.C. Bhatla
Department/College: Department of Botany, University of
Delhi
Language Editor: Vinee Khanna
Department/College: Department of Genetics, University
of Delhi, South Campus
Lesson Editor: Dr. Rama Sisodia, Fellow in Botany ILLL
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 2
Table of Contents
Nitrogen Metabolism
? Introduction
? Industrial Nitrogen Fixation
? Atmospheric Nitrogen Fixation
? Lightning
? Photochemical reactions
? Biological Nitrogen Fixation
? Historical aspects of nitrogen fixation
? Biological nitrogen fixation
? Asymbiotic nitrogen fixation
? Aerobic nitrogen fixers
? Facultative nitrogen fixers
? Anaerobic nitrogen fixers
? Symbiotic nitrogen fixation
? Nitrogen fixation requires anaerobic conditions
? Process of nodulation in legumes
? Nodulin genes
? Nodulation genes
? Leghemeglobin
? Nitrogenase enzyme complex
? General characteristics
? Structure
? Azoferredoxin
? Azomolybdoferredoxin
? Regulation of Nitrogenase
? Unassimilated ammonium or nitrate may be dangerous
? Nitrate assimilation
? Nitrate reductase
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 3
? Structure
? Regulation
? Nitrite reductase
? Ammonium assimilation
? Introduction
? Two alternative pathways
? Reductive amination by GDH
? Ammonia incorporation by GS
? GOGAT or glutamate synthase
? NADH-GOGAT
? Fd-GOGAT
? Transamination reactions
? Aminotransferases
? Amino acid biosynthesis
? Summary
? Exercise/ Practice
? Glossary
? References/ Bibliography/ Further Reading
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 4
Introduction
Nitrogen is present in many forms in the biosphere. Although vast quantities of molecular
nitrogen is present in the atmosphere (77% by volume), it is not available for direct use by
living organisms. Acquisition of this molecular nitrogen and it’s conversion to ammonium
(NH
3
) and nitrate (NO
3
-
) accounts for nitrogen fixation. Conversion of inorganic to organic
nitrogen is carried out by plants mainly. Nucleotides and amino acids (found in nucleic acids
and proteins) mainly have nitrogen as their principal moiety constituting the prominent
biochemical compounds in plant cells.
The process of nitrogen fixation requires a large input of energy because it accounts to the
production of NH
3
and NO
3
-
from nitrogen molecule by breakage of the stable triple covalent
bond present in the dinitrogen molecule. Both natural and industrial processes contribute to
the process of nitrogen fixation.
1. Industrial Nitrogen Fixation: This type of fixation contributes to 20% of total
nitrogen fixed. Nitrogen in combination with hydrogen forms ammonia in the presence of
high temperature (about 200
?
C) and high pressure (about 200 atmospheres) and a metal
catalyst (like iron). High activation energy of such a condensation reaction is overcomed by
such extreme conditions i.e. high temperature and high pressure.
N
2
+ H
2
2 NH
3
Nitrogen Hydrogen Ammonia
This reaction is called Haber-Bosch process and serves as an initial trigger for the
production of industrial and agricultural goods, as illustrated in the following equation:
O
2 NH
3
+ CO
2
H
2
N-CO-NH
2
Ammonia Carbon dioxide Urea
2. Atmospheric nitrogen fixation: This can occur by any of the following two
methods:
i) Lightning: This form of nitrogen fixation amounts to about 8% of the total nitrogen fixed.
In the presence of lightning, highly reactive OH
?
, H and O are formed from water vapour
Nitrogen Metabolism
Institute of Lifelong Learning, University of Delhi 5
and oxygen. These free H and O atoms subsequently form nitric acid (HNO
3
) when they
come in contact with N
2
. HNO
3
thus formed, falls along with rain on earth surface.
N
2
+ O
2
NO
Nitrogen Oxygen Nitric oxide
NO + O
2
NO
2
H
2
O + O
2
HNO
3
Nitric acid
Falls along with rain
ii) Photochemical reactions: This contributes to about 2% of the total nitrogen fixed. This
kind of reaction leads to the production of nitric acid that subsequently falls along with rain.
NO + O
3
HNO
3
Nitric oxide Ozone Nitric acid (falls along with rain)
3. Biological nitrogen fixation: This contributes to about 70% of the total nitrogen fixed.
This process is carried out mainly by prokaryotic microorganisms like bacteria or blue green
algae (cyanobacteria) which fix nitrogen into ammonia (NH
3
). This ammonia dissolves in
water to form ammonium:
NH
3
+ H
2
O NH
4
+
+ OH
-
Ammonia Water Ammonium
This process of nitrogen fixation is very critical from agricultural point of view because
nitrogen fertilizers produced through industrial means hardly ever meet agricultural
requirements.
This process can occur either asymbiotically or symbiotically which has been discussed in
detail later in the chapter.
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