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Page 1 Tissue Culture applications – Part I Lesson Prepared Under MHRD project “National Mission on Education Through ICT” Discipline: Botany Paper: Bioinformatics National Coordinator: Prof. S.C. Bhatla Lesson: Tissue Culture Applications- Part I Lesson Developer: Namrata Dhaka Department/College: Department of Genetics, University of Delhi South Campus Lesson Reviewer: Prof. S.C. Bhatla Department/College: Language Editor: Vinee Khanna Department/College: Department of Genetics, University of Delhi South Campus Lesson Editor: Dr Rama Sisodia, Fellow in Botany ILLL Page 2 Tissue Culture applications – Part I Lesson Prepared Under MHRD project “National Mission on Education Through ICT” Discipline: Botany Paper: Bioinformatics National Coordinator: Prof. S.C. Bhatla Lesson: Tissue Culture Applications- Part I Lesson Developer: Namrata Dhaka Department/College: Department of Genetics, University of Delhi South Campus Lesson Reviewer: Prof. S.C. Bhatla Department/College: Language Editor: Vinee Khanna Department/College: Department of Genetics, University of Delhi South Campus Lesson Editor: Dr Rama Sisodia, Fellow in Botany ILLL Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 2 Learning Outcomes The chapter is aimed at enabling the readers to learn: ? The basic principle behind tissue culture techniques of micropropagation, endosperm culture and virus elimination. ? Major discoveries that led to the development of these techniques. ? Different methods and applications of these techniques. ? The importance of tissue culture in context of both, scientific research and commercial applications. Page 3 Tissue Culture applications – Part I Lesson Prepared Under MHRD project “National Mission on Education Through ICT” Discipline: Botany Paper: Bioinformatics National Coordinator: Prof. S.C. Bhatla Lesson: Tissue Culture Applications- Part I Lesson Developer: Namrata Dhaka Department/College: Department of Genetics, University of Delhi South Campus Lesson Reviewer: Prof. S.C. Bhatla Department/College: Language Editor: Vinee Khanna Department/College: Department of Genetics, University of Delhi South Campus Lesson Editor: Dr Rama Sisodia, Fellow in Botany ILLL Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 2 Learning Outcomes The chapter is aimed at enabling the readers to learn: ? The basic principle behind tissue culture techniques of micropropagation, endosperm culture and virus elimination. ? Major discoveries that led to the development of these techniques. ? Different methods and applications of these techniques. ? The importance of tissue culture in context of both, scientific research and commercial applications. Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 3 Table of Contents Chapter: Tissue Culture Applications – Part I ? Introduction ? Micropropagation ? Principle ? Discovery ? Methodology ? Applications ? Virus Elimination ? Principle ? Discovery ? Methodology ? Applications ? Endosperm Culture ? Principle ? Discovery ? Methodology ? Applications ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Page 4 Tissue Culture applications – Part I Lesson Prepared Under MHRD project “National Mission on Education Through ICT” Discipline: Botany Paper: Bioinformatics National Coordinator: Prof. S.C. Bhatla Lesson: Tissue Culture Applications- Part I Lesson Developer: Namrata Dhaka Department/College: Department of Genetics, University of Delhi South Campus Lesson Reviewer: Prof. S.C. Bhatla Department/College: Language Editor: Vinee Khanna Department/College: Department of Genetics, University of Delhi South Campus Lesson Editor: Dr Rama Sisodia, Fellow in Botany ILLL Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 2 Learning Outcomes The chapter is aimed at enabling the readers to learn: ? The basic principle behind tissue culture techniques of micropropagation, endosperm culture and virus elimination. ? Major discoveries that led to the development of these techniques. ? Different methods and applications of these techniques. ? The importance of tissue culture in context of both, scientific research and commercial applications. Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 3 Table of Contents Chapter: Tissue Culture Applications – Part I ? Introduction ? Micropropagation ? Principle ? Discovery ? Methodology ? Applications ? Virus Elimination ? Principle ? Discovery ? Methodology ? Applications ? Endosperm Culture ? Principle ? Discovery ? Methodology ? Applications ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 4 Introduction As you would be well aware, there is much talk today about „Genetically Modified? crops or transgenic crops which are genetically transformed to express desirable traits. This was a remarkable feat in the field of biology. Do you know how many years of basic scientific and technological research it took to achieve this possibility of plant transformation? It took about 88 years. See the Table ahead to get a glimpse of the major discoveries that led to it. This feat was possible due to active research in three different areas, conducted in the early twentieth century: ? Tissue culture of plants, ? Plant regeneration from single somatic cells, ? Study of crown gall disease. In this Chapter, we shall focus on the applications of first of the above mentioned research areas i.e. „Plant Tissue Culture?. It is defined as a system in which cells satisfy two main requirements – (1) remain undifferentiated (2) capable of infinite growth (White, 1939a). We would mainly discuss how the different techniques of plant tissue culture have found applications in different spheres of Plant Biology. Page 5 Tissue Culture applications – Part I Lesson Prepared Under MHRD project “National Mission on Education Through ICT” Discipline: Botany Paper: Bioinformatics National Coordinator: Prof. S.C. Bhatla Lesson: Tissue Culture Applications- Part I Lesson Developer: Namrata Dhaka Department/College: Department of Genetics, University of Delhi South Campus Lesson Reviewer: Prof. S.C. Bhatla Department/College: Language Editor: Vinee Khanna Department/College: Department of Genetics, University of Delhi South Campus Lesson Editor: Dr Rama Sisodia, Fellow in Botany ILLL Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 2 Learning Outcomes The chapter is aimed at enabling the readers to learn: ? The basic principle behind tissue culture techniques of micropropagation, endosperm culture and virus elimination. ? Major discoveries that led to the development of these techniques. ? Different methods and applications of these techniques. ? The importance of tissue culture in context of both, scientific research and commercial applications. Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 3 Table of Contents Chapter: Tissue Culture Applications – Part I ? Introduction ? Micropropagation ? Principle ? Discovery ? Methodology ? Applications ? Virus Elimination ? Principle ? Discovery ? Methodology ? Applications ? Endosperm Culture ? Principle ? Discovery ? Methodology ? Applications ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 4 Introduction As you would be well aware, there is much talk today about „Genetically Modified? crops or transgenic crops which are genetically transformed to express desirable traits. This was a remarkable feat in the field of biology. Do you know how many years of basic scientific and technological research it took to achieve this possibility of plant transformation? It took about 88 years. See the Table ahead to get a glimpse of the major discoveries that led to it. This feat was possible due to active research in three different areas, conducted in the early twentieth century: ? Tissue culture of plants, ? Plant regeneration from single somatic cells, ? Study of crown gall disease. In this Chapter, we shall focus on the applications of first of the above mentioned research areas i.e. „Plant Tissue Culture?. It is defined as a system in which cells satisfy two main requirements – (1) remain undifferentiated (2) capable of infinite growth (White, 1939a). We would mainly discuss how the different techniques of plant tissue culture have found applications in different spheres of Plant Biology. Tissue Culture Applications- Part I Institute of Lifelong Learning, University of Delhi 5 Figure: Various applications of plant tissue culture. Developed by: Author Figure: Chronological achievements in Plant Biotechnology. Source: Sussex, Ian M. "The scientific roots of modern plant biotechnology." The Plant Cell Online 20.5 (2008): 1189-1198.Read More
1. What is tissue culture? |
2. What are the applications of tissue culture in botany? |
3. How is tissue culture used for the mass production of plants? |
4. Can tissue culture be used to produce disease-free plant material? |
5. How does tissue culture contribute to the preservation of endangered plant species? |
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