Nutrition - Notes, Micro-biology, Semester, Engineering, Biotechnology Notes | EduRev

: Nutrition - Notes, Micro-biology, Semester, Engineering, Biotechnology Notes | EduRev

 Page 1


Lecture 5 Nutrition/Transport Bio119 
1 
I. Nutrition 
A. Modes of growth (the “trophs”) 
1. Autotrophs 
2. Phototrophs 
3. Chemotrophs 
4. Combinations 
a) Chemoheterotrophs 
b) Photoautotrophs 
c) Chemoautotrophs 
5. Obligate and facultative (Box 5.2) 
B. What does a cell need to grow? 
1. First let's see what cells are made of: 
2. Composition of cells (Table 5.1) 
3. C, O, N, H, S, P 
 
 
 
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Page 2


Lecture 5 Nutrition/Transport Bio119 
1 
I. Nutrition 
A. Modes of growth (the “trophs”) 
1. Autotrophs 
2. Phototrophs 
3. Chemotrophs 
4. Combinations 
a) Chemoheterotrophs 
b) Photoautotrophs 
c) Chemoautotrophs 
5. Obligate and facultative (Box 5.2) 
B. What does a cell need to grow? 
1. First let's see what cells are made of: 
2. Composition of cells (Table 5.1) 
3. C, O, N, H, S, P 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
2 
4. Salts 
 
5. Trace elements 
 
 
 
6. Growth factors 
 
 
 
II. How do prokaryotes get their nutrients? 
A. By transporting it in 
 
 
 
 
 
 
 
 
B. Cytoplasmic membrane is selectively permeable 
 
 
 
 
 
 
 
 
 
 
 
 
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Page 3


Lecture 5 Nutrition/Transport Bio119 
1 
I. Nutrition 
A. Modes of growth (the “trophs”) 
1. Autotrophs 
2. Phototrophs 
3. Chemotrophs 
4. Combinations 
a) Chemoheterotrophs 
b) Photoautotrophs 
c) Chemoautotrophs 
5. Obligate and facultative (Box 5.2) 
B. What does a cell need to grow? 
1. First let's see what cells are made of: 
2. Composition of cells (Table 5.1) 
3. C, O, N, H, S, P 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
2 
4. Salts 
 
5. Trace elements 
 
 
 
6. Growth factors 
 
 
 
II. How do prokaryotes get their nutrients? 
A. By transporting it in 
 
 
 
 
 
 
 
 
B. Cytoplasmic membrane is selectively permeable 
 
 
 
 
 
 
 
 
 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
3 
C. Diffusion (Fig. 5.2) 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
D. The role of active transport 
 
 
 
 
 
 
 
 
 
1. Secondary transporters (Fig. 5.4) 
a) Uniporters 
 
b) Symporters (Fig. 5.5) 
 
c) Antiporters 
 
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Page 4


Lecture 5 Nutrition/Transport Bio119 
1 
I. Nutrition 
A. Modes of growth (the “trophs”) 
1. Autotrophs 
2. Phototrophs 
3. Chemotrophs 
4. Combinations 
a) Chemoheterotrophs 
b) Photoautotrophs 
c) Chemoautotrophs 
5. Obligate and facultative (Box 5.2) 
B. What does a cell need to grow? 
1. First let's see what cells are made of: 
2. Composition of cells (Table 5.1) 
3. C, O, N, H, S, P 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
2 
4. Salts 
 
5. Trace elements 
 
 
 
6. Growth factors 
 
 
 
II. How do prokaryotes get their nutrients? 
A. By transporting it in 
 
 
 
 
 
 
 
 
B. Cytoplasmic membrane is selectively permeable 
 
 
 
 
 
 
 
 
 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
3 
C. Diffusion (Fig. 5.2) 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
D. The role of active transport 
 
 
 
 
 
 
 
 
 
1. Secondary transporters (Fig. 5.4) 
a) Uniporters 
 
b) Symporters (Fig. 5.5) 
 
c) Antiporters 
 
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Lecture 5 Nutrition/Transport Bio119 
4 
2. Group translocation (Fig. 5.6) 
 
 
 
 
3. ABC Transporters (Fig. 5.3) 
 
 
 
 
a) Iron uptake example  (Fig. 5.8) 
 
 
 
 
 
 
 
 
 
 
 
 
III. How to grow bacteria: 
A. Most prokaryotes cannot be cultured in the lab 
 
 
 
B. Culture medium 
1. Complex 
 
 
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Page 5


Lecture 5 Nutrition/Transport Bio119 
1 
I. Nutrition 
A. Modes of growth (the “trophs”) 
1. Autotrophs 
2. Phototrophs 
3. Chemotrophs 
4. Combinations 
a) Chemoheterotrophs 
b) Photoautotrophs 
c) Chemoautotrophs 
5. Obligate and facultative (Box 5.2) 
B. What does a cell need to grow? 
1. First let's see what cells are made of: 
2. Composition of cells (Table 5.1) 
3. C, O, N, H, S, P 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
2 
4. Salts 
 
5. Trace elements 
 
 
 
6. Growth factors 
 
 
 
II. How do prokaryotes get their nutrients? 
A. By transporting it in 
 
 
 
 
 
 
 
 
B. Cytoplasmic membrane is selectively permeable 
 
 
 
 
 
 
 
 
 
 
 
 
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Lecture 5 Nutrition/Transport Bio119 
3 
C. Diffusion (Fig. 5.2) 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
D. The role of active transport 
 
 
 
 
 
 
 
 
 
1. Secondary transporters (Fig. 5.4) 
a) Uniporters 
 
b) Symporters (Fig. 5.5) 
 
c) Antiporters 
 
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Lecture 5 Nutrition/Transport Bio119 
4 
2. Group translocation (Fig. 5.6) 
 
 
 
 
3. ABC Transporters (Fig. 5.3) 
 
 
 
 
a) Iron uptake example  (Fig. 5.8) 
 
 
 
 
 
 
 
 
 
 
 
 
III. How to grow bacteria: 
A. Most prokaryotes cannot be cultured in the lab 
 
 
 
B. Culture medium 
1. Complex 
 
 
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Lecture 5 Nutrition/Transport Bio119 
5 
2. Defined (Table 5.2, what’s missing?) 
 
 
3. Carbon source 
 
4. Energy source 
 
 
5. Solid vs. liquid 
 
 
 
6. Sterilization methods 
 
 
 
 
 
 
 
7. Aseptic techniques 
 
 
 
 
 
 
C. Salinity, Acidity, and alkalinity 
1. Halophilic (Fig. 6.17) 
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