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1. What is chromatography?
Ans. Chromatography is a laboratory technique used to separate and analyze mixtures of substances into their individual components. It involves the separation of components based on their differential interactions with a stationary phase and a mobile phase.
2. How does chromatography work?
Ans. Chromatography works by passing a mixture through a stationary phase, which can be a solid or a liquid, and a mobile phase, which is typically a liquid or a gas. The different components of the mixture interact differently with the stationary phase, causing them to move at different rates and separate from one another.
3. What are the different types of chromatography?
Ans. There are several types of chromatography, including gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type has its own specific application and uses different principles of separation.
4. What are some applications of chromatography?
Ans. Chromatography has a wide range of applications in various industries. It is commonly used in pharmaceuticals to analyze drug purity, in environmental testing to detect pollutants, in forensic analysis to identify substances at crime scenes, and in food and beverage industries to ensure product quality and safety.
5. What are the advantages of using chromatography?
Ans. Chromatography offers several advantages, such as its ability to separate complex mixtures into individual components, its high sensitivity and accuracy in detecting and quantifying substances, and its versatility in analyzing a wide range of compounds. It is a widely used technique in research, quality control, and forensic analysis due to its reliability and effectiveness.
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