UV-Visible Spectrophotometery Notes | Study Inorganic Chemistry - Chemistry

Chemistry: UV-Visible Spectrophotometery Notes | Study Inorganic Chemistry - Chemistry

The document UV-Visible Spectrophotometery Notes | Study Inorganic Chemistry - Chemistry is a part of the Chemistry Course Inorganic Chemistry.
All you need of Chemistry at this link: Chemistry
Introduction

The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Spectroscopy is based on the interaction between light and matter. When the matter absorbs the light, it undergoes excitation and de-excitation, resulting in the production of a spectrum.
When matter absorbs ultraviolet radiation, the electrons present in it undergo excitation. This causes them to jump from a ground state (an energy state with a relatively small amount of energy associated with it) to an excited state (an energy state with a relatively large amount of energy associated with it). It is important to note that the difference in the energies of the ground state and the excited state of the electron is always equal to the amount of ultraviolet radiation or visible radiation absorbed by it.

UV-Visible Spectroscopy and the Beer-Lambert Law

The statement of the Beer-Lambert law can be written as follows: When a beam of monochromatic light is made incident on a solution that contains a substance that absorbs the monochromatic light, the rate at which the intensity of the beam decreases along the thickness of the solution is directly proportional to the concentration of the absorbing substance in the solution and is also directly proportional to the intensity of the incident monochromatic radiation.

As per the Beer-Lambert law, the greater the number of absorbing molecules (that have the ability to absorb light of a specific wavelength), the greater the extent of absorption of the radiation.
To learn more about the principle of UV-Visible spectroscopy and other related concepts such as infrared spectroscopy, register with BYJU’S and download the mobile application on your smartphone.

The document UV-Visible Spectrophotometery Notes | Study Inorganic Chemistry - Chemistry is a part of the Chemistry Course Inorganic Chemistry.
All you need of Chemistry at this link: Chemistry
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