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JEE Main Previous Year Questions (2025): Biomolecules

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 Page 1


T : 1 Q he total number of hydrogen bonds of a DNA-double Helix strand
whose one strand has the following sequence of bases is ________.
JEE Main 2025 (Online) 4th April Morning Shift
Ans: 33
Solution:
Two nucleic acid chains are wound about each other and held together by H bonds between pair of bases.
Adenine from two hydrogen bonds with thymine and Guanine form three hydrogen bond with cytosine.
In given DNA strand total seven guanine and cytosine bases which form total 21 H -bonds and six adenine
and thymine base which will form total 12 H -bonds with other DNA strand.
Total no. of H bonds 
Ans. 33
W : 3 2 Q hich of the following acids is a vitamin ?
JEE Main 2025 (Online) 22nd January Morning Shift
5 ' - G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' 5 ' G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' = 7 × 3 + 6 × 2 = 3 3
Page 2


T : 1 Q he total number of hydrogen bonds of a DNA-double Helix strand
whose one strand has the following sequence of bases is ________.
JEE Main 2025 (Online) 4th April Morning Shift
Ans: 33
Solution:
Two nucleic acid chains are wound about each other and held together by H bonds between pair of bases.
Adenine from two hydrogen bonds with thymine and Guanine form three hydrogen bond with cytosine.
In given DNA strand total seven guanine and cytosine bases which form total 21 H -bonds and six adenine
and thymine base which will form total 12 H -bonds with other DNA strand.
Total no. of H bonds 
Ans. 33
W : 3 2 Q hich of the following acids is a vitamin ?
JEE Main 2025 (Online) 22nd January Morning Shift
5 ' - G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' 5 ' G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' = 7 × 3 + 6 × 2 = 3 3 Options:
A. Saccharic acid
B. Ascorbic acid
C. Aspartic acid
D. Adipic acid
Ans: B
Solution:
Correct Answer: Ascorbic Acid
Ascorbic acid is commonly known as Vitamin C. It is a water-soluble vitamin essential for various bodily
functions, including:
Antioxidant Function: It helps protect cells from damage caused by free radicals.
Collagen Synthesis: It is crucial for the formation of collagen, a protein necessary for skin, cartilage,
tendons, ligaments, and blood vessels.
Immune Support: It supports the immune system by enhancing the body's natural defenses.
Iron Absorption: It improves the absorption of non-heme iron from plant-based foods.
Among the given options, only ascorbic acid qualifies as a vitamin.
I : 3 Q dentify the number of structure/s from the following which can be
corelated to D-glyceraldehyde.
JEE Main 2025 (Online) 22nd January Evening Shift
Page 3


T : 1 Q he total number of hydrogen bonds of a DNA-double Helix strand
whose one strand has the following sequence of bases is ________.
JEE Main 2025 (Online) 4th April Morning Shift
Ans: 33
Solution:
Two nucleic acid chains are wound about each other and held together by H bonds between pair of bases.
Adenine from two hydrogen bonds with thymine and Guanine form three hydrogen bond with cytosine.
In given DNA strand total seven guanine and cytosine bases which form total 21 H -bonds and six adenine
and thymine base which will form total 12 H -bonds with other DNA strand.
Total no. of H bonds 
Ans. 33
W : 3 2 Q hich of the following acids is a vitamin ?
JEE Main 2025 (Online) 22nd January Morning Shift
5 ' - G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' 5 ' G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' = 7 × 3 + 6 × 2 = 3 3 Options:
A. Saccharic acid
B. Ascorbic acid
C. Aspartic acid
D. Adipic acid
Ans: B
Solution:
Correct Answer: Ascorbic Acid
Ascorbic acid is commonly known as Vitamin C. It is a water-soluble vitamin essential for various bodily
functions, including:
Antioxidant Function: It helps protect cells from damage caused by free radicals.
Collagen Synthesis: It is crucial for the formation of collagen, a protein necessary for skin, cartilage,
tendons, ligaments, and blood vessels.
Immune Support: It supports the immune system by enhancing the body's natural defenses.
Iron Absorption: It improves the absorption of non-heme iron from plant-based foods.
Among the given options, only ascorbic acid qualifies as a vitamin.
I : 3 Q dentify the number of structure/s from the following which can be
corelated to D-glyceraldehyde.
JEE Main 2025 (Online) 22nd January Evening Shift
Options:
A. three
B. two
C. one
D. four
Ans: A
Solution:
In  group in right hand side then D-configuration is assigned. A , B , D - O H G : 4 Q iven below are two statements:
Page 4


T : 1 Q he total number of hydrogen bonds of a DNA-double Helix strand
whose one strand has the following sequence of bases is ________.
JEE Main 2025 (Online) 4th April Morning Shift
Ans: 33
Solution:
Two nucleic acid chains are wound about each other and held together by H bonds between pair of bases.
Adenine from two hydrogen bonds with thymine and Guanine form three hydrogen bond with cytosine.
In given DNA strand total seven guanine and cytosine bases which form total 21 H -bonds and six adenine
and thymine base which will form total 12 H -bonds with other DNA strand.
Total no. of H bonds 
Ans. 33
W : 3 2 Q hich of the following acids is a vitamin ?
JEE Main 2025 (Online) 22nd January Morning Shift
5 ' - G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' 5 ' G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' = 7 × 3 + 6 × 2 = 3 3 Options:
A. Saccharic acid
B. Ascorbic acid
C. Aspartic acid
D. Adipic acid
Ans: B
Solution:
Correct Answer: Ascorbic Acid
Ascorbic acid is commonly known as Vitamin C. It is a water-soluble vitamin essential for various bodily
functions, including:
Antioxidant Function: It helps protect cells from damage caused by free radicals.
Collagen Synthesis: It is crucial for the formation of collagen, a protein necessary for skin, cartilage,
tendons, ligaments, and blood vessels.
Immune Support: It supports the immune system by enhancing the body's natural defenses.
Iron Absorption: It improves the absorption of non-heme iron from plant-based foods.
Among the given options, only ascorbic acid qualifies as a vitamin.
I : 3 Q dentify the number of structure/s from the following which can be
corelated to D-glyceraldehyde.
JEE Main 2025 (Online) 22nd January Evening Shift
Options:
A. three
B. two
C. one
D. four
Ans: A
Solution:
In  group in right hand side then D-configuration is assigned. A , B , D - O H G : 4 Q iven below are two statements:
Statement I: Fructose does not contain an aldehydic group but still
reduces Tollen's reagent
Statement II: In the presence of base, fructose undergoes
rearrangement to give glucose.
In the light of the above statements, choose the correct answer from
the options given below
JEE Main 2025 (Online) 23rd January Morning Shift
Options:
A. Statement I is true but Statement II is false
B. Statement I is false but Statement II is true
C. Both Statement I and Statement II are true
D. Both Statement I and Statement II are false
Ans: C
Solution:
Page 5


T : 1 Q he total number of hydrogen bonds of a DNA-double Helix strand
whose one strand has the following sequence of bases is ________.
JEE Main 2025 (Online) 4th April Morning Shift
Ans: 33
Solution:
Two nucleic acid chains are wound about each other and held together by H bonds between pair of bases.
Adenine from two hydrogen bonds with thymine and Guanine form three hydrogen bond with cytosine.
In given DNA strand total seven guanine and cytosine bases which form total 21 H -bonds and six adenine
and thymine base which will form total 12 H -bonds with other DNA strand.
Total no. of H bonds 
Ans. 33
W : 3 2 Q hich of the following acids is a vitamin ?
JEE Main 2025 (Online) 22nd January Morning Shift
5 ' - G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' 5 ' G - G - C - A - A - A - T - C - G - G - C - T - A - 3 ' = 7 × 3 + 6 × 2 = 3 3 Options:
A. Saccharic acid
B. Ascorbic acid
C. Aspartic acid
D. Adipic acid
Ans: B
Solution:
Correct Answer: Ascorbic Acid
Ascorbic acid is commonly known as Vitamin C. It is a water-soluble vitamin essential for various bodily
functions, including:
Antioxidant Function: It helps protect cells from damage caused by free radicals.
Collagen Synthesis: It is crucial for the formation of collagen, a protein necessary for skin, cartilage,
tendons, ligaments, and blood vessels.
Immune Support: It supports the immune system by enhancing the body's natural defenses.
Iron Absorption: It improves the absorption of non-heme iron from plant-based foods.
Among the given options, only ascorbic acid qualifies as a vitamin.
I : 3 Q dentify the number of structure/s from the following which can be
corelated to D-glyceraldehyde.
JEE Main 2025 (Online) 22nd January Evening Shift
Options:
A. three
B. two
C. one
D. four
Ans: A
Solution:
In  group in right hand side then D-configuration is assigned. A , B , D - O H G : 4 Q iven below are two statements:
Statement I: Fructose does not contain an aldehydic group but still
reduces Tollen's reagent
Statement II: In the presence of base, fructose undergoes
rearrangement to give glucose.
In the light of the above statements, choose the correct answer from
the options given below
JEE Main 2025 (Online) 23rd January Morning Shift
Options:
A. Statement I is true but Statement II is false
B. Statement I is false but Statement II is true
C. Both Statement I and Statement II are true
D. Both Statement I and Statement II are false
Ans: C
Solution:
T : 5 Q he -Helix and  - Pleated sheet structures of protein are
associated with its :
JEE Main 2025 (Online) 23rd January Evening Shift
Options:
A. tertiary structure
B. quaternary structure
C. primary structure
D. secondary structure
Ans: D
Solution:
-helix and -pleated sheet belongs to secondary structure of protein, which have hydrogen bonds.
a ß a ß T : 6 Q he carbohydrate "Ribose" present in DNA, is
A. A pentose sugar
B. present in pyranose from
C. in "D" configuration
D. a reducing sugar, when free
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FAQs on JEE Main Previous Year Questions (2025): Biomolecules

1. What are the main types of biomolecules, and what roles do they play in living organisms?
Ans. The main types of biomolecules include carbohydrates, lipids, proteins, and nucleic acids. Carbohydrates serve as a primary energy source and structural components in cells. Lipids are essential for forming cell membranes, storing energy, and signaling. Proteins perform a variety of functions including catalyzing biochemical reactions (enzymes), transporting molecules, and providing structural support. Nucleic acids, such as DNA and RNA, store and transmit genetic information necessary for the growth and reproduction of organisms.
2. How do enzymes function as biological catalysts in biochemical reactions?
Ans. Enzymes are specialized proteins that accelerate biochemical reactions by lowering the activation energy required for the reaction to occur. They bind to specific substrates at their active sites, forming an enzyme-substrate complex. This interaction stabilizes the transition state, allowing the reaction to proceed more easily. After the reaction, the products are released, and the enzyme remains unchanged, ready to catalyze further reactions.
3. What is the significance of the structure of proteins in their biological function?
Ans. The structure of proteins is crucial to their function, which is determined by the sequence of amino acids and how they fold into specific three-dimensional shapes. Proteins have four levels of structure: primary (amino acid sequence), secondary (alpha helices and beta sheets), tertiary (overall 3D shape), and quaternary (multiple polypeptide chains). Any alteration in this structure can lead to a loss of function or diseases, highlighting the importance of the correct folding and stability of proteins.
4. What are the differences between DNA and RNA in terms of structure and function?
Ans. DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) have distinct structural and functional differences. DNA is double-stranded, contains the sugar deoxyribose, and uses thymine (T) as one of its bases. RNA, on the other hand, is usually single-stranded, contains the sugar ribose, and uses uracil (U) instead of thymine. Functionally, DNA stores and transmits genetic information, while RNA plays a role in protein synthesis and can have various functions, including acting as a messenger (mRNA), transfer (tRNA), or ribosomal component (rRNA).
5. How do carbohydrates contribute to energy metabolism in living organisms?
Ans. Carbohydrates are a primary source of energy for living organisms. They are broken down through processes such as glycolysis and the citric acid cycle to release energy stored in their chemical bonds. Simple carbohydrates, like glucose, are quickly utilized for immediate energy, while complex carbohydrates, like starch, are stored for later use. Additionally, carbohydrates play vital roles in cell signaling and recognition processes, contributing to overall metabolic functions.
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