Directions: Read the given passage carefully and answer the questions as follows:
Passage
New research by Paul Fildes and K. Whitaker challenges the theory that strains of bacteria can be “trained” to mutate by withholding a metabolite necessary for their regular function. In particular, they consider the case of bacteria typhosum, which needs tryptophan in order to reproduce. Earlier researchers had grown the bacteria in a medium somewhat deficient in tryptophan and observed the growth of mutant strains of the bacteria which did not need tryptophan in order to reproduce.
Fildes and Whitaker argue that the withholding of tryptophan did not induce these mutant strains of bacteria. Rather, these mutants were already present in the original sample of bacteria typhosum, albeit in a concentration too small to detect. In experimenting with the bacteria grown in agar cultures, they found that by plating out huge quantities of the bacteria, one could locate mutant strains. Because of the possibility that the lack of uniformity of the agar cultures had in fact trained mutant strains, they conducted similar experiments with liquid cultures and again found that mutant strains of the bacteria were present in the original sampling. From these experiments, Fildes and Whitaker conclude that the mutants are of genetic origin and are not induced by environmental training. They asserted that the concentration of tryptophan is unrelated to the appearance of these mutants in the bacteria.
To confirm these results, Fildes and Whitaker used an innovative plating technique using pile fabrics, such as velvet or velveteen, to accurately imprint the growth found on an original agar plate to a series of replica agar plates. The process entails taking the original agar plate, inverting it onto the velvet while using light finger pressure to transfer growth, and then imprinting the fabric, with its pattern of growth, on the new agar plates. By using this replica plating method, Fildes and Whitaker demonstrated that the mutants were in fact genetically present or preadapted, as the locations of the mutant strains of bacteria on the replica plates were identical to the locations of the mutant strains on the original agar plate.
Question for Practice Test: Reading Comprehension - 31
Try yourself:Which of the following most accurately states the purpose of the passage?
Explanation
The question asks for the purpose of the passage. The passage focuses on an experiment designed to challenge an existing theory about bacteria. The correct answer must take the entirety of the passage into account without misrepresenting its focus.
(A) The “innovative technique” is the replica plating method discussed in the third paragraph. This technique was used to support the scientific hypothesis of Fildes and Whitaker. Thus, the passage did not need to “defend [Fildes and Whitaker’s] scientific hypothesis from attack by [the] innovative technique.” In addition, the passage did not even defend the theory that Fildes and Whitaker were challenging.
(B) The first sentence of the passage makes it clear that the focus will be to “challenge the theory that strains of bacteria can be “trained” to mutate,” not to describe a process by which such training was accomplished.
(C) CORRECT: The second and third paragraphs discuss the experiments performed by Fildes and Whitaker that were designed to test the established theory, mentioned in the first paragraph, that “strains of bacteria can be “trained” to mutate.” This answer choice summarizes the entire passage.
(D) Fildes and Whitaker argue against a theory, not an established protocol. There is no mention in the passage that a particular protocol is outdated.
(E) The passage does not state that either Fildes and Whitaker’s theory, or the theory they were challenging was questionable. The passage describes, but does not “challenge,” several scientific techniques.
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Question for Practice Test: Reading Comprehension - 31
Try yourself:According to the passage, Fildes and Whitaker conducted the experiment with liquid culture because
Explanation
This is a specific detail question that asks what the passage states about the use of liquid culture. The use of liquid culture is discussed in the second paragraph.
(A) Both agar culture and tryptophan are mentioned in the passage, but are discussed separately: bacteria were grown in an agar culture, and certain bacteria require tryptophan in order to reproduce. The passage does not state that the agar culture contained tryptophan.
(B) Although plating is discussed in the second and third paragraphs, the difficulty of plating was not mentioned.
(C) The passage did not mention the number of bacteria that could be supported by the agar culture.
(D) CORRECT: According to the second paragraph of the passage, "because of the possibility that the lack of uniformity of the agar cultures had in fact trained mutant strains, they [Fildes and Whitaker] conducted similar experiments with liquid cultures..." Thus, the liquid culture is a more consistent, or uniform, medium.
(E) In the third paragraph, it is stated that “Fildes and Whitaker used an innovative plating technique using pile fabrics…” The plating process and the results demonstrated by this method are subsequently explained. The liquid culture experiments are discussed separately, in the second paragraph. The passage did not state that the agar culture did not adhere well to pile fabric.
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Question for Practice Test: Reading Comprehension - 31
Try yourself:It can be inferred from the passage that the replica plating method is effective for which of the following reasons?
Explanation
The replica plating method is discussed in the third paragraph. The first two sentences describe the process, while the third sentence explains what was learned by using this method. The correct inference about the effectiveness of the replica plating method will be a virtual rephrasing of the facts presented in the third paragraph.
(A) We cannot infer that the replica plating method allows researchers to determine the relative sizes of different populations of bacteria. The population sizes of various bacteria populations were never discussed.
(B) CORRECT: The last sentence of the passage states that “the locations of the mutant strains of bacteria on the replica plates were identical to the locations of the mutant strains on the original agar plate,” citing this as proof that “the mutants were in fact genetically present or preadapted.” In other words, the mutant strains did not develop after transfer from the original agar plate.
(C) The only type of bacteria mentioned in the passage was bacteria typhosum, so we cannot infer that the replica plating method eliminates the possibility that the agar culture was contaminated by a different type of bacteria.
(D) The role of tryptophan is discussed in the first and second paragraphs. This answer choice goes too far by asserting that “no tryptophan was present in the original agar culture.” We do not have enough information to make that inference.
(E) According to the first paragraph, “the necessary metabolites for bacterial reproduction” are defined as the substances “necessary for their (bacteria’s) regular function.” In the case of bacteria typhosum, that substance is tryptophan. We do not have enough information to infer that the replica plating method establishes that the original agar culture contained tryptophan. If fact, the presence of mutant strains of bacteria on the replica plates would contradict this statement rather than support it.
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