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NEET Exam  >  NEET Test  >  Biology Class 12  >  Test: Principles of Biotechnology (NCERT) - NEET MCQ

Principles of Biotechnology (NCERT) - Free MCQ Practice Test with solutions,


MCQ Practice Test & Solutions: Test: Principles of Biotechnology (NCERT) (10 Questions)

You can prepare effectively for NEET Biology Class 12 with this dedicated MCQ Practice Test (available with solutions) on the important topic of "Test: Principles of Biotechnology (NCERT)". These 10 questions have been designed by the experts with the latest curriculum of NEET 2026, to help you master the concept.

Test Highlights:

  • - Format: Multiple Choice Questions (MCQ)
  • - Duration: 10 minutes
  • - Number of Questions: 10

Sign up on EduRev for free to attempt this test and track your preparation progress.

Test: Principles of Biotechnology (NCERT) - Question 1
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Plasmid used to construct the first recombinant DNA was isolated from which bacterium species?

Detailed Solution: Question 1

The first recombinant DNA was constructed by Stanley Cohen and Herbert Boyer in 1972. They cut the piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of Escherichia coil.

NCERT Topic: Plasmid used to construct recombinant DNA

NCERT Line: "DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium."

Test: Principles of Biotechnology (NCERT) - Question 2
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The term 'molecular scissors' refers to

Detailed Solution: Question 2

The restriction endonuclease enzyme inspects the length of a DNA sequence. Once it recongnises specific sequence, it binds to the DNA and cuts each of the two strands of the double helix at specific points in their sugar phosphate back bone. Special sequence in the DNA recongnised by restriction endonuclease is called palindromic nucleotide sequence. Restriction endonuclease enzymes are also known as molecular scissors or biological scissors or chemical knives or chemical scalpels.

NCERT Topic: Restriction enzymes

NCERT Line: "Restriction enzymes belong to a larger class of enzymes called nucleases."

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Test: Principles of Biotechnology (NCERT) - Question 3
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The term 'recombinant DNA' refers to

Detailed Solution: Question 3

After cutting the source DNA and the vector DNA with a specific restriction enzyme, the cut out 'gene of interest' from the source DNA and the cut vector with space are mixed and ligase enzyme is added. This results in the formation of rDNA or hybrid DNA or chimeric DNA.

NCERT Topic: Recombinant DNA technology

NCERT Line: "Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice."

Test: Principles of Biotechnology (NCERT) - Question 4
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Which of the following statement is not correct?

Detailed Solution: Question 4

The statement that is not correct is:

3. Log phase does not show any significant increase in the number of cells whereas the lag phase shows rapid multiplication of cells.

Here's why:

  • Log phase (or exponential phase) is actually characterized by a rapid and significant increase in the number of cells. During this phase, cells are dividing at an exponential rate.

  • Lag phase is the initial phase where cells are adapting to new conditions and preparing for growth. During this phase, there is little to no significant increase in cell number as the cells are not yet dividing rapidly.

To summarize, the log phase is when cell multiplication is most rapid, not the lag phase.

Test: Principles of Biotechnology (NCERT) - Question 5
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Who is the father of genetic engineering?

Detailed Solution: Question 5

In 1972, genetic engineering was started by Paul Berg. He was able to introduce a gene of the SV-40 virus into a bacterium with the help of lambda phage. Berg is often considered as "Father of genetic engineering". He was awarded Nobel Prize in 1980.

Test: Principles of Biotechnology (NCERT) - Question 6
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The term 'chemical knife' refers to

Detailed Solution: Question 6

The restriction endonuclease enzyme inspects the length of a DNA sequence. Once it recognises specific sequence, it binds to the DNA and cuts each of the two strands of the double helix at specific points in their sugar phosphate back bone. Special sequence in the DNA recognised by restriction endonuclease is called palindromic nucleotide sequence. Restriction endonuclease enzymes are also known as molecular scissors or biological scissors or chemical knives or chemical scalpels.

NCERT Topic: Restriction enzymes

NCERT Line: "Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases."

Test: Principles of Biotechnology (NCERT) - Question 7
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One of the key factors, which makes the plasmid the vector in genetic engineering is

Detailed Solution: Question 7

Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeasts. Plasmids are usually not essential for normal cell growth and division, they often confer some traits to the host organism e.g., resistance to certain antibiotics. The plasmid that is used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA or cloning vector or gene carrier.

NCERT Topic: Plasmid as a vector

NCERT Line: "In the same way, a plasmid can be used as vector to deliver an alien piece of DNA into the host organism."

Test: Principles of Biotechnology (NCERT) - Question 8
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Genetic engineering is possible, because

Detailed Solution: Question 8

Genetic engineering is the artificail synthesis, isolation modification, cimbination, addition and repaor of the genetic material (DNA) to alter the phenotype of the host organism to suit human needs. It is the manipulation of genes by man in vitro. Restriction endonucleases play major role in gentic engineering as they can cut DNA at specific sites.

NCERT Topic: Cutting of DNA at specific locations

NCERT Line: "Restriction endonucleases are used in genetic engineering to form 'recombinant' molecules of DNA, which are composed of DNA from different sources/genomes."

Test: Principles of Biotechnology (NCERT) - Question 9
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Which of the following processes/techniques can be included under biotechnology?
(i) In vitro fertilisation
(ii) Synthesis of a gene
(iii) Correcting a defective gene
(iv) Developing a DNA vaccine

Detailed Solution: Question 9

Biotechnology deals with techniques of using live micro-organisms, plant or animal cells or their components or enzymes from organisms to produce products and processes (service) useful to human beings. In vitro fertilisation, synthesis of recombinant gene, correcting a defective gene and developing a DNA vaccine are all parts of biotechnology.

NCERT Topic: Processes included under biotechnology

NCERT Line: "Further, many other processes/techniques are also included under biotechnology. For example, in vitro fertilisation leading to a 'test-tube' baby, synthesising a gene and using it, developing a DNA vaccine or correcting a defective gene, are all part of biotechnology."

Test: Principles of Biotechnology (NCERT) - Question 10
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Match the scientists in column I with their related discoveries in column II and select the correct option from the given codes.

Detailed Solution: Question 10

  • The father of genetic engineering is Paul Berg. He creates the recombinant DNA technology in 1972.
  • Kary Mullis invents the polymerase chain reaction in 1993. Arber, Smith and Nathan discovered the restriction endonuclease enzymes in 1978.
  • Stanley Cohen and Herbert Boyer constructed artificial recombinant DNA for the first time. They got the idea of linking a gene encoding for antibiotic resistance with a native plasmid of Salmonella typhinurium.

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About this NEET Practice Test

This test contains 10 MCQs on Principles of Biotechnology (NCERT) with detailed solutions for each question. It is part of the Biology Class 12 course on EduRev, designed to align with the current NEET syllabus. Each solution explains not just the correct answer but also gives you an understanding of the topic — not just to attempt the question but also help you prepare for the exam with practice.

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