Biotechnology Engineering - BT 2012 GATE Paper (Practice Test) - GATE MCQ

Gender is not mentioned in the advertisement and (B) clearly eliminated

P and R always holds true Else consider a sample set {1, 2, 3, 4} and check accordingly

The mismatch repair deals with correcting mismatch of the normal bases using BER or NER enzyme systems. The system assumes that the parental strands are methylated and the freshly synthesized daughter strands are non methylated.

Both pUC and the bacterial own genome produce a faulty gene product of Lac Z gene. The lac Z fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic complementation with a defective form of 0-galactosidase enzyme encoded by host chromosome (mutation lacZDM15)

Immunoglobulin idiotypes are serologically defined determinants associated with the variable (V) region of antibody molecules

All the blood grouping reactions are agglutination reactions. In these the antibodies against A and B antigens when added, they bind to the cell surface and result in clumping of cells. These clumps or settling down of cells is referred to as agglutination.

All T cells originate from haematopoietic stem cells in the bone marrow. B cells undergo their complete maturation in the thymus while, T cells during their early stages of maturation migrate to the thymus and then undergo a process of maturation. They are released as competent and mature T cells from Thymus.

A humanized chain is a chain in which the complementarity determining regions (CDR) of the variable domains are foreign (originating from one species other than human, or synthetic) whereas the remaining chain is of human origin.

DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury.

A nude mouse is a laboratory mouse from a strain with a genetic mutation that causes a deteriorated or absent thymus, resulting in an inhibited immune system due to a greatly reduced number of T cells.

Heat-inactivation (heating to 56°C for 30 minutes) of serum is done to inactivate complement, a group of proteins present in sera that are part of the immune response.

In the G protein associated signal cascade system, binding of hormone to a cell surface receptor, like the 7TM (a 7 transmembrane alpha helices) which is a part of G protein. This will activate production of second messengers like cAMP by the enzyme adenylate cyclase. The cAMP in turn binds and activates down stream signal proteins line cAMP dependent protein kinase A (PKA).

To investigate the effect of serine 78 phosphorylation on p21 activity, replacement of serine 78 with aspartic acid is done, creating the phosphomimic p21^S78D.

Non sense mutations causes premature stop to be introduced that would lead to truncated or incomplete protein synthesis. If the protein synthesis has to be continued, another mutation in t-RNA gene would continue be advised to get full length polypeptide

The strength of ChIP assays is their ability to capture a snapshot of specific protein: DNA interactions occurring in a system and to quantitate the interactions using quantitative polymerase chain reaction (qPCR).

The direction of shell coiling in snail is a classical example for extra chromosomal inheritance, as in this it is determined by maternal gene effects and not that of the offspring.

During photo respiration under low CO2 and high O2 reacts with ribulose – 1, 5 bisphosphate to yield- one molecule each of 3-phosphoglycerate and 2- phosphoglycolate.

Colchicines is not a protoplast fusion inducing agent instead it functions as mitotic inhibitor

One katak is 6 x 10⁷ IU units

Enzymes accelerate the rate of the reverse reaction as well as the forward reaction, it would be helpful to ignore any back reaction by which E1P might form ES. The velocity of this back reaction would be given by v = k_-2[E][P].

Protein structural database: Primary database: PDB Secondary database: SCOP, CATH

A program for constructing phylogenetic tree is PHYLIP. It is a Phylogeny Inference Package. It is a free computational phylogenetic package which has programs for inferring evolutionary tree constructions.