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MCAT Exam  >  MCAT Test  >  Biochemistry  >  Test: Primary Structure of Protein - MCAT MCQ

Primary Structure of Protein - Free MCQ Practice Test with solutions, MCAT


MCQ Practice Test & Solutions: Test: Primary Structure of Protein (10 Questions)

You can prepare effectively for MCAT Biochemistry for MCAT with this dedicated MCQ Practice Test (available with solutions) on the important topic of "Test: Primary Structure of Protein". These 10 questions have been designed by the experts with the latest curriculum of MCAT 2026, to help you master the concept.

Test Highlights:

  • - Format: Multiple Choice Questions (MCQ)
  • - Duration: 10 minutes
  • - Number of Questions: 10

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Test: Primary Structure of Protein - Question 1
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The structure formed by joining the amino acids by a peptide bond is called ________ structure of a protein.

Detailed Solution: Question 1

When amino acids are just linked together by a peptide bond to form a long linear chain of a polypeptide, it is called the primary structure of proteins. Secondary, tertiary, and Quaternary structure are all higher levels of organization and require non-covalent interactions.

Test: Primary Structure of Protein - Question 2
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Which of the following represents the two-dimensional structure of proteins?

Detailed Solution: Question 2

The primary structure of Protein represents the two-dimensional structure of proteins. The primary structure of proteins just contains amino acids linked together to form a long chain of a polypeptide. Quaternary, tertiary, and secondary structure refers to the three-dimensional structure of proteins.

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Test: Primary Structure of Protein - Question 3
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The polyacrylamide gel electrophoresis (PAGE) of proteins is based on the following properties of proteins.

Detailed Solution: Question 3

The separation in PAGE is based on gel filtration i.e. the size and shape, as well as electrophoretic mobility i.e. electric charge. There are two types of PAGE viz. i) SDS PAGE and ii) Native PAGE.

Test: Primary Structure of Protein - Question 4
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Trypsin cleaves peptide bonds on the ‘c’ terminal side of the positively charged residues Arg and Lys, if the next residues is not _____

Detailed Solution: Question 4

Trypsin is a proteolytic enzyme with high specificity. It cleaves the peptide bond on the ‘c’ terminal side of the positively charged residues Arg and Lys, if the next residues is not Pro. It is used in the cleavage of protein molecules for protein sequencing.

Test: Primary Structure of Protein - Question 5
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________ is used for sequencing of peptides.

Detailed Solution: Question 5

Mass spectrometry is a technique that measures the mass to charge (m/z) ratio for the ions in the gas phase. Therefore, mass spectrometry is used for the sequencing of peptides. Mass spectrometry can also be used to identify a protein; hence, it is also called protein mass fingerprinting.

Test: Primary Structure of Protein - Question 6
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A polypeptide chain contains two terminals – one carboxyl-terminal (C terminal) and the other amino-terminal (N terminal). Which of the following is true?

Detailed Solution: Question 6

During translation, the first amino acid attached has an amino free end and the last amino acid has a carboxyl free end. Hence, the N terminal is represented on the left side and C terminal on the right side.

Test: Primary Structure of Protein - Question 7
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Which of the following interactions is crucial for the primary structure of proteins?

Detailed Solution: Question 7

Protein’s primary structure is made of amino acids linked together by a peptide bond. Thus, the peptide bond is crucial for the primary structure of proteins. Hydrogen bond, disulfide bond, Vander Waals interactions are all required in a higher level of organization.

Test: Primary Structure of Protein - Question 8
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For a protein with 100 amino acids, how many possible sequences are there?

Detailed Solution: Question 8

The general rule is, for a protein having ‘n’ residues, there are (20)n possible combinations. There are 20 different types of amino acids occurring naturally in proteins. Therefore, (20)100 is the correct answer.

Test: Primary Structure of Protein - Question 9
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In metal chelate affinity chromatography, divalent cations such as Zn+2 or Ni+2 is attached on the electrophoretic matrix and it binds to ___________

Detailed Solution: Question 9

When 6 histidine residues are inserted consecutively through recombinant DNA technology, it is called as His tag. It has a high affinity for nickel. This strategy is used for the purification of recombinant proteins.

Test: Primary Structure of Protein - Question 10
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______ is used as a reducing agent in SDS PAGE.

Detailed Solution: Question 10

2-mercaptoethanol (beta-mercaptoethanol) is used as a reducing agent in SDS PAGE. It breaks the disulfide bonds and denatures the protein molecule. SDS is not a reducing agent, it disrupts non-covalent interactions between peptides. Ammonium persulphate (APS) and Bisacrylamide do not show any reducing properties.

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About this MCAT Practice Test

This test contains 10 MCQs on Primary Structure of Protein with detailed solutions for each question. It is part of the Biochemistry for MCAT course on EduRev, designed to align with the current MCAT syllabus. Each solution explains not just the correct answer but also gives you an understanding of the topic — not just to attempt the question but also help you prepare for the exam with practice.

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