The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequence of steps?
Given table gives an account of differences between PCR and gene cloning.. Which of the following points show the incorrect differences?
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Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium ________.
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in a polymerase chain reaction (PCR)?
After completion of the biosynthetic stage in the bioreactors, the product undergoes separation and purification processes, collectively termed as __________.
Eukaryotic genes do not function properly when cloned into a bacterial cell because
Match column I with column II and select the correct answer from the given codes.
Which of the following are the types of bioreactors?
(i) Simple stirred-tank bioreactor
(ii) Complex Stirred-tank bioreactor
(iii) Sparged stirred-tank bioreactor
(iv) Agitator stirred-tank bioreactor
Read statements (i)-(iv). Which of the following statements are incorrect?
(i) First transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
(ii) Restriction enzymes are used in isolation of DNA from other macromolecules.
(iii) Downstream processing is one of the steps of rDNA technology.
(iv) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.
An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can __________.