Read the given statements and select the correct option.
Statement 1: The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.
Statement 2: Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.
The restriction enzyme responsible for the cleavage of following sequence is
5' - G - T - C - G - A - c - 3'
3' - C - A - G - C - T - G - 5'
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Read the following statements and select the correct ones.
(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge
(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast
(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule
(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13
Read the given statements and select the correct option.
Statement 1: The tumour inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
Statements 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.
If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______.
If you want to recover many copies of the target DNA, you will choose a vector
The term “competent” refers to
In the process of insertional inactivation
Which of the following is required for microinjection method of gene transfer?
In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of