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DNA Replication: Helicase Video Lecture | Biology Class 12 - NEET

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FAQs on DNA Replication: Helicase Video Lecture - Biology Class 12 - NEET

1. What is the role of helicase in DNA replication?
Ans. Helicase is an enzyme that plays a crucial role in DNA replication. Its main function is to unwind and separate the double-stranded DNA into two single strands during the replication process. By breaking the hydrogen bonds between the base pairs, helicase creates a replication fork, allowing other enzymes and proteins to access the DNA strands and initiate replication.
2. How does helicase work during DNA replication?
Ans. Helicase binds to the origin of replication, which is a specific sequence on the DNA strand. Once bound, helicase uses ATP energy to move along the DNA molecule, separating the two strands by breaking the hydrogen bonds between the base pairs. This unwinding action creates a replication fork, where DNA polymerase and other enzymes can begin synthesizing new DNA strands.
3. Can helicase unwind the entire DNA molecule during replication?
Ans. No, helicase cannot unwind the entire DNA molecule during replication. The DNA molecule is extremely long, consisting of millions or billions of base pairs. Helicase can only unwind a small section of the DNA at a time, typically around 100-200 base pairs. As helicase moves along the DNA, it continuously unwinds and separates the strands, allowing for localized replication to occur.
4. Are there any other functions of helicase besides DNA replication?
Ans. Yes, helicase is involved in various cellular processes besides DNA replication. It plays a role in DNA repair, where it unwinds DNA strands to facilitate the removal of damaged or mismatched nucleotides. Helicase is also involved in transcription, the process of synthesizing RNA from a DNA template. Additionally, helicase is important in DNA recombination, where it helps exchange genetic material between DNA molecules.
5. Are there different types of helicase enzymes?
Ans. Yes, there are several types of helicase enzymes that are specialized for different cellular processes. In DNA replication, the main helicase enzyme is called the replicative helicase. Other types of helicases are involved in DNA repair, recombination, and transcription. Each type of helicase has specific functions and may act on different DNA sequences or structures.
158 videos|393 docs|239 tests
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