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Which organelle is wrongly paired?
Insertional inactivation helps in:
Having two antibiotic resistant genes in the same plasmid:
DNA can be isolated from fungi using chitinase as _______.
The component which is not required in PCR is _______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
In agarose gel electrophoresis, the DNA fragments separate out (resolve) according to their size or length because of the sieving property of agarose gel. It means, the smaller the fragment size, the farther it will move.
Which one is called molecular scissors?
Restriction enzymes are also called 'molecular scissors' as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.
Denaturation is done at high temperature as it leads to:
Steps involved in genetic engineering are:
1. Maintenance of introduced DNA in the host.
2. Transfer of DNA in its progeny.
3. Identifying DNA with desirable genes.
4. Introduction of identified DNA into the host.
Genetic engineering involves:
(i) Identifying DNA with desirable genes.
(ii) Introduction of identified DNA into the host.
(iii) Maintenance of introduced DNA in the host.
(iv) Transfer of DNA in its progeny.
Hence, option C is correct.
Origin of replication is the specific DNA sequence on chromosome that which is responsible for:
The group of letters that form same words when read both forward and backward are called?
The groups of letters that form same words when read both forward and backward are called Palindrome. For example MALYALAM which read same from both side.
Enzymes used to join foreign DNA to plasmid are _________.
The Enzymes used to join foreign DNA to plasmid is ligases. The plasmid of bacteria replicate this DNA strands along with other which can be further transferred to target cells.
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
The separated DNA fragments can he visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to ultraviolet radiation (we cannot see pure DNA fragments in the visible light and without staining).
‘R’ in EcoRI restriction endonuclease denotes:
EcoRI comes from Escherichia coli RY 13. In EcoRI, the letter ‘R’ is derived from the name of strain.
Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.
Reason: We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.
The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. We can see bright orange coloured bands of DNA in aethidium bromide stained gel exposed to UV light.
During agarose gel, electrophoresis DNA fragments move towards the anode. This is because _________.
Rop genes in pBR322 codes for _________.
The particles used to coat with DNA in Biolistic gun is of:
DNA is coated with tungsten before used in biolistic gun for inserting the DNA directly into target cells for obtaining particular protein.
Production of large scale recombinant products can be done in _________.
Bioreactor is used to produce large scale production. Bioreactor is a large vessels in which large scale raw materials are biologically converted into specific products.
Sparged stirred tank bioreactor is advantageous over the simply stirred tank bioreactor as _________.
The X-gal will be converted into a coloured product when:
The X-gel will be converted into a coloured product when B-galactosidasse acts on it.
Blades in a bioreactor help in?
The restriction sites of the nucleoid are methylated because:
The restriction sites of the nucleoid are methylated because it protects bacteria from its own enzymes.
A ladder is used in Gel electrophoresis as it helps in _________.