Test: Transcription (NCERT) - NEET MCQ

mRNA has methylated region at the 5' terminus. It functions as a cap for attachment with ribosome. Cap is followed by an initiation codon (AUG) either immediately or after a small non-coding leader region. Then there is coding region followed by termination codons (UAA, UAG OF UGA). After termination codon there is a small non-coding trailer region and poly A area at the 3' terminus. These leader and trailer regions are called UTRs (Untranslated regions). Cap at 5' end and poly-A tail at 3' end also serves to protect mRNA from enzymatic decay.

The RNA polymerase enzyme binds to a specific site called promoter and initiates transcription. The promoter region determines which DNA strand is to be transcribed. Thus, a promoter region has RNA polymerase recognition site and RNA polymerase binding site .

mRNA strand is complementary to one of the DNA strands i.e., template strand. In RNA, uracil is present instead of thymine which is complementary to adenine. Cytosine and guanine are also complementary to each other. Hence, the sequence of bases in transcript would be CGAAUCCGUU.

At 5' end of hnRNA, a cap is formed by modification of GTP into 7-methyl guanosine or 7_m(G_ppp). This process is called capping and it protects the mRNA from degradation by nucleases and also.

Eukaryotes have three RNA polymerases. RNA polymerase I is located in the nucleolus and transcribes for RNAs (28S, 18S and 5.8S) whereas RNA polymerase II is localised in the nucleoplasm and used for hnRNA, mRNA and RNA polymerase Ill is localised in the nucleus, possibly the nucleolar-nucleoplasm interface and transcribes for tRNA, 5S, RNA and nRNAs.

In eukaryotes, the coding sequences or expressed sequences are defined as exons. Exons appear in mature or processed RNA. The exons are interrupted by introns, they do not appear in mature or processed RNA.

Monocistronic genes typically have interrupted coding sequences (exons), whereas polycistronic genes often have continuous coding sequences.

Polycistronic structural genes are mostly found in prokaryotes, such as bacteria, where multiple genes are often transcribed together into a single mRNA molecule.

As transcription proceeds the initiation and elongation steps are mediated by the RNA Polymerase enzyme (DNA dependent RNA polymerase). As a result, the RNA transcript is synthesized as a single-stranded structure and is released when the Rho factor binds to the enzyme RNA polymerase to terminate the transcription. 

The primary mRNA transcript is longer and localised into the nucleus, where it is also called heterogeneous nuclear RNA (hnRNA) or pre-mRNA. At the 5' end of hnRNA, a cap (consisting of 7-emthyl guanosine triphosphate or 7 mG) and a tail of poly A (Adenylate residues) at the 3' end are added. These processes are respectively called as capping and tailing. The cap is a chemically modified molecule of guanosine triphosphate (GTP). The primary mRNA are made up of two types of segments, non-coding introns and the coding exons. The introns are removed by a process called RNA splicing and the exons are joined in a defined order.

The process of copying genetic information from one strand of the DNA into RNA is termed as transcription. Unlike the process of replication, which once sets in, the total DNA of an organism gets duplicated, in transcription only a segment of DNA and only one of the strands is copied into RNA.

The given diagram represents post-transcriptional processing resulting in the formation of mRNA. Since, introns and exons are present, it is transcription in eukaryotes.

Exons are the coding sequences in eukaryotic genes that contain the genetic information necessary for protein synthesis.

Transcription unit is the distance between sites of initiation and termination by RNA polymerase. It may include more than one gene. RNA polymerase produces transcription unit that extends from the promoter to the termination sequences.

Heterogeneous nuclear RNA or hnRNA is a term that encompasses various types and sizes of RNAs found in the eukaryotic cell nucleus. When this undergoes modifications and processing it will give rise to the mature mRNA that has the inherent code for the protein synthesis (amino acid code). The mRNA in eukaryotes is synthesized in the nucleus and hence should be transported to the cytoplasm and into the ribosome for protein synthesis by the process of translation.