|
 Biotechnology: Principle & Processes 30 Flashcards |
Start
|
|
What are the two core techniques of modern biotechnology? |
Card: 1 / 30 |
|
|
Genetic engineering and bioprocess engineering. |
Card: 2 / 30 |
|
|
Cuboidal epithelium is commonly found in ___ and ___. |
Card: 3 / 30 |
|
|
Kidney tubules and glands |
Card: 4 / 30 |
|
|
True or False: Restriction enzymes can only cut DNA at random locations. |
Card: 5 / 30 |
|
|
False. Restriction enzymes cut DNA at specific palindromic recognition sequences. |
Card: 6 / 30 |
|
|
What is insertional inactivation in cloning vectors? |
Card: 7 / 30 |
|
|
Insertion of foreign DNA inactivates a gene, allowing recombinant colonies to be identified by lack of color.
|
Card: 8 / 30 |
|
|
What is a restriction endonuclease? |
Card: 9 / 30 |
|
|
An enzyme that cuts DNA at specific palindromic recognition sequences, producing sticky ends.
|
Card: 10 / 30 |
|
|
Riddle: I am a process that amplifies DNA billion-fold, using cycles of heating and cooling. What am I? |
Card: 11 / 30 |
|
|
Polymerase Chain Reaction (PCR).
|
Card: 12 / 30 |
|
|
Fill in the blank: The first recombinant DNA was created in ___ by linking an antibiotic resistance gene to a plasmid. |
Card: 13 / 30 |
|
|
1972 |
Card: 14 / 30 |
|
|
What is the main purpose of gel electrophoresis in biotechnology? |
Card: 15 / 30 |
|
|
 Unlock all Flashcards with EduRev Infinity Plan Starting from @ ₹99 only
|
|
|
|
It separates DNA fragments by size, allowing for analysis of DNA samples. |
Card: 16 / 30 |
|
|
What are bioreactors used for in biotechnology? |
Card: 17 / 30 |
|
|
They are vessels for large-scale culture of cells, providing optimal conditions for product production.
|
Card: 18 / 30 |
|
|
What is the function of DNA ligase in recombinant DNA technology? |
Card: 19 / 30 |
|
|
It joins DNA fragments by forming bonds between their ends, creating recombinant DNA. |
Card: 20 / 30 |
|
|
Fill in the blank: Agrobacterium tumefaciens is used to transfer genes into ___ cells. |
Card: 21 / 30 |
|
|
Plant |
Card: 22 / 30 |
|
|
What are the steps of recombinant DNA technology? |
Card: 23 / 30 |
|
|
Isolation of DNA, cutting DNA, amplification (PCR), ligation, transformation, culturing, and extraction of the product.
|
Card: 24 / 30 |
|
|
Riddle: I am a small DNA ringlet that replicates independently in bacterial cells. What am I? |
Card: 25 / 30 |
|
|
A plasmid.
|
Card: 26 / 30 |
|
|
What is the significance of palindromic sequences in DNA? |
Card: 27 / 30 |
|
|
They are recognized by restriction enzymes and allow for the cutting and ligation of DNA strands. |
Card: 28 / 30 |
|
|
Fill in the blank: Competent host cells can take up recombinant DNA through methods like heat shock and ___. |
Card: 29 / 30 |
|
|
Micro-injection
|
Card: 30 / 30 |
|
|
Completed! Keep practicing to master all of them. |
Restart
|
