What is the function of restriction endonucleases in recombinant DNA technology?

Restriction endonucleases cleave DNA at specific sequences, allowing for the manipulation and recombination of DNA fragments.

Fill in the blank: A restriction map shows the specific locations where a particular ______ cuts genomic DNA.

Restriction endonuclease

True or False: Cosmids can carry DNA fragments ranging from 10 to 20 kilobases.

False. Cosmids can carry larger DNA fragments, typically ranging from 30 to 50 kilobases.

What is chimeric DNA?

Chimeric DNA is formed by combining DNA to be cloned with vector DNA.

Riddle: I can be sticky or blunt, cut DNA with precision, and help create new genes in a laboratory's mission. What am I?

Restriction endonuclease

Which enzyme is used to ligate DNA fragments during the formation of chimeric DNA?

DNA ligase

Fill in the blank: The term '__________' refers to a method used to overcome problems associated with sticky or blunt ends in DNA cloning.

Homopolymer Tailing

True or False: Blue-white screening is used to identify successful ligation of DNA by observing the color of bacterial colonies.

True.

What is the main function of CRISPR-Cas9 in gene editing?

CRISPR-Cas9 is used for precise gene editing by targeting and cutting specific DNA sequences.

Which type of DNA library contains the entire genomic DNA of an organism?

Genomic DNA Library

Fill in the blank: cDNA is synthesized from ______ using reverse transcriptase.

MRNA

True or False: The polymerase chain reaction (PCR) is primarily used for detecting RNA sequences.

False. PCR is used to amplify specific DNA sequences.

What is the role of Taq polymerase in PCR?

Taq polymerase synthesizes new DNA strands during the extension phase of PCR.

Riddle: I amplify DNA exponentially, allowing scientists to see, what is this technique that makes millions from a single copy?

Polymerase Chain Reaction (PCR)

In Southern blotting, what is used to transfer denatured DNA fragments to a membrane?

Blotting

Fill in the blank: Northern blotting is primarily used for detecting specific ______ molecules.

RNA

True or False: Western blotting is a technique used to detect specific DNA sequences.

False. Western blotting is used to detect specific proteins.

What is the purpose of using G-banding in cytogenetic analysis?

G-banding is used to visualize and analyze chromosomes based on their distinctive banding patterns.

Fill in the blank: The technique used to create monoclonal antibodies by fusing spleen cells with myeloma cells is called ______.

Hybridoma technique

What does RFLP stand for and what does it analyze?

RFLP stands for Restriction Fragment Length Polymorphism, and it analyzes genetic differences based on how restriction endonucleases cut DNA.

Riddle: I can reveal genetic variations, even if I’m just a fragment; changes in my patterns help you see what’s significant. What am I?

Restriction Fragment Length Polymorphism (RFLP)

Fill in the blank: Gene therapy involves the introduction of therapeutic genes into ______ cells.

Somatic

True or False: The Human Genome Project aimed to map the entire RNA sequence of the human genome.

False. The Human Genome Project aimed to map the entire DNA sequence of the human genome.

What is the primary purpose of bioinformatics in genetics?

Bioinformatics deals with the collection, storage, and analysis of biological data, particularly focusing on DNA and protein sequences.

Fill in the blank: The ______ Project identifies Single Nucleotide Polymorphisms (SNPs) associated with common human diseases.

HapMap

What are SNP arrays used for in genetic research?

SNP arrays are used to identify single nucleotide polymorphisms throughout the genome.

Which of the following vectors can carry DNA fragments ranging from 30 to 50 kilobases? A) Plasmids B) Cosmids C) BACs D) YACs

B) Cosmids

Fill-in-the-blanks: The technique used to create chimeric DNA involves treating both the foreign DNA and vector DNA with the same ______.

Restriction endonuclease

True or False: Sticky ends produced by restriction endonucleases are always more efficient for ligation than blunt ends.

False. While sticky ends are often more efficient for ligation, they can also lead to unwanted self-ligation or heterogeneous inserts.

What is the primary role of the CRISPR-Cas9 system in genetics?

It is used for precise gene editing and regulation by targeting and modifying specific DNA sequences.