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 Tools of Recombinant DNA Technology 20 Flashcards |
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Restriction enzymes are also known as ___ and are crucial for cutting DNA at specific sequences. |
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Restriction endonucleases |
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True or False: Restriction enzymes can cut DNA at any random location. |
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False. They cut DNA at specific recognition sequences. |
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What is the primary role of ligases in recombinant DNA technology? |
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Ligases join DNA fragments together after they have been cut by restriction enzymes. |
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Plasmids can replicate independently of ___ in bacterial cells. |
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Chromosomal DNA |
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Riddle: I help in cloning and have a circular shape, I can carry foreign DNA with ease. What am I? |
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A plasmid |
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What is the significance of sticky ends in recombinant DNA formation? |
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Sticky ends allow DNA fragments cut by the same restriction enzyme to bind together through complementary base pairing. |
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Fill in the blank: Vectors for plant gene cloning often utilize the ___ plasmid from Agrobacterium tumefaciens. |
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Ti |
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True or False: Gel electrophoresis is used to visualize DNA fragments after they have been separated. |
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True |
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What method uses heat shock to facilitate the uptake of recombinant DNA into bacterial cells? |
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Heat Shock Method |
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How do disarmed pathogen vectors introduce recombinant DNA into host cells? |
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They infect the cells and deliver recombinant DNA as part of their infection process. |
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