Page 1
IIT JAM BIOTECHNOLOGY 2022
QUESTION
Section A: Q.1 – Q.10 Carry ONE mark each.
1. Which of the following is involved in innate
immune response in higher mammals?
(A) T cell antigen receptor
(B) B cell antigen receptor
(C) Toll-like receptor
(D) Major histocompatibility complex-II molecule
2. Which among the following belongs to the family
“Retroviridae”?
(A) Human Immunodeficiency virus
(B) Ebola virus
(C) Dengue virus
(D) Influenza virus
3. Which of the following is a glycolipid?
(A) Cerebroside
(B) Phosphatidylcholine
(C) Phosphatidylserine
(D) Cardiolipin
4. Which of the following bacterial component
contains “dipicolinic acid”?
(A) Endospore
(B) Capsule
(C) Flagella
(D) Pili
5. The fossilization process in which mineral rich
water penetrates through the pores of
decomposed organic matter is known as _____.
(A) Carbonization
(B) Chemical fossilization
(C) Petrifaction
(D) Microfossilization
6. A random fluctuation in gene frequency is called
(A) Genetic drift
(B) Genetic load
(C) Panmixis
(D) Genetic shift
7. The number of “Barr Bodies” present in a somatic
cell of a woman suffering from Turner syndrome
is ____.
(A) 0
(B) 1
(C) 2
(D) 3
8. Which of the following are produced by Mangrove
trees to survive in the waterlogged swampy
forests?
(A) Trichomes
(B) Pneumatophores
(C) Spermatophores
(D) Cambia
9. Indeterminate growth in plants is due to the
presence of perpetually undifferentiated tissues,
called as ______.
(A) Tracheids
(B) Meristems
(C) Parenchyma
(D) Sclerenchyma
10. The osmotic potential (?) of pure water is _____
MPa.
(A) -1
(B) 0
(C) 0.1
(D) 10
Section A: Q.11 – Q.30 Carry TWO marks each.
11. Bacteria containing a tuft of flagella that comes
out from one pole is called ___.
(A) Lophotrichous
(B) Peritrichous
(C) Monotrichous
(D) Amphitrichous
12. Which of the following activity is associated with
Klenow fragment?
(A) 5'-3' exonuclease activity
(B) 5'-3' endonuclease activity
(C) Polymerase activity
(D) 3'-5' endonuclease activity
13. A frameshift mutation is caused by ____.
(A) 5-Bromouracil
(B) Acridine
(C) Glutathione
(D) Hypoxanthine
14. The zone of a pond system where respiration is
more than production is called as _____.
(A) Limnetic zone (B) Littoral zone
(C) Epilimnion zone (D) Benthic zone
Page 2
IIT JAM BIOTECHNOLOGY 2022
QUESTION
Section A: Q.1 – Q.10 Carry ONE mark each.
1. Which of the following is involved in innate
immune response in higher mammals?
(A) T cell antigen receptor
(B) B cell antigen receptor
(C) Toll-like receptor
(D) Major histocompatibility complex-II molecule
2. Which among the following belongs to the family
“Retroviridae”?
(A) Human Immunodeficiency virus
(B) Ebola virus
(C) Dengue virus
(D) Influenza virus
3. Which of the following is a glycolipid?
(A) Cerebroside
(B) Phosphatidylcholine
(C) Phosphatidylserine
(D) Cardiolipin
4. Which of the following bacterial component
contains “dipicolinic acid”?
(A) Endospore
(B) Capsule
(C) Flagella
(D) Pili
5. The fossilization process in which mineral rich
water penetrates through the pores of
decomposed organic matter is known as _____.
(A) Carbonization
(B) Chemical fossilization
(C) Petrifaction
(D) Microfossilization
6. A random fluctuation in gene frequency is called
(A) Genetic drift
(B) Genetic load
(C) Panmixis
(D) Genetic shift
7. The number of “Barr Bodies” present in a somatic
cell of a woman suffering from Turner syndrome
is ____.
(A) 0
(B) 1
(C) 2
(D) 3
8. Which of the following are produced by Mangrove
trees to survive in the waterlogged swampy
forests?
(A) Trichomes
(B) Pneumatophores
(C) Spermatophores
(D) Cambia
9. Indeterminate growth in plants is due to the
presence of perpetually undifferentiated tissues,
called as ______.
(A) Tracheids
(B) Meristems
(C) Parenchyma
(D) Sclerenchyma
10. The osmotic potential (?) of pure water is _____
MPa.
(A) -1
(B) 0
(C) 0.1
(D) 10
Section A: Q.11 – Q.30 Carry TWO marks each.
11. Bacteria containing a tuft of flagella that comes
out from one pole is called ___.
(A) Lophotrichous
(B) Peritrichous
(C) Monotrichous
(D) Amphitrichous
12. Which of the following activity is associated with
Klenow fragment?
(A) 5'-3' exonuclease activity
(B) 5'-3' endonuclease activity
(C) Polymerase activity
(D) 3'-5' endonuclease activity
13. A frameshift mutation is caused by ____.
(A) 5-Bromouracil
(B) Acridine
(C) Glutathione
(D) Hypoxanthine
14. The zone of a pond system where respiration is
more than production is called as _____.
(A) Limnetic zone (B) Littoral zone
(C) Epilimnion zone (D) Benthic zone
IIT JAM BIOTECHNOLOGY 2022
15. An organism that causes obstruction of lymphatic
system in humans is ____.
(A) Borrelia burgdorferi
(B) Brucella abortus
(C) Yersinia pestis
(D) Wuchereria bancrofti
16. A man having a dominant genetic trait (TT
genotype) can taste phenylthiocarbamide (PTC),
marries a woman who cannot taste PTC. The PTC
tasting ability of their biological son and daughter
is
(A) Son taster; Daughter non-taster
(B) Daughter taster; Son non-taster
(C) Both are non-tasters
(D) Both are tasters
17. Which of the following enzymes is absent in a
person suffering from Alkaptonuria?
(A) Tyrosinase
(B) Homogentisic acid oxidase
(C) Catechol dioxygenase
(D) Phenylalanine hydroxylase
18. The bacterium that can tolerate high
concentrations of salt and also ferment mannitol
is
(A) Staphylococcus aureus
(B) Staphylococcus epidermis
(C) Streptococcus pyogenes
(D) Serratia marcescens
19. Match the following
Group I Group II
P) Streptomycin 1) Inhibits beta-subunit of
RNA polymerase
Q) Cycloheximide 2) Inhibits peptidyl
transferase activity of
50S subunit
R) Rifamycin 3) Inhibits peptidyl
transferase activity of
60S subunit
S) Chloramphenicol 4) Inhibits binding of formyl
methionine tRNA to
ribosome
(A) P-1, Q-3, R-4, S-2
(B) P-4, Q-3, R-1, S-2
(C) P-2, Q-3, R-1, S-4
(D) P-3, Q-4, R-1, S-2
20. The major product formed in the given reaction
ANS. B
21. DNA gyrase can
(A) cut single-stranded DNA
(B) relax supercoiled DNA
(C) introduce negative supercoiling in DNA
(D) not utilize ATP
22. The stationary phase of cation-exchange
chromatography can be
(A) DEAE-cellulose
(B) CM-cellulose
(C) Sephadex G-50
(D) Heparin-Sepharose
23. Components of a Transmission Electron
Microscope are
(A) Electron gun, objective lens, positron beam,
projector lens
(B) Neutron beam, projector lens, objective lens,
evacuated tube
(C) Electron beam, projector lens, objective lens,
condenser lens
(D) X-ray beam, projector lens, objective lens,
condenser lens
Page 3
IIT JAM BIOTECHNOLOGY 2022
QUESTION
Section A: Q.1 – Q.10 Carry ONE mark each.
1. Which of the following is involved in innate
immune response in higher mammals?
(A) T cell antigen receptor
(B) B cell antigen receptor
(C) Toll-like receptor
(D) Major histocompatibility complex-II molecule
2. Which among the following belongs to the family
“Retroviridae”?
(A) Human Immunodeficiency virus
(B) Ebola virus
(C) Dengue virus
(D) Influenza virus
3. Which of the following is a glycolipid?
(A) Cerebroside
(B) Phosphatidylcholine
(C) Phosphatidylserine
(D) Cardiolipin
4. Which of the following bacterial component
contains “dipicolinic acid”?
(A) Endospore
(B) Capsule
(C) Flagella
(D) Pili
5. The fossilization process in which mineral rich
water penetrates through the pores of
decomposed organic matter is known as _____.
(A) Carbonization
(B) Chemical fossilization
(C) Petrifaction
(D) Microfossilization
6. A random fluctuation in gene frequency is called
(A) Genetic drift
(B) Genetic load
(C) Panmixis
(D) Genetic shift
7. The number of “Barr Bodies” present in a somatic
cell of a woman suffering from Turner syndrome
is ____.
(A) 0
(B) 1
(C) 2
(D) 3
8. Which of the following are produced by Mangrove
trees to survive in the waterlogged swampy
forests?
(A) Trichomes
(B) Pneumatophores
(C) Spermatophores
(D) Cambia
9. Indeterminate growth in plants is due to the
presence of perpetually undifferentiated tissues,
called as ______.
(A) Tracheids
(B) Meristems
(C) Parenchyma
(D) Sclerenchyma
10. The osmotic potential (?) of pure water is _____
MPa.
(A) -1
(B) 0
(C) 0.1
(D) 10
Section A: Q.11 – Q.30 Carry TWO marks each.
11. Bacteria containing a tuft of flagella that comes
out from one pole is called ___.
(A) Lophotrichous
(B) Peritrichous
(C) Monotrichous
(D) Amphitrichous
12. Which of the following activity is associated with
Klenow fragment?
(A) 5'-3' exonuclease activity
(B) 5'-3' endonuclease activity
(C) Polymerase activity
(D) 3'-5' endonuclease activity
13. A frameshift mutation is caused by ____.
(A) 5-Bromouracil
(B) Acridine
(C) Glutathione
(D) Hypoxanthine
14. The zone of a pond system where respiration is
more than production is called as _____.
(A) Limnetic zone (B) Littoral zone
(C) Epilimnion zone (D) Benthic zone
IIT JAM BIOTECHNOLOGY 2022
15. An organism that causes obstruction of lymphatic
system in humans is ____.
(A) Borrelia burgdorferi
(B) Brucella abortus
(C) Yersinia pestis
(D) Wuchereria bancrofti
16. A man having a dominant genetic trait (TT
genotype) can taste phenylthiocarbamide (PTC),
marries a woman who cannot taste PTC. The PTC
tasting ability of their biological son and daughter
is
(A) Son taster; Daughter non-taster
(B) Daughter taster; Son non-taster
(C) Both are non-tasters
(D) Both are tasters
17. Which of the following enzymes is absent in a
person suffering from Alkaptonuria?
(A) Tyrosinase
(B) Homogentisic acid oxidase
(C) Catechol dioxygenase
(D) Phenylalanine hydroxylase
18. The bacterium that can tolerate high
concentrations of salt and also ferment mannitol
is
(A) Staphylococcus aureus
(B) Staphylococcus epidermis
(C) Streptococcus pyogenes
(D) Serratia marcescens
19. Match the following
Group I Group II
P) Streptomycin 1) Inhibits beta-subunit of
RNA polymerase
Q) Cycloheximide 2) Inhibits peptidyl
transferase activity of
50S subunit
R) Rifamycin 3) Inhibits peptidyl
transferase activity of
60S subunit
S) Chloramphenicol 4) Inhibits binding of formyl
methionine tRNA to
ribosome
(A) P-1, Q-3, R-4, S-2
(B) P-4, Q-3, R-1, S-2
(C) P-2, Q-3, R-1, S-4
(D) P-3, Q-4, R-1, S-2
20. The major product formed in the given reaction
ANS. B
21. DNA gyrase can
(A) cut single-stranded DNA
(B) relax supercoiled DNA
(C) introduce negative supercoiling in DNA
(D) not utilize ATP
22. The stationary phase of cation-exchange
chromatography can be
(A) DEAE-cellulose
(B) CM-cellulose
(C) Sephadex G-50
(D) Heparin-Sepharose
23. Components of a Transmission Electron
Microscope are
(A) Electron gun, objective lens, positron beam,
projector lens
(B) Neutron beam, projector lens, objective lens,
evacuated tube
(C) Electron beam, projector lens, objective lens,
condenser lens
(D) X-ray beam, projector lens, objective lens,
condenser lens
IIT JAM BIOTECHNOLOGY 2022
24. In a honey bee population, the workers are
infertile but protect the queen from intruders and
help in reproduction. This is an example of
(A) K selection
(B) Sexual selection
(C) Kin selection
(D) Disruptive selection
25. For an enzyme following Michaelis-Menten
kinetics, when [S]=KM then, the velocity v is
([S] is substrate concentration, K M is Michaelis
constant, V max is maximal velocity)
(A) [S] × V max
(B) 0.75 × V max
(C) 0.5 × V
max
(D) K M × V max
26. The net equation for aerobic glycolysis is
(A) Glucose+2ATP? 2 lactate+2ADP+2Pi
(B) Glucose+2ADP+2Pi+2NAD
+
? 2
pyruvate+2ATP+2NADH+2H2O+4H
+
(C) Glucose+2ADP+2Pi ?2 pyruvate+2ATP+2H 2O
(D) Glucose+2ADP+2Pi+2NAD
+
? 2
lactate+2ATP+2NADH+2H 2O+4H
+
27. In the electron transport chain, flavin
mononucleotide (FMN) can adopt ______ as the
highest oxidation state and is capable of accepting
or donating _____ electrons, respectively.
(A) 2; 2 or 3
(B) 2; 1 or 2
(C) 3; 2 or 3
(D) 3; 1 or 2
28. In bacteria, the s factor that plays a major role in
transcription during the stationary phase is
(A) s
70
(B) s
54
(C) s
28
(D) s
3
29. A rise in cytosolic calcium ion concentration just
after fertilization in a sea urchin egg leads to
(A) Formation of fertilization envelope
(B) Acrosomal reaction
(C) Formation of vegetal pole
(D) Formation of animal pole
30. In a nephron, _____ follows the ascending limb of
the “loop of Henle”.
(A) Descending limb (B) Distal tubule
(C) Collecting tubule (D) Proximal tubule
Section B: Q.31 – Q.40 Carry TWO marks each.
31. Transpirational pull that extends down to the
roots in plants can be interrupted by
(A) Process of cavitation
(B) Process of gravitation
(C) Formation of water vapor pockets
(D) Positive pressure in xylem sap
32. Transfer of plasmids into animal cells can be
achieved by
(A) Electroporation
(B) Liposome-mediated process
(C) Calcium chloride treatment
(D) Sucrose treatment
33. Archaeal cell membranes contain lipids that are
(A) Ether linked
(B) Ester linked
(C) Branched alkyl chain
(D) Linear alkyl chain
34. Which of the following are producers in an
ecological system?
(A) Macrophytes
(B) Phytoplanktons
(C) Zooplanktons
(D) Cyanobacteria
35. Which of the following acts as wound hormones
in plants?
(A) Ethylene
(B) Cytokinins
(C) Abscisic acid
(D) Dextrin
36. The enriched media used to facilitate the growth
of fastidious microorganisms are
(A) Selenite F broth
(B) Blood agar
(C) Chocolate agar
(D) Loeffler’s serum
37. Match the bacterial structure to function
Page 4
IIT JAM BIOTECHNOLOGY 2022
QUESTION
Section A: Q.1 – Q.10 Carry ONE mark each.
1. Which of the following is involved in innate
immune response in higher mammals?
(A) T cell antigen receptor
(B) B cell antigen receptor
(C) Toll-like receptor
(D) Major histocompatibility complex-II molecule
2. Which among the following belongs to the family
“Retroviridae”?
(A) Human Immunodeficiency virus
(B) Ebola virus
(C) Dengue virus
(D) Influenza virus
3. Which of the following is a glycolipid?
(A) Cerebroside
(B) Phosphatidylcholine
(C) Phosphatidylserine
(D) Cardiolipin
4. Which of the following bacterial component
contains “dipicolinic acid”?
(A) Endospore
(B) Capsule
(C) Flagella
(D) Pili
5. The fossilization process in which mineral rich
water penetrates through the pores of
decomposed organic matter is known as _____.
(A) Carbonization
(B) Chemical fossilization
(C) Petrifaction
(D) Microfossilization
6. A random fluctuation in gene frequency is called
(A) Genetic drift
(B) Genetic load
(C) Panmixis
(D) Genetic shift
7. The number of “Barr Bodies” present in a somatic
cell of a woman suffering from Turner syndrome
is ____.
(A) 0
(B) 1
(C) 2
(D) 3
8. Which of the following are produced by Mangrove
trees to survive in the waterlogged swampy
forests?
(A) Trichomes
(B) Pneumatophores
(C) Spermatophores
(D) Cambia
9. Indeterminate growth in plants is due to the
presence of perpetually undifferentiated tissues,
called as ______.
(A) Tracheids
(B) Meristems
(C) Parenchyma
(D) Sclerenchyma
10. The osmotic potential (?) of pure water is _____
MPa.
(A) -1
(B) 0
(C) 0.1
(D) 10
Section A: Q.11 – Q.30 Carry TWO marks each.
11. Bacteria containing a tuft of flagella that comes
out from one pole is called ___.
(A) Lophotrichous
(B) Peritrichous
(C) Monotrichous
(D) Amphitrichous
12. Which of the following activity is associated with
Klenow fragment?
(A) 5'-3' exonuclease activity
(B) 5'-3' endonuclease activity
(C) Polymerase activity
(D) 3'-5' endonuclease activity
13. A frameshift mutation is caused by ____.
(A) 5-Bromouracil
(B) Acridine
(C) Glutathione
(D) Hypoxanthine
14. The zone of a pond system where respiration is
more than production is called as _____.
(A) Limnetic zone (B) Littoral zone
(C) Epilimnion zone (D) Benthic zone
IIT JAM BIOTECHNOLOGY 2022
15. An organism that causes obstruction of lymphatic
system in humans is ____.
(A) Borrelia burgdorferi
(B) Brucella abortus
(C) Yersinia pestis
(D) Wuchereria bancrofti
16. A man having a dominant genetic trait (TT
genotype) can taste phenylthiocarbamide (PTC),
marries a woman who cannot taste PTC. The PTC
tasting ability of their biological son and daughter
is
(A) Son taster; Daughter non-taster
(B) Daughter taster; Son non-taster
(C) Both are non-tasters
(D) Both are tasters
17. Which of the following enzymes is absent in a
person suffering from Alkaptonuria?
(A) Tyrosinase
(B) Homogentisic acid oxidase
(C) Catechol dioxygenase
(D) Phenylalanine hydroxylase
18. The bacterium that can tolerate high
concentrations of salt and also ferment mannitol
is
(A) Staphylococcus aureus
(B) Staphylococcus epidermis
(C) Streptococcus pyogenes
(D) Serratia marcescens
19. Match the following
Group I Group II
P) Streptomycin 1) Inhibits beta-subunit of
RNA polymerase
Q) Cycloheximide 2) Inhibits peptidyl
transferase activity of
50S subunit
R) Rifamycin 3) Inhibits peptidyl
transferase activity of
60S subunit
S) Chloramphenicol 4) Inhibits binding of formyl
methionine tRNA to
ribosome
(A) P-1, Q-3, R-4, S-2
(B) P-4, Q-3, R-1, S-2
(C) P-2, Q-3, R-1, S-4
(D) P-3, Q-4, R-1, S-2
20. The major product formed in the given reaction
ANS. B
21. DNA gyrase can
(A) cut single-stranded DNA
(B) relax supercoiled DNA
(C) introduce negative supercoiling in DNA
(D) not utilize ATP
22. The stationary phase of cation-exchange
chromatography can be
(A) DEAE-cellulose
(B) CM-cellulose
(C) Sephadex G-50
(D) Heparin-Sepharose
23. Components of a Transmission Electron
Microscope are
(A) Electron gun, objective lens, positron beam,
projector lens
(B) Neutron beam, projector lens, objective lens,
evacuated tube
(C) Electron beam, projector lens, objective lens,
condenser lens
(D) X-ray beam, projector lens, objective lens,
condenser lens
IIT JAM BIOTECHNOLOGY 2022
24. In a honey bee population, the workers are
infertile but protect the queen from intruders and
help in reproduction. This is an example of
(A) K selection
(B) Sexual selection
(C) Kin selection
(D) Disruptive selection
25. For an enzyme following Michaelis-Menten
kinetics, when [S]=KM then, the velocity v is
([S] is substrate concentration, K M is Michaelis
constant, V max is maximal velocity)
(A) [S] × V max
(B) 0.75 × V max
(C) 0.5 × V
max
(D) K M × V max
26. The net equation for aerobic glycolysis is
(A) Glucose+2ATP? 2 lactate+2ADP+2Pi
(B) Glucose+2ADP+2Pi+2NAD
+
? 2
pyruvate+2ATP+2NADH+2H2O+4H
+
(C) Glucose+2ADP+2Pi ?2 pyruvate+2ATP+2H 2O
(D) Glucose+2ADP+2Pi+2NAD
+
? 2
lactate+2ATP+2NADH+2H 2O+4H
+
27. In the electron transport chain, flavin
mononucleotide (FMN) can adopt ______ as the
highest oxidation state and is capable of accepting
or donating _____ electrons, respectively.
(A) 2; 2 or 3
(B) 2; 1 or 2
(C) 3; 2 or 3
(D) 3; 1 or 2
28. In bacteria, the s factor that plays a major role in
transcription during the stationary phase is
(A) s
70
(B) s
54
(C) s
28
(D) s
3
29. A rise in cytosolic calcium ion concentration just
after fertilization in a sea urchin egg leads to
(A) Formation of fertilization envelope
(B) Acrosomal reaction
(C) Formation of vegetal pole
(D) Formation of animal pole
30. In a nephron, _____ follows the ascending limb of
the “loop of Henle”.
(A) Descending limb (B) Distal tubule
(C) Collecting tubule (D) Proximal tubule
Section B: Q.31 – Q.40 Carry TWO marks each.
31. Transpirational pull that extends down to the
roots in plants can be interrupted by
(A) Process of cavitation
(B) Process of gravitation
(C) Formation of water vapor pockets
(D) Positive pressure in xylem sap
32. Transfer of plasmids into animal cells can be
achieved by
(A) Electroporation
(B) Liposome-mediated process
(C) Calcium chloride treatment
(D) Sucrose treatment
33. Archaeal cell membranes contain lipids that are
(A) Ether linked
(B) Ester linked
(C) Branched alkyl chain
(D) Linear alkyl chain
34. Which of the following are producers in an
ecological system?
(A) Macrophytes
(B) Phytoplanktons
(C) Zooplanktons
(D) Cyanobacteria
35. Which of the following acts as wound hormones
in plants?
(A) Ethylene
(B) Cytokinins
(C) Abscisic acid
(D) Dextrin
36. The enriched media used to facilitate the growth
of fastidious microorganisms are
(A) Selenite F broth
(B) Blood agar
(C) Chocolate agar
(D) Loeffler’s serum
37. Match the bacterial structure to function
IIT JAM BIOTECHNOLOGY 2022
(i) Cell wall
(a) Virulence factor
(ii) Glycocalyx
(b) Selective permeability
(c) Attachment to surfaces
(d) Protection from osmotic
lysis
(A) (i)-(b), (ii)-(d) (B) (i)-(d), (ii)-(a)
(C) (i)-(c), (ii)-(b) (D) (i)-(d), (ii)-(c)
38. Identify the correct pairs:
(i) Thermophile (a) grows optimal at 37 °C
(ii) Mesophile (b) grows optimal at low
temperature
(iii) Psychrophile (c) grows optimal at high
saline conditions
(iv) Halophile (d) grows optimal at 67 °C
(A) (i)-(d)
(B) (ii)-(b)
(C) (iii)-(a)
(D) (iv)-(c)
39. A single copy of an allele in sickle-cell
heterozygous individuals reduces the frequency
and severity of malaria. The reason for this is
(A) Low oxygen binding capacity of hemoglobin
(B) Single amino acid substitution in hemoglobin
deforms the red blood cells
(C) Abnormal hemoglobin is toxic for malaria
parasite
(D) Malaria parasite escapes the deformed red
blood cells
40. The correct statement/s for bimolecular
nucleophilic substitution reactions is/are
(A) It goes through a carbocation formation
(B) There is an inversion of configuration if the
reacting center is chiral
(C) Reaction is enhanced when carried out in polar
solvents
(D) The reaction intermediate is trigonal
bipyramidal
Section C: Q.41 – Q.50 Carry ONE mark each.
41. A deck of ten cards is given to you as shown below
in the figure. One card is drawn at random from
this deck. The probability of selecting a number
less than 9 is ____. (to one decimal place)
ANS. 0.8
42. The average of all positive even integers less than
or equal to 40 is ___.
ANS. 21
43. The smallest positive (non-zero) integer “n” for
which the expression (
1+?? 1-?? )
?? = 1 holds true, is
__________.
ANS. 4
44. Given that
?? = (??????? ??????? ??????? + ??????? ??????? ???????) , the
value of A is
ANS. 1
45. An object is placed at the principal focus of a
concave lens of focal length 10 cm. The image will
be formed at _____cm, between the optical
center and the focus of the lens on the same side
of the object.
ANS. 5
46. What is the maximum number of hydrogen bonds
that a water molecule can make in the liquid
state?
ANS. 4
47. How many pairs of autosomal chromosomes are
there in normal humans?
ANS. 22
48. Calculate the temperature (in K) at which the
resistance of a metal becomes 20% more than its
resistance at 300 K. The value of the temperature
coefficient of resistance for this metal is 2.0 × 10
-
4
/K.
ANS. 1300
Page 5
IIT JAM BIOTECHNOLOGY 2022
QUESTION
Section A: Q.1 – Q.10 Carry ONE mark each.
1. Which of the following is involved in innate
immune response in higher mammals?
(A) T cell antigen receptor
(B) B cell antigen receptor
(C) Toll-like receptor
(D) Major histocompatibility complex-II molecule
2. Which among the following belongs to the family
“Retroviridae”?
(A) Human Immunodeficiency virus
(B) Ebola virus
(C) Dengue virus
(D) Influenza virus
3. Which of the following is a glycolipid?
(A) Cerebroside
(B) Phosphatidylcholine
(C) Phosphatidylserine
(D) Cardiolipin
4. Which of the following bacterial component
contains “dipicolinic acid”?
(A) Endospore
(B) Capsule
(C) Flagella
(D) Pili
5. The fossilization process in which mineral rich
water penetrates through the pores of
decomposed organic matter is known as _____.
(A) Carbonization
(B) Chemical fossilization
(C) Petrifaction
(D) Microfossilization
6. A random fluctuation in gene frequency is called
(A) Genetic drift
(B) Genetic load
(C) Panmixis
(D) Genetic shift
7. The number of “Barr Bodies” present in a somatic
cell of a woman suffering from Turner syndrome
is ____.
(A) 0
(B) 1
(C) 2
(D) 3
8. Which of the following are produced by Mangrove
trees to survive in the waterlogged swampy
forests?
(A) Trichomes
(B) Pneumatophores
(C) Spermatophores
(D) Cambia
9. Indeterminate growth in plants is due to the
presence of perpetually undifferentiated tissues,
called as ______.
(A) Tracheids
(B) Meristems
(C) Parenchyma
(D) Sclerenchyma
10. The osmotic potential (?) of pure water is _____
MPa.
(A) -1
(B) 0
(C) 0.1
(D) 10
Section A: Q.11 – Q.30 Carry TWO marks each.
11. Bacteria containing a tuft of flagella that comes
out from one pole is called ___.
(A) Lophotrichous
(B) Peritrichous
(C) Monotrichous
(D) Amphitrichous
12. Which of the following activity is associated with
Klenow fragment?
(A) 5'-3' exonuclease activity
(B) 5'-3' endonuclease activity
(C) Polymerase activity
(D) 3'-5' endonuclease activity
13. A frameshift mutation is caused by ____.
(A) 5-Bromouracil
(B) Acridine
(C) Glutathione
(D) Hypoxanthine
14. The zone of a pond system where respiration is
more than production is called as _____.
(A) Limnetic zone (B) Littoral zone
(C) Epilimnion zone (D) Benthic zone
IIT JAM BIOTECHNOLOGY 2022
15. An organism that causes obstruction of lymphatic
system in humans is ____.
(A) Borrelia burgdorferi
(B) Brucella abortus
(C) Yersinia pestis
(D) Wuchereria bancrofti
16. A man having a dominant genetic trait (TT
genotype) can taste phenylthiocarbamide (PTC),
marries a woman who cannot taste PTC. The PTC
tasting ability of their biological son and daughter
is
(A) Son taster; Daughter non-taster
(B) Daughter taster; Son non-taster
(C) Both are non-tasters
(D) Both are tasters
17. Which of the following enzymes is absent in a
person suffering from Alkaptonuria?
(A) Tyrosinase
(B) Homogentisic acid oxidase
(C) Catechol dioxygenase
(D) Phenylalanine hydroxylase
18. The bacterium that can tolerate high
concentrations of salt and also ferment mannitol
is
(A) Staphylococcus aureus
(B) Staphylococcus epidermis
(C) Streptococcus pyogenes
(D) Serratia marcescens
19. Match the following
Group I Group II
P) Streptomycin 1) Inhibits beta-subunit of
RNA polymerase
Q) Cycloheximide 2) Inhibits peptidyl
transferase activity of
50S subunit
R) Rifamycin 3) Inhibits peptidyl
transferase activity of
60S subunit
S) Chloramphenicol 4) Inhibits binding of formyl
methionine tRNA to
ribosome
(A) P-1, Q-3, R-4, S-2
(B) P-4, Q-3, R-1, S-2
(C) P-2, Q-3, R-1, S-4
(D) P-3, Q-4, R-1, S-2
20. The major product formed in the given reaction
ANS. B
21. DNA gyrase can
(A) cut single-stranded DNA
(B) relax supercoiled DNA
(C) introduce negative supercoiling in DNA
(D) not utilize ATP
22. The stationary phase of cation-exchange
chromatography can be
(A) DEAE-cellulose
(B) CM-cellulose
(C) Sephadex G-50
(D) Heparin-Sepharose
23. Components of a Transmission Electron
Microscope are
(A) Electron gun, objective lens, positron beam,
projector lens
(B) Neutron beam, projector lens, objective lens,
evacuated tube
(C) Electron beam, projector lens, objective lens,
condenser lens
(D) X-ray beam, projector lens, objective lens,
condenser lens
IIT JAM BIOTECHNOLOGY 2022
24. In a honey bee population, the workers are
infertile but protect the queen from intruders and
help in reproduction. This is an example of
(A) K selection
(B) Sexual selection
(C) Kin selection
(D) Disruptive selection
25. For an enzyme following Michaelis-Menten
kinetics, when [S]=KM then, the velocity v is
([S] is substrate concentration, K M is Michaelis
constant, V max is maximal velocity)
(A) [S] × V max
(B) 0.75 × V max
(C) 0.5 × V
max
(D) K M × V max
26. The net equation for aerobic glycolysis is
(A) Glucose+2ATP? 2 lactate+2ADP+2Pi
(B) Glucose+2ADP+2Pi+2NAD
+
? 2
pyruvate+2ATP+2NADH+2H2O+4H
+
(C) Glucose+2ADP+2Pi ?2 pyruvate+2ATP+2H 2O
(D) Glucose+2ADP+2Pi+2NAD
+
? 2
lactate+2ATP+2NADH+2H 2O+4H
+
27. In the electron transport chain, flavin
mononucleotide (FMN) can adopt ______ as the
highest oxidation state and is capable of accepting
or donating _____ electrons, respectively.
(A) 2; 2 or 3
(B) 2; 1 or 2
(C) 3; 2 or 3
(D) 3; 1 or 2
28. In bacteria, the s factor that plays a major role in
transcription during the stationary phase is
(A) s
70
(B) s
54
(C) s
28
(D) s
3
29. A rise in cytosolic calcium ion concentration just
after fertilization in a sea urchin egg leads to
(A) Formation of fertilization envelope
(B) Acrosomal reaction
(C) Formation of vegetal pole
(D) Formation of animal pole
30. In a nephron, _____ follows the ascending limb of
the “loop of Henle”.
(A) Descending limb (B) Distal tubule
(C) Collecting tubule (D) Proximal tubule
Section B: Q.31 – Q.40 Carry TWO marks each.
31. Transpirational pull that extends down to the
roots in plants can be interrupted by
(A) Process of cavitation
(B) Process of gravitation
(C) Formation of water vapor pockets
(D) Positive pressure in xylem sap
32. Transfer of plasmids into animal cells can be
achieved by
(A) Electroporation
(B) Liposome-mediated process
(C) Calcium chloride treatment
(D) Sucrose treatment
33. Archaeal cell membranes contain lipids that are
(A) Ether linked
(B) Ester linked
(C) Branched alkyl chain
(D) Linear alkyl chain
34. Which of the following are producers in an
ecological system?
(A) Macrophytes
(B) Phytoplanktons
(C) Zooplanktons
(D) Cyanobacteria
35. Which of the following acts as wound hormones
in plants?
(A) Ethylene
(B) Cytokinins
(C) Abscisic acid
(D) Dextrin
36. The enriched media used to facilitate the growth
of fastidious microorganisms are
(A) Selenite F broth
(B) Blood agar
(C) Chocolate agar
(D) Loeffler’s serum
37. Match the bacterial structure to function
IIT JAM BIOTECHNOLOGY 2022
(i) Cell wall
(a) Virulence factor
(ii) Glycocalyx
(b) Selective permeability
(c) Attachment to surfaces
(d) Protection from osmotic
lysis
(A) (i)-(b), (ii)-(d) (B) (i)-(d), (ii)-(a)
(C) (i)-(c), (ii)-(b) (D) (i)-(d), (ii)-(c)
38. Identify the correct pairs:
(i) Thermophile (a) grows optimal at 37 °C
(ii) Mesophile (b) grows optimal at low
temperature
(iii) Psychrophile (c) grows optimal at high
saline conditions
(iv) Halophile (d) grows optimal at 67 °C
(A) (i)-(d)
(B) (ii)-(b)
(C) (iii)-(a)
(D) (iv)-(c)
39. A single copy of an allele in sickle-cell
heterozygous individuals reduces the frequency
and severity of malaria. The reason for this is
(A) Low oxygen binding capacity of hemoglobin
(B) Single amino acid substitution in hemoglobin
deforms the red blood cells
(C) Abnormal hemoglobin is toxic for malaria
parasite
(D) Malaria parasite escapes the deformed red
blood cells
40. The correct statement/s for bimolecular
nucleophilic substitution reactions is/are
(A) It goes through a carbocation formation
(B) There is an inversion of configuration if the
reacting center is chiral
(C) Reaction is enhanced when carried out in polar
solvents
(D) The reaction intermediate is trigonal
bipyramidal
Section C: Q.41 – Q.50 Carry ONE mark each.
41. A deck of ten cards is given to you as shown below
in the figure. One card is drawn at random from
this deck. The probability of selecting a number
less than 9 is ____. (to one decimal place)
ANS. 0.8
42. The average of all positive even integers less than
or equal to 40 is ___.
ANS. 21
43. The smallest positive (non-zero) integer “n” for
which the expression (
1+?? 1-?? )
?? = 1 holds true, is
__________.
ANS. 4
44. Given that
?? = (??????? ??????? ??????? + ??????? ??????? ???????) , the
value of A is
ANS. 1
45. An object is placed at the principal focus of a
concave lens of focal length 10 cm. The image will
be formed at _____cm, between the optical
center and the focus of the lens on the same side
of the object.
ANS. 5
46. What is the maximum number of hydrogen bonds
that a water molecule can make in the liquid
state?
ANS. 4
47. How many pairs of autosomal chromosomes are
there in normal humans?
ANS. 22
48. Calculate the temperature (in K) at which the
resistance of a metal becomes 20% more than its
resistance at 300 K. The value of the temperature
coefficient of resistance for this metal is 2.0 × 10
-
4
/K.
ANS. 1300
IIT JAM BIOTECHNOLOGY 2022
49. In the
1
H NMR spectrum of ethanol at 400 MHz,
the methyl group splits into ___ number of peaks.
ANS.3
50. In a denaturing polyacrylamide gel
electrophoresis experiment, pure intact adult
human hemoglobin will yield ________. (number)
bands.
ANS. 2
Section C: Q.51 – Q.60 Carry TWO marks each.
51. A man throws a ball vertically up in the air with an
initial velocity v 1 such that it reaches a height of
12 m with a speed of 12 m/s. If he throws the
same ball vertically up with an initial velocity v
2
such that it reaches a maximum height of 12 m.
Calculate v 1/v 2. (up to 2 decimal places)
ANS. 1.26
52. What is the acceleration due to gravity (m/s
2
) on
the surface of a planet if its radius is 1/4
th
that of
earth and its mass is 1/80
th
that of earth? Assume
that the gravity on the surface of the earth is 10
m/s
2
.
ANS. 2
53. In a randomly mating population, the frequency
of ‘A’ allele is 0.7. What is the frequency of ‘Aa’
genotype in the next generation according to
Hardy-Weinberg’s law? (up to two decimal
places)
ANS. 0.42
54. The potential difference to accelerate an electron
was quadrupled. By what factor does the De
Broglie wavelength of the electron beam change?
ANS. 0.5
55. A 500 nm light is used for imaging in a confocal
microscope. What will be the best resolution (in
nm) of this microscope?
ANS. 203.33
56. Assuming the molecule shown below is aromatic,
the value of “n” according to “Hückel’s rule” is
ANS. 3
57. In an actively growing population from a single
bacterium, 1,048,576 cells are present after 20
th
generation. How many cells were there in 5
th
generation?
ANS. 32
58. A double stranded DNA molecule of total 5000
base pairs long, has a melting temperature of 85
°C. What will be the % AT base pairs in this
sample? (up to one decimal place).
ANS. 50.74
59. How many GTP molecules are required for the
translocation of t-RNA from P site to E site during
translation elongation process in bacteria?
ANS. 1
60. Amongst the molecules given below, the total
number of molecules that have at least one sp
2
hybridized atom is __________.
C 6H 6, NO 2, BF 3, H 2O 2, SO 2, C 2H 2, L-Tryptophan
ANS. 5
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