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A reagent suitable for the determination of Nterminal residue of a peptide is [1996]
- a)p-toluenesulphonyl chloride
- b)2, 4-dinitrophenylhydrazine
- c)carboxypeptidase
- d)2, 4-dinitrofluorobenzene
Correct answer is option 'D'. Can you explain this answer?
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A reagent suitable for the determination of Nterminal residue of a pep...
2, 4-dinitro fluorobenzene is called Sanger- reagent. When this reagent reacts with amino group of peptide chain, it form 2,4-dinitrophenyl derivatives which on hydrolysis form DNP derivatives of amino acids.
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A reagent suitable for the determination of Nterminal residue of a pep...
Reagents for the determination of N-terminal residue of a peptide
To determine the N-terminal residue of a peptide, several reagents can be used. Among the options given, the correct answer is option D, 2, 4-dinitrofluorobenzene.
1. p-toluenesulphonyl chloride (option A)
p-toluenesulphonyl chloride, also known as tosyl chloride, is commonly used for the protection of amine groups in peptide synthesis. However, it is not suitable for the determination of the N-terminal residue of a peptide.
2. 2, 4-dinitrophenylhydrazine (option B)
2, 4-dinitrophenylhydrazine (DNPH) is a reagent commonly used for the identification of carbonyl compounds. It forms a yellow to orange precipitate with carbonyl-containing compounds, such as aldehydes and ketones. While it can react with the N-terminal residue of a peptide, it is not specific for this purpose and can react with other functional groups as well.
3. Carboxypeptidase (option C)
Carboxypeptidase is an enzyme that catalyzes the hydrolysis of the C-terminal amino acid residue from a peptide or protein. It is not suitable for the determination of the N-terminal residue of a peptide as it acts on the opposite end.
4. 2, 4-dinitrofluorobenzene (option D)
2, 4-dinitrofluorobenzene (DNFB) is a reagent specifically used for the determination of the N-terminal residue of a peptide. It reacts with the primary amine group present at the N-terminus of a peptide to form a stable derivative. This reaction is known as the Sanger's reagent method and is widely used for the sequencing of peptides.
In conclusion, among the given options, 2, 4-dinitrofluorobenzene (option D) is the most suitable reagent for the determination of the N-terminal residue of a peptide.
To determine the N-terminal residue of a peptide, several reagents can be used. Among the options given, the correct answer is option D, 2, 4-dinitrofluorobenzene.
1. p-toluenesulphonyl chloride (option A)
p-toluenesulphonyl chloride, also known as tosyl chloride, is commonly used for the protection of amine groups in peptide synthesis. However, it is not suitable for the determination of the N-terminal residue of a peptide.
2. 2, 4-dinitrophenylhydrazine (option B)
2, 4-dinitrophenylhydrazine (DNPH) is a reagent commonly used for the identification of carbonyl compounds. It forms a yellow to orange precipitate with carbonyl-containing compounds, such as aldehydes and ketones. While it can react with the N-terminal residue of a peptide, it is not specific for this purpose and can react with other functional groups as well.
3. Carboxypeptidase (option C)
Carboxypeptidase is an enzyme that catalyzes the hydrolysis of the C-terminal amino acid residue from a peptide or protein. It is not suitable for the determination of the N-terminal residue of a peptide as it acts on the opposite end.
4. 2, 4-dinitrofluorobenzene (option D)
2, 4-dinitrofluorobenzene (DNFB) is a reagent specifically used for the determination of the N-terminal residue of a peptide. It reacts with the primary amine group present at the N-terminus of a peptide to form a stable derivative. This reaction is known as the Sanger's reagent method and is widely used for the sequencing of peptides.
In conclusion, among the given options, 2, 4-dinitrofluorobenzene (option D) is the most suitable reagent for the determination of the N-terminal residue of a peptide.
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A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer?
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A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer? for Class 12 2024 is part of Class 12 preparation. The Question and answers have been prepared according to the Class 12 exam syllabus. Information about A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for Class 12 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer?.
A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer? for Class 12 2024 is part of Class 12 preparation. The Question and answers have been prepared according to the Class 12 exam syllabus. Information about A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for Class 12 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A reagent suitable for the determination of Nterminal residue of a peptide is [1996]a)p-toluenesulphonyl chlorideb)2, 4-dinitrophenylhydrazinec)carboxypeptidased)2, 4-dinitrofluorobenzeneCorrect answer is option 'D'. Can you explain this answer?.
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