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An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol–1 cm–1 for an absorbance value of 0.1 at 540 nm (Given : cell thickness = 1 cm). The concentration (in μM) of the hemoglobin solution is
  • a)
    0.537
  • b)
    5.37
  • c)
    53.7
  • d)
    537.0
Correct answer is option 'B'. Can you explain this answer?
Most Upvoted Answer
An aqueous solution of hemoglobin has a molar absorptivity value of 18...
Given parameters:

Molar absorptivity value (ε) = 18,600 L mol^-1 cm^-1

Absorbance (A) = 0.1

Wavelength (λ) = 540 nm

Cell thickness (b) = 1 cm

We can use the Beer-Lambert law to calculate the concentration (c) of the hemoglobin solution.

Beer-Lambert law states that the absorbance (A) of a solution is directly proportional to the concentration (c) and the path length (b) of the solution:

A = εbc

Rearranging this formula, we get:

c = A/ (εb)

Substituting the given values, we get:

c = 0.1 / (18,600 L mol^-1 cm^-1 x 1 cm)

c = 0.00000537 mol/L

Converting the units to M, we get:

c = 5.37 x 10^-6 M

Therefore, the concentration of the hemoglobin solution is 5.37 x 10^-6 M, which is equivalent to option B.
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An aqueous solution of hemoglobin has a molar absorptivity value of 18...
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An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol–1 cm–1 for an absorbance value of 0.1 at 540 nm (Given : cell thickness = 1 cm). The concentration (in μM) of the hemoglobin solution isa)0.537b)5.37c)53.7d)537.0Correct answer is option 'B'. Can you explain this answer?
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An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol–1 cm–1 for an absorbance value of 0.1 at 540 nm (Given : cell thickness = 1 cm). The concentration (in μM) of the hemoglobin solution isa)0.537b)5.37c)53.7d)537.0Correct answer is option 'B'. Can you explain this answer? for Chemistry 2024 is part of Chemistry preparation. The Question and answers have been prepared according to the Chemistry exam syllabus. Information about An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol–1 cm–1 for an absorbance value of 0.1 at 540 nm (Given : cell thickness = 1 cm). The concentration (in μM) of the hemoglobin solution isa)0.537b)5.37c)53.7d)537.0Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for Chemistry 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for An aqueous solution of hemoglobin has a molar absorptivity value of 18,600 L mol–1 cm–1 for an absorbance value of 0.1 at 540 nm (Given : cell thickness = 1 cm). The concentration (in μM) of the hemoglobin solution isa)0.537b)5.37c)53.7d)537.0Correct answer is option 'B'. Can you explain this answer?.
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