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Which of the following is TRUE when cations are added to a solution of DNA molecules?
  • a)
    Increases the Tm
  • b)
    Decreases the Tm
  • c)
    Does not affect the Tm   
  • d)
    First, it increases and then decreases Tm
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
Which of the following is TRUE when cations are added to a solution of...
When the cations are added to DNA solution, the melting temperature (Tm) of DNA increase. The reason for this is that cations have positive charge, which bind to the negative phosphate backbone of DNA, thereby shielding the negative charge, due to which DNA double helix is stabilized and its Tm increases.
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Which of the following is TRUE when cations are added to a solution of DNA molecules?a)Increases the Tmb)Decreases the Tmc)Does not affect the Tmd)First, it increases and then decreases TmCorrect answer is option 'A'. Can you explain this answer?
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