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A relaxed circular plasmid has linking number 360. The plasmid is transformed in  E. coli cells  and re-isolated. The newly isolated plasmid was found to be supercoiled negatively by +60 writhe value.  The total size of the plasmid is 3600 bp. What is the number of base pairs per turn in the new plasmid? ____________ (answer in integer)
    Correct answer is '12'. Can you explain this answer?
    Verified Answer
    A relaxed circular plasmid has linking number 360. The plasmid is tran...
    Linking no. (Lk) = Twist + writhe        ... (1)
    The Lk of the circular DNA remains unchanged if the DNA does not break and re-joins again. Since, in this case also there is no cutting and rejoining, so the Lk of the new plasmid will remain same i.e. 360
    Also, Writhe = +60
    Substituting the values of linking number and writhe in equation (1), we get 360 = twist + 60; New twist = 360 -60 = 300.
    No. of base pairs per helix turn in the new plasmid = 3600/300 =12
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    A relaxed circular plasmid has linking number 360. The plasmid is transformed in E. coli cells and re-isolated. The newly isolated plasmid was found to be supercoiled negatively by +60 writhe value. The total size of the plasmid is 3600 bp. What is the number of base pairs per turn in the new plasmid? ____________ (answer in integer)Correct answer is '12'. Can you explain this answer?
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    A relaxed circular plasmid has linking number 360. The plasmid is transformed in E. coli cells and re-isolated. The newly isolated plasmid was found to be supercoiled negatively by +60 writhe value. The total size of the plasmid is 3600 bp. What is the number of base pairs per turn in the new plasmid? ____________ (answer in integer)Correct answer is '12'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about A relaxed circular plasmid has linking number 360. The plasmid is transformed in E. coli cells and re-isolated. The newly isolated plasmid was found to be supercoiled negatively by +60 writhe value. The total size of the plasmid is 3600 bp. What is the number of base pairs per turn in the new plasmid? ____________ (answer in integer)Correct answer is '12'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A relaxed circular plasmid has linking number 360. The plasmid is transformed in E. coli cells and re-isolated. The newly isolated plasmid was found to be supercoiled negatively by +60 writhe value. The total size of the plasmid is 3600 bp. What is the number of base pairs per turn in the new plasmid? ____________ (answer in integer)Correct answer is '12'. Can you explain this answer?.
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