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Which of the following is true about nucleic acid
- a)Palindromic DNA sequences can form hairpin or cruciforms
- b)N-7, O6 and N6 of purines are referred to as Hoogsteen positions
- c)Denaturation of double stranded nucleic acid results in increase in absorption
- d)GC rich dsDNA segment will have higher Tm, than AT rich dsDNA
Correct answer is option 'A,B,C,D'. Can you explain this answer?
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Verified Answer
Which of the following is true about nucleic acida)Palindromic DNA seq...
Option (a) is correct.
Palindromic DNA/RNA sequences can form alternative structures with intrastrand base pairing. When only single DNA/RNA strand is involved, hairpin structure is formed. When both strands of duplex DNA are involved, it is called cruciform.
Option (b) is correct.
N-7, O6 and N6 of purines are the atoms that participate in the hydrogen bonding of triplex DNA resulting in Hoogsteen pairing, thereby forming triplex DNAs.
Option (c) is correct.
Increase in absorption that accompanies the denaturation of double stranded nucleic acid is called hyper chromic effect which can be measured by monitoring absorption of UV light.
Option (d) is correct.
GC pair has 3 hydrogen bonds unlike AT pair which has 2 hydrogen bond. It is easier to break 2 hydrogen bond than 3.
Palindromic DNA/RNA sequences can form alternative structures with intrastrand base pairing. When only single DNA/RNA strand is involved, hairpin structure is formed. When both strands of duplex DNA are involved, it is called cruciform.
Option (b) is correct.
N-7, O6 and N6 of purines are the atoms that participate in the hydrogen bonding of triplex DNA resulting in Hoogsteen pairing, thereby forming triplex DNAs.
Option (c) is correct.
Increase in absorption that accompanies the denaturation of double stranded nucleic acid is called hyper chromic effect which can be measured by monitoring absorption of UV light.
Option (d) is correct.
GC pair has 3 hydrogen bonds unlike AT pair which has 2 hydrogen bond. It is easier to break 2 hydrogen bond than 3.
Most Upvoted Answer
Which of the following is true about nucleic acida)Palindromic DNA seq...
Palindromic DNA sequences can form hairpin or cruciforms:
- Palindromic DNA sequences are sequences that read the same on both strands when read in the 5' to 3' direction.
- These sequences can form secondary structures such as hairpin loops or cruciforms through intrastrand base pairing.
- Hairpin loops are formed when the palindromic sequence folds back on itself, creating a stem-loop structure.
- Cruciforms are formed when the palindromic sequence forms a four-armed structure through intrastrand base pairing.
N-7, O6, and N6 of purines are referred to as Hoogsteen positions:
- N-7, O6, and N6 are specific nitrogen and oxygen atoms on purine bases (adenine and guanine).
- These positions are referred to as Hoogsteen positions because they are involved in non-canonical base pairing interactions.
- In Hoogsteen base pairing, the purine base rotates around the glycosidic bond to form hydrogen bonds with another base, resulting in non-standard hydrogen bonding interactions.
Denaturation of double-stranded nucleic acid results in an increase in absorption:
- Denaturation is the process of separating the two strands of a double-stranded nucleic acid molecule, such as DNA or RNA.
- This process can be induced by various factors such as heat, pH changes, or chemicals.
- When a double-stranded nucleic acid is denatured, the absorption of UV light increases.
- This is because the stacking interactions between adjacent base pairs in the double helix are disrupted, leading to increased exposure of the bases to UV light and increased absorption.
GC-rich dsDNA segment will have a higher Tm than an AT-rich dsDNA:
- The melting temperature (Tm) is the temperature at which 50% of the double-stranded DNA is denatured.
- GC-rich dsDNA segments have a higher Tm compared to AT-rich dsDNA segments.
- This is because the GC base pair is held together by three hydrogen bonds, while the AT base pair is held together by two hydrogen bonds.
- The additional hydrogen bond in the GC base pair provides greater stability and requires a higher temperature for denaturation.
- Therefore, GC-rich dsDNA segments require a higher temperature (higher Tm) to denature compared to AT-rich dsDNA segments.
- Palindromic DNA sequences are sequences that read the same on both strands when read in the 5' to 3' direction.
- These sequences can form secondary structures such as hairpin loops or cruciforms through intrastrand base pairing.
- Hairpin loops are formed when the palindromic sequence folds back on itself, creating a stem-loop structure.
- Cruciforms are formed when the palindromic sequence forms a four-armed structure through intrastrand base pairing.
N-7, O6, and N6 of purines are referred to as Hoogsteen positions:
- N-7, O6, and N6 are specific nitrogen and oxygen atoms on purine bases (adenine and guanine).
- These positions are referred to as Hoogsteen positions because they are involved in non-canonical base pairing interactions.
- In Hoogsteen base pairing, the purine base rotates around the glycosidic bond to form hydrogen bonds with another base, resulting in non-standard hydrogen bonding interactions.
Denaturation of double-stranded nucleic acid results in an increase in absorption:
- Denaturation is the process of separating the two strands of a double-stranded nucleic acid molecule, such as DNA or RNA.
- This process can be induced by various factors such as heat, pH changes, or chemicals.
- When a double-stranded nucleic acid is denatured, the absorption of UV light increases.
- This is because the stacking interactions between adjacent base pairs in the double helix are disrupted, leading to increased exposure of the bases to UV light and increased absorption.
GC-rich dsDNA segment will have a higher Tm than an AT-rich dsDNA:
- The melting temperature (Tm) is the temperature at which 50% of the double-stranded DNA is denatured.
- GC-rich dsDNA segments have a higher Tm compared to AT-rich dsDNA segments.
- This is because the GC base pair is held together by three hydrogen bonds, while the AT base pair is held together by two hydrogen bonds.
- The additional hydrogen bond in the GC base pair provides greater stability and requires a higher temperature for denaturation.
- Therefore, GC-rich dsDNA segments require a higher temperature (higher Tm) to denature compared to AT-rich dsDNA segments.
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Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer?
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Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer?.
Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is true about nucleic acida)Palindromic DNA sequences can form hairpin or cruciformsb)N-7, O6and N6of purines are referred to as Hoogsteen positionsc)Denaturation of double stranded nucleic acid results in increase in absorptiond)GC rich dsDNA segment will have higher Tm, than AT rich dsDNACorrect answer is option 'A,B,C,D'. Can you explain this answer?.
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