The hybridization of probes having radioactive isotopes with various sequence of nitrogen bases with ssVNTR is called?a)Eastern blottingb)Southern blottingc)Western blottingd)Northern blottingCorrect answer is option 'B'. Can you explain this answer?

Question

Answer

The hybridization of probes having radioactive isotopes with various sequence of nitrogen bases with ssVNTR is called southern blotting. Southern blotting is a laboratory technique used to detect a specific DNA sequence in a blood or tissue sample. A restriction enzyme is used to cut a sample of DNA into fragments that are separated using gel electrophoresis. The DNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag. If the probe binds to the membrane, then the probe sequence is present in the sample. Hence, the correct answer is option 'B'.

Answer

One procedure also done after elution that is spreading DNA fragment on nitrocellulose paper. this process is known as southern blotting.

Answer

Introduction:
The technique used to detect specific DNA sequences in a sample is known as blotting. It involves transferring DNA fragments from a gel onto a membrane and then hybridizing them with complementary probes. The hybridization of probes with a single-stranded Variable Number Tandem Repeat (ssVNTR) sequence using radioactive isotopes is known as Southern blotting.

Southern Blotting:
Southern blotting is a widely used technique in molecular biology that allows the detection of specific DNA sequences. It was named after its inventor, Edwin Southern. This technique involves several steps:

1. DNA Fragmentation: The DNA sample is first digested with restriction enzymes, which cut the DNA at specific recognition sites. This step produces a collection of DNA fragments of various lengths.

2. Gel Electrophoresis: The DNA fragments are then separated based on their size using gel electrophoresis. The fragments are loaded into wells of an agarose gel and subjected to an electric field, causing them to migrate through the gel. Smaller fragments move faster and travel further than larger fragments.

3. Transfer to a Membrane: After gel electrophoresis, the DNA fragments are transferred from the gel onto a membrane, typically made of nitrocellulose or nylon. This transfer is achieved through a process called capillary or electroblotting.

4. Probing: The transferred DNA fragments on the membrane are then hybridized with a probe. The probe is a single-stranded DNA or RNA molecule that is complementary to the target sequence of interest. The probe is typically labeled with a radioactive isotope or a fluorescent molecule for detection.

5. Hybridization: The probe is allowed to hybridize with the complementary DNA sequences on the membrane. If the target sequence is present in the sample, it will form a stable double-stranded DNA structure with the probe.

6. Detection: The radioactive or fluorescent label on the probe allows for the detection of the hybridized DNA fragments. This can be visualized using autoradiography (for radioactive probes) or fluorescence imaging (for fluorescent probes).

Conclusion:
In conclusion, the hybridization of probes labeled with radioactive isotopes with a single-stranded Variable Number Tandem Repeat (ssVNTR) sequence is known as Southern blotting. This technique is commonly used to detect specific DNA sequences and has numerous applications in molecular biology and genetics research.

Answer

The hybridization of probes having radioactive isotopes with various sequence of nitrogen bases with ssVNTR is called Southern blotting.

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