In human genome project, commonly used host were bacteria and yeast an...
BAC = bacterial artificial chromosome
YAC= yeast artificial chromosome... refer ncert class 12 bio..
In human genome project, commonly used host were bacteria and yeast an...
Introduction:
The Human Genome Project (HGP) was a collaborative scientific research project that aimed to sequence and map the entire human genome. It involved the use of various organisms as hosts to clone and replicate DNA fragments, and the vectors used in this process were Bacterial Artificial Chromosomes (BAC) and Yeast Artificial Chromosomes (YAC).
Explanation:
1. Bacterial Artificial Chromosomes (BAC):
- BACs are large DNA molecules that can be used as vectors to carry and replicate DNA fragments.
- They are derived from the F-factor plasmid found in Escherichia coli (E. coli) bacteria.
- BACs have several advantages, including their ability to carry large DNA fragments (up to 300 kilobases) and their stability during replication.
- BACs are commonly used in the Human Genome Project to clone and amplify human DNA fragments.
2. Yeast Artificial Chromosomes (YAC):
- YACs are another type of vector used in the Human Genome Project.
- They are derived from yeast cells, specifically Saccharomyces cerevisiae, a type of yeast commonly used in molecular biology research.
- YACs have the ability to replicate and maintain large DNA fragments (up to 2 million base pairs) in yeast cells.
- YACs also have the advantage of being able to accommodate complex DNA sequences, including those with repetitive elements.
- YACs were extensively used in the Human Genome Project to clone and analyze human DNA fragments.
Summary:
In summary, the commonly used hosts in the Human Genome Project were bacteria, specifically E. coli, and yeast, specifically S. cerevisiae. The vectors used in this project were Bacterial Artificial Chromosomes (BAC) and Yeast Artificial Chromosomes (YAC). These vectors allowed researchers to clone and replicate large DNA fragments, contributing to the successful sequencing and mapping of the human genome.