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Binding of RNA polymerase to its specific promoter in E.coli:-
- a)requires sigma factor
- b)is enhanced by methylation of purine bases
- c)results in temperature dependent unwinding of approximately 10 base pairs.
- d)both (b) and (c)
Correct answer is option 'A,C'. Can you explain this answer?
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Binding of RNA polymerase to its specific promoter in E.coli:-a)requir...
In E.coli, RNA polymerase is recruited onto the promoters of genes with the help of sigma factor, which plays a role in the promoter identification. Near the transcription start site, six nucleotide rich A+T base pair sequence (TATA sequence) is present, which because of its lower melting temperature, initiates the unwinding of ds DNA, so that transcription machinery can act on it
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Binding of RNA polymerase to its specific promoter in E.coli:-a)requir...
Binding of RNA polymerase to its specific promoter in E.coli
Requires sigma factor:
RNA polymerase is the enzyme responsible for transcription in E.coli. In order for RNA polymerase to bind to its specific promoter sequence, it requires the assistance of a sigma factor. The sigma factor is a subunit of RNA polymerase that recognizes and binds to the specific promoter region on the DNA. This binding is essential for the initiation of transcription.
Results in temperature-dependent unwinding of approximately 10 base pairs:
Once the RNA polymerase-sigma factor complex binds to the promoter sequence, it initiates the process of unwinding the DNA double helix. This unwinding is necessary for the RNA polymerase to access the template strand and synthesize the RNA molecule. The unwinding of the DNA helix occurs in a temperature-dependent manner. At higher temperatures, the DNA strands are more easily separated, facilitating the unwinding process.
Enhanced by methylation of purine bases:
Methylation refers to the addition of a methyl group (-CH3) to a molecule. In E.coli, methylation of purine bases (adenine and guanine) on the DNA molecule can enhance the binding of RNA polymerase to its specific promoter. Methylation of certain purine bases within the promoter region can provide additional recognition sites for the sigma factor, thereby increasing the efficiency of binding.
Explanation of correct answer (A, C):
The correct answer is A and C. Binding of RNA polymerase to its specific promoter in E.coli requires the assistance of a sigma factor. The sigma factor recognizes and binds to the promoter region of DNA, allowing RNA polymerase to initiate transcription. Additionally, the binding of RNA polymerase to the promoter results in temperature-dependent unwinding of approximately 10 base pairs. The unwinding of the DNA helix facilitates the access of RNA polymerase to the template strand for transcription. Therefore, the correct answer is A and C.
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Binding of RNA polymerase to its specific promoter in E.coli:-a)requires sigma factorb)is enhanced by methylation of purine basesc)results in temperature dependent unwinding of approximately 10 base pairs.d)both (b) and (c)Correct answer is option 'A,C'. Can you explain this answer?
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