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A restriction site “GAATTC” is recognized by an enzyme EcoRI, How many sites of this sequence are probable in a cloning vector, pBR322 of size 4361 bp.
- a)One
- b)Two
- c)Three
- d)Four
Correct answer is option 'A'. Can you explain this answer?
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Verified Answer
A restriction site “GAATTC” is recognized by an enzyme Eco...
The probability of restriction site in a given DNA is calculated by formula
One in 4n, where n is the number of base pairs in a site
i.e. one site in 4096 bp,hence a DNA of size 4361 is likely to possess only one site based on random probability
One in 4n, where n is the number of base pairs in a site
i.e. one site in 4096 bp,hence a DNA of size 4361 is likely to possess only one site based on random probability
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A restriction site “GAATTC” is recognized by an enzyme Eco...
A restriction site, also known as a recognition site, is a specific DNA sequence where a restriction enzyme can bind and cut the DNA molecule. These sites are usually palindromic sequences, meaning that they read the same forward and backward on complementary strands of DNA. The cutting of DNA at restriction sites is an essential step in many molecular biology techniques, such as DNA cloning and genetic engineering. Different restriction enzymes recognize and cut at different specific sequences, allowing scientists to precisely manipulate and analyze DNA molecules.
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A restriction site “GAATTC” is recognized by an enzyme EcoRI, How many sites of this sequence are probable in a cloning vector, pBR322 of size 4361 bp.a)Oneb)Twoc)Threed)FourCorrect answer is option 'A'. Can you explain this answer?
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Solutions for A restriction site “GAATTC” is recognized by an enzyme EcoRI, How many sites of this sequence are probable in a cloning vector, pBR322 of size 4361 bp.a)Oneb)Twoc)Threed)FourCorrect answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for IIT JAM.
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