Calculation of Minimum Molecular Weight of Peroxidase Enzyme

Peroxidase Enzyme and its Composition

Peroxidase is an enzyme that catalyzes the oxidation of a substrate by hydrogen peroxide. It is a heme-containing enzyme that is found in many organisms, including plants, animals, and bacteria.

Composition of Peroxidase Enzyme

The composition of peroxidase enzyme is as follows:

- Protein - 95%
- Other - 5%
- Sulphur - 5% of 5%

Calculation of Minimum Molecular Weight

To calculate the minimum molecular weight of peroxidase enzyme, we need to know the molecular weight of sulphur. The molecular weight of sulphur is 32.06 g/mol.

Now, we can calculate the minimum molecular weight of peroxidase enzyme as follows:

- Let the minimum molecular weight of peroxidase enzyme be x
- 5% of x = 5% of 5% of x + 95% of x
- 0.05x = 0.0025x + 0.95x
- 0.0975x = x
- x = 32.06 g/mol / 0.0975
- x = 328.21 g/mol

Therefore, the minimum molecular weight of peroxidase enzyme is 328.21 g/mol.

Explanation

The composition of peroxidase enzyme is given as 5% by weight of sulphur. This means that for every 100 grams of peroxidase enzyme, 5 grams of sulphur is present. We need to calculate the minimum molecular weight of the enzyme, which is the lowest possible molecular weight that the enzyme can have while still containing 5% by weight of sulphur.

To calculate the minimum molecular weight, we need to use the formula:

% of sulphur in enzyme = % of sulphur in sulphur / molecular weight of sulphur * 100% + % of other components / molecular weight of other components * 100%

Using this formula, we can solve for the minimum molecular weight of peroxidase enzyme.