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Primers are
- a)Chemically synthesised oligonucleotides that are complementary to the regions of DNA
- b)Chemically synthesised oligonucleotides that are not complementary to the regions of DNA
- c)Chemically synthesised, autonomously replicating circular DNA molecules
- d)Specific sequences present on recombinant DNA
Correct answer is option 'A'. Can you explain this answer?
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Primers area)Chemically synthesised oligonucleotides that are compleme...
Primers are small, chemically synthesised oligonucleotides that are complementary to the sequences, present at 3' end of the template DNA. They hybridise to the target DNA region, one to each strand of the double helix. These primers are oriented with their ends facing each other allowing synthesis of the DNA towards one another.
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Primers area)Chemically synthesised oligonucleotides that are compleme...
Understanding Primers
Primers are essential components in molecular biology, particularly in techniques like Polymerase Chain Reaction (PCR). They enable the amplification of specific DNA sequences. Let’s delve into the details:
Definition of Primers
- Primers are chemically synthesized oligonucleotides.
- They are designed to be complementary to specific regions of a target DNA sequence.
Function of Primers
- Primers anneal to the single-stranded DNA during the PCR process.
- They provide a starting point for DNA polymerase, which extends the primer to replicate the target DNA.
Why Option 'A' is Correct
- Complementarity: The correct primers must match the DNA sequence they are intended to amplify, ensuring specificity in the reaction.
- Role in Amplification: Without complementary primers, the amplification process would not initiate, leading to failure in obtaining the desired DNA fragments.
Why Other Options are Incorrect
- Option B: Non-complementary primers would not anneal to the DNA, making them ineffective for amplification.
- Option C: While circular DNA molecules are important in some contexts, they do not define what primers are.
- Option D: Specific sequences on recombinant DNA do not encapsulate the essential characteristic of primers.
Conclusion
The correct answer, option 'A', accurately captures the essence of what primers are—specifically, they are complementary oligonucleotides crucial for DNA amplification processes. Understanding their role is fundamental for anyone studying molecular biology techniques, especially in the context of NEET and other biological sciences.
Primers are essential components in molecular biology, particularly in techniques like Polymerase Chain Reaction (PCR). They enable the amplification of specific DNA sequences. Let’s delve into the details:
Definition of Primers
- Primers are chemically synthesized oligonucleotides.
- They are designed to be complementary to specific regions of a target DNA sequence.
Function of Primers
- Primers anneal to the single-stranded DNA during the PCR process.
- They provide a starting point for DNA polymerase, which extends the primer to replicate the target DNA.
Why Option 'A' is Correct
- Complementarity: The correct primers must match the DNA sequence they are intended to amplify, ensuring specificity in the reaction.
- Role in Amplification: Without complementary primers, the amplification process would not initiate, leading to failure in obtaining the desired DNA fragments.
Why Other Options are Incorrect
- Option B: Non-complementary primers would not anneal to the DNA, making them ineffective for amplification.
- Option C: While circular DNA molecules are important in some contexts, they do not define what primers are.
- Option D: Specific sequences on recombinant DNA do not encapsulate the essential characteristic of primers.
Conclusion
The correct answer, option 'A', accurately captures the essence of what primers are—specifically, they are complementary oligonucleotides crucial for DNA amplification processes. Understanding their role is fundamental for anyone studying molecular biology techniques, especially in the context of NEET and other biological sciences.
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Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer?
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Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer?.
Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Primers area)Chemically synthesised oligonucleotides that are complementary to the regions of DNAb)Chemically synthesised oligonucleotides that are not complementary to the regions of DNAc)Chemically synthesised, autonomously replicating circular DNA moleculesd)Specific sequences present on recombinant DNACorrect answer is option 'A'. Can you explain this answer?.
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