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Study the following statements regarding recombinant DNA technology and select the incorrect ones
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium
(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture
(vi) Downstream processing is one of the steps of polymerase chain reaction
  • a)
    (i), (iii) and (vi)
  • b)
    (i), (iii) and (v)
  • c)
    (ii), (iii) and (v)
  • d)
    (i), (iv) and (v)
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
Study the following statements regarding recombinant DNA technology an...
Antibiotic resistance genes are selectable markers. Desirable genes are the ones which are introduced in the vector for getting desired protein product. In agarose gel electrophoresis, DNA fragments are separated according to their charge and size. After the formation of the product in a bioreactors, it undergoes through some processes before a finished product is ready for marketing. The processes include separation and purification of products which are collectively called as downstream processing.
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Study the following statements regarding recombinant DNA technology an...
Incorrect statements:
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
(vi) Downstream processing is one of the steps of polymerase chain reaction

Explanation:
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
Taq polymerase is a DNA polymerase enzyme isolated from the bacterium Thermus aquaticus. It is commonly used in polymerase chain reaction (PCR) because of its ability to withstand high temperatures. Taq polymerase does not require nucleotides to extend the primers. It uses the nucleotides provided in the reaction mixture.

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
Agarose gel electrophoresis is a common technique used to separate DNA fragments based on their size, not their charge. Agarose gel is a matrix that forms pores of different sizes, allowing smaller DNA fragments to migrate faster and farther through the gel than larger fragments. The separation is based on the size of the DNA fragments, not their charge.

(vi) Downstream processing is one of the steps of polymerase chain reaction
Downstream processing is not a step of the polymerase chain reaction (PCR). It is a series of steps involved in the purification and isolation of the desired product after the completion of the PCR reaction. Downstream processing includes techniques such as DNA extraction, purification, and analysis.

Correct statement:
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology
Antibiotic resistance genes are often used as selectable markers in recombinant DNA technology. These genes allow researchers to identify and select cells that have taken up the desired recombinant DNA. Cells that have successfully incorporated the antibiotic resistance gene and the recombinant DNA will survive in the presence of the antibiotic, while non-transformed cells will die. This helps in the identification and selection of cells that have successfully incorporated the desired genes.
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Study the following statements regarding recombinant DNA technology an...
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Study the following statements regarding recombinant DNA technology and select the incorrect ones(i) Taq polymerase extends the primers using the nucleotides provided in the reaction(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture(vi) Downstream processing is one of the steps of polymerase chain reactiona)(i), (iii) and (vi)b)(i), (iii) and (v)c)(ii), (iii) and (v)d)(i), (iv) and (v)Correct answer is option 'A'. Can you explain this answer?
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Study the following statements regarding recombinant DNA technology and select the incorrect ones(i) Taq polymerase extends the primers using the nucleotides provided in the reaction(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture(vi) Downstream processing is one of the steps of polymerase chain reactiona)(i), (iii) and (vi)b)(i), (iii) and (v)c)(ii), (iii) and (v)d)(i), (iv) and (v)Correct answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Study the following statements regarding recombinant DNA technology and select the incorrect ones(i) Taq polymerase extends the primers using the nucleotides provided in the reaction(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture(vi) Downstream processing is one of the steps of polymerase chain reactiona)(i), (iii) and (vi)b)(i), (iii) and (v)c)(ii), (iii) and (v)d)(i), (iv) and (v)Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Study the following statements regarding recombinant DNA technology and select the incorrect ones(i) Taq polymerase extends the primers using the nucleotides provided in the reaction(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture(vi) Downstream processing is one of the steps of polymerase chain reactiona)(i), (iii) and (vi)b)(i), (iii) and (v)c)(ii), (iii) and (v)d)(i), (iv) and (v)Correct answer is option 'A'. Can you explain this answer?.
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