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Plasmid used to construct the first recombinant DNA was isolated from which bacterium species?
- a)Escherichia coli
- b)Salmonella typhimurium
- c)Agrobacterium tumefaciens
- d)Thermus aquaticus
Correct answer is option 'B'. Can you explain this answer?
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Plasmid used to construct the first recombinant DNA was isolated from ...
The first recombinant DNA was constructed by Stanley Cohen and Herbert Boyer in 1972. They cut the piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of Escherichia coil.
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Plasmid used to construct the first recombinant DNA was isolated from ...
Isolation of Plasmid for Recombinant DNA
The first recombinant DNA was constructed in 1972, which was a result of the isolation of plasmid from bacteria.
Plasmids are small, circular DNA molecules that can replicate independently from the chromosomal DNA in bacteria. These plasmids carry genes that provide bacteria with additional traits, such as antibiotic resistance.
The plasmid used to construct the first recombinant DNA was isolated from Salmonella typhimurium, a gram-negative bacterium that causes food poisoning in humans and animals.
This plasmid, known as pSC101, was chosen because it was small and easy to manipulate in the laboratory. It contained a gene for ampicillin resistance, which was used as a selectable marker to identify bacteria that had taken up the recombinant DNA.
The construction of the first recombinant DNA involved the insertion of a gene from the frog, Xenopus laevis, into the plasmid. This was done using restriction enzymes, which cut the plasmid and the frog DNA at specific sites, and ligase, which joined the two fragments together.
This recombinant plasmid was then introduced into E. coli, a common bacterium used in genetic engineering, using a process called transformation. The bacteria that took up the recombinant plasmid were able to grow in the presence of ampicillin, which allowed the selection of the transformed cells.
This breakthrough in genetic engineering led to the development of many new techniques and applications, such as the production of human insulin using recombinant DNA technology.
The first recombinant DNA was constructed in 1972, which was a result of the isolation of plasmid from bacteria.
Plasmids are small, circular DNA molecules that can replicate independently from the chromosomal DNA in bacteria. These plasmids carry genes that provide bacteria with additional traits, such as antibiotic resistance.
The plasmid used to construct the first recombinant DNA was isolated from Salmonella typhimurium, a gram-negative bacterium that causes food poisoning in humans and animals.
This plasmid, known as pSC101, was chosen because it was small and easy to manipulate in the laboratory. It contained a gene for ampicillin resistance, which was used as a selectable marker to identify bacteria that had taken up the recombinant DNA.
The construction of the first recombinant DNA involved the insertion of a gene from the frog, Xenopus laevis, into the plasmid. This was done using restriction enzymes, which cut the plasmid and the frog DNA at specific sites, and ligase, which joined the two fragments together.
This recombinant plasmid was then introduced into E. coli, a common bacterium used in genetic engineering, using a process called transformation. The bacteria that took up the recombinant plasmid were able to grow in the presence of ampicillin, which allowed the selection of the transformed cells.
This breakthrough in genetic engineering led to the development of many new techniques and applications, such as the production of human insulin using recombinant DNA technology.
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Plasmid used to construct the first recombinant DNA was isolated from ...
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native *plasmid* (autonomously replicating circular extra-chromosomal DNA) of _Salmonella typhimurium_ .
~ By Stanley Cohen and Herbert Boyer in 1972..
~ By Stanley Cohen and Herbert Boyer in 1972..
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Plasmid used to construct the first recombinant DNA was isolated from which bacterium species?a)Escherichia colib)Salmonella typhimuriumc)Agrobacterium tumefaciensd)Thermus aquaticusCorrect answer is option 'B'. Can you explain this answer?
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