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The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequence of steps?
- a)(i), (iii), (ii), (iv), (v)
- b)(iii), (ii), (i), (v), (iv)
- c)(i), (v), (ii), (iv), (iii)
- d)(v), (i), (iii), (iv), (ii)
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
The different steps of recombinant DNA technology are given below rand...
Understanding Recombinant DNA Technology Steps
Recombinant DNA technology involves several crucial steps to successfully clone and express a gene of interest. The correct sequence of these steps is pivotal for achieving the desired outcome.
1. Isolation of the DNA Fragments
- This is the first step where the specific DNA fragments or genes that need to be cloned are isolated from the source organism.
2. Insertion into a Suitable Plasmid Vector
- The isolated gene is then inserted into a suitable plasmid vector. This vector is essential as it carries the gene into the host cell.
3. Introduction of Recombinant DNA into Host Cells
- The next step involves the introduction of the recombinant DNA (plasmid containing the gene) into a host cell, typically E. coli. This process is known as transformation.
4. Selection of Transformed Host Cells
- After transformation, it is crucial to select the host cells that have successfully taken up the recombinant DNA. Identification techniques are used to isolate those clones containing the desired gene.
5. Multiplication/Expression of the Introduced Gene
- Finally, the transformed cells multiply and express the introduced gene, producing the desired protein or product.
Correct Sequence Summary
- The correct sequence of steps is:
- (i) Isolation of the DNA fragments
- (v) Insertion of the isolated gene in a suitable plasmid vector
- (ii) Introduction of the recombinant DNA into a suitable cell (transformation)
- (iv) Selection of the transformed host cells
- (iii) Multiplication/expression of the introduced gene
This systematic approach ensures that the recombinant DNA technology is executed effectively, leading to successful gene cloning and expression.
Recombinant DNA technology involves several crucial steps to successfully clone and express a gene of interest. The correct sequence of these steps is pivotal for achieving the desired outcome.
1. Isolation of the DNA Fragments
- This is the first step where the specific DNA fragments or genes that need to be cloned are isolated from the source organism.
2. Insertion into a Suitable Plasmid Vector
- The isolated gene is then inserted into a suitable plasmid vector. This vector is essential as it carries the gene into the host cell.
3. Introduction of Recombinant DNA into Host Cells
- The next step involves the introduction of the recombinant DNA (plasmid containing the gene) into a host cell, typically E. coli. This process is known as transformation.
4. Selection of Transformed Host Cells
- After transformation, it is crucial to select the host cells that have successfully taken up the recombinant DNA. Identification techniques are used to isolate those clones containing the desired gene.
5. Multiplication/Expression of the Introduced Gene
- Finally, the transformed cells multiply and express the introduced gene, producing the desired protein or product.
Correct Sequence Summary
- The correct sequence of steps is:
- (i) Isolation of the DNA fragments
- (v) Insertion of the isolated gene in a suitable plasmid vector
- (ii) Introduction of the recombinant DNA into a suitable cell (transformation)
- (iv) Selection of the transformed host cells
- (iii) Multiplication/expression of the introduced gene
This systematic approach ensures that the recombinant DNA technology is executed effectively, leading to successful gene cloning and expression.
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Question Description
The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer? for NEET 2026 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2026 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer?.
The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer? for NEET 2026 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2026 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer?.
Solutions for The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps?a)(i), (iii), (ii), (iv), (v)b)(iii), (ii), (i), (v), (iv)c)(i), (v), (ii), (iv), (iii)d)(v), (i), (iii), (iv), (ii)Correct answer is option 'C'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET. Download more important topics, notes, lectures and mock test series for NEET Exam by signing up for free.
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