Which of the following enzyme types catalyzes the formation of a singl...
Ligases are enzymes that catalyze the formation of a covalent bond between two substrates using ATP or another high-energy molecule as a source of energy. They are involved in the joining of two molecules, typically with the simultaneous release of a small molecule such as water (H2O). This type of reaction is often referred to as a condensation or dehydration reaction, as it involves the elimination of water.
Option A, Isomerase, is an enzyme type that catalyzes the interconversion of isomers, which involves rearranging atoms within a molecule but does not typically involve the formation or breaking of covalent bonds.
Option B, Hydrolase, is an enzyme type that catalyzes the cleavage of a covalent bond by the addition of water (hydrolysis). Hydrolases break down substrates by adding a water molecule, resulting in the formation of two separate molecules.
Option C, Oxidoreductase, is an enzyme type that catalyzes oxidation-reduction reactions. These enzymes are involved in the transfer of electrons between substrates, rather than the formation of single bonds through the elimination of water.
Therefore, the enzyme type that catalyzes the formation of a single bond between two substrates through the elimination of H2O is a Ligase.