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A cell that is too small to be impaled by a microelectrode can be measured and mapped with spatial resolution using
  • a)
    radioisotope
  • b)
    voltage-sensitive dye
  • c)
    pH sensitive chemical
  • d)
    chromophore dyes
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
A cell that is too small to be impaled by a microelectrode can be meas...
Concept:
  • A radioisotope used for diagnosis must emit gamma rays of sufficient energy to escape from the body and it must have a half-life short enough for it to decay away soon after imaging is completed.
  • The radioisotope most widely used in medicine is Tc-99, employed in some 80% of all nuclear medicine procedures.
Explanation:
  • Autoradiography can be used to determine how radioisotopes are distributed spatially within a particular tissue, cell, or molecule.
  • In this technique, proteins that are primarily tagged in vivo with 32P (a high-energy -emitter) or 35S (a low-energy -emitter) are visualised and quantified using X-ray film.
  • The labelling of other radioisotopes, such as 3H, 14C, and 125I, is also an option.
  • In this respect, prior knowledge of the sample's interesting components is necessary for radioactive nuclide labelling. Incorporating such an approach serves as a marker for the proteins found in a culture media.
  • The other option is a direct technique that involves irradiating distinct proteins on a complete 2-DE in order to identify all the elements directly activated by neutron bombardment. 
  • Considering film-based autoradiography, the radioactive atoms will decay in the sample, emitting particles that penetrate the film and eject the electrons from silver halide crystals generating local precipitates of silver atoms.
 
      hence the correct answer is option 1
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A cell that is too small to be impaled by a microelectrode can be measured and mapped with spatial resolution usinga)radioisotopeb)voltage-sensitive dyec)pH sensitive chemicald)chromophore dyesCorrect answer is option 'A'. Can you explain this answer?
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