Rotation at high speed makes the colloid settle down and the impurities remain in solution. What is this process called?a)Ultra centrifugationb)Ultrafiltrationc)Dialysisd)Mechanical dispersionCorrect answer is option 'A'. Can you explain this answer?

Question

Answer

Option(a)becoz--->

High-speed (ultra)centrifugation for fractionation of organelles and molecular components of tissues permits the elucidation of fundamental cellular processes such as
oxidative phosphorylation, intracellular digestion, protein synthesis, bulk transport or biogenesis of organelles.

Answer

HEY USER IN THIS QUESTION WHEN WE ROTATE THE COLLOIDAL SUBSTANCE WITH THE HAI SPEED GENERATE TO A ROTATIONAL MOTION WHERE WE COME TO A V EQUALS TO OMEGA R SO IN THE QUESTION WHEN WE ROTATE THE THE SUBSTANCE IT GETS TO TO CONCENTRATE AT A CENTRIFUGAL FORCE WHERE DOES IT APPEAR THAT'S WHY IN THIS QUESTION WE JUST VERIFY BY THE PROCESS OF CENTRIPETAL FORCE

Answer

Ultra centrifugation
Ultra centrifugation is a process used to separate particles based on their size, shape, and density using high-speed rotation. It is commonly employed in laboratories to separate and purify biological samples, such as proteins, DNA, and viruses.

Principle of Ultra Centrifugation
Ultra centrifugation works on the principle of sedimentation, which is the process by which particles settle down in a liquid medium under the influence of gravitational force. In ultra centrifugation, the gravitational force is increased by spinning the sample at very high speeds, typically in the range of 10,000 to 100,000 revolutions per minute (RPM).

Process of Ultra Centrifugation
The process of ultra centrifugation involves the following steps:

1. Sample Preparation: The sample is prepared by adding a colloidal suspension or a mixture of particles to a suitable liquid medium. The liquid medium is typically a buffer solution that provides the necessary conditions for the particles to remain suspended.

2. Loading the Sample: The prepared sample is loaded into special tubes called centrifuge tubes. These tubes are designed to withstand the high speeds and forces generated during centrifugation.

3. Centrifugation: The loaded tubes are placed in a high-speed centrifuge and spun at the desired speed for a specific duration. As the tubes spin, the centrifugal force causes the particles to sediment at the bottom of the tube.

4. Separation: During centrifugation, the particles in the sample separate into different layers or bands based on their size, shape, and density. The larger and denser particles settle down faster and form a pellet at the bottom of the tube, while the smaller and less dense particles remain in solution.

5. Collection: After centrifugation, the supernatant, which contains the impurities and the particles that did not settle down, is carefully removed without disturbing the pellet. The pellet, which contains the desired particles, is collected and further processed or analyzed.

Advantages of Ultra Centrifugation
- High resolution separation: Ultra centrifugation can separate particles with very similar properties, such as size and density, with high precision.
- Rapid processing: The high speeds and forces involved in ultra centrifugation allow for rapid separation of particles.
- Scalability: Ultra centrifugation can be scaled up or down depending on the sample size and desired separation efficiency.

In conclusion, ultra centrifugation is a powerful technique used for separating particles based on their size, shape, and density. By subjecting the sample to high-speed rotation, the colloid settles down while the impurities remain in solution, allowing for purification and analysis of the desired particles.

Answer

The correct answer is Option A.
Ultracentrifugation involves the separation of colloidal particles from the impurities by centrifugal force.
The impure sol is taken in a tube and the tube is placed in an ultracentrifuge. The tube is rotated at high speeds.
On account of this, the colloidal particles settle down at the bottom of the tube and the impurities remain in the solution.
This solution is termed as centrifugate. The settled colloidal particles are removed from the tube and are mixed with an appropriate dispersing medium. Thus, the pure sol is obtained.

Similar Class 12 Doubts

The process of separation of colloids by passing through semi permeable membrane is called?

What is the difference in a deviation between any two colors called?

What is dispersive power?

Rainbow is formed due to a combination of [2000]

Which method is used for preparation of metal sols?

Top Courses for Class 12

Suggested Free Tests

Related Content

Schools

Competitive Examinations

Quick Access Links

Technical Exams