Culturing Microorganisms | Biology for Grade 10 PDF Download

Binary Fission

• Bacteria multiply by a type of simple cell division known as binary fission
• In the right conditions, a bacterial cell prepares to divide by replicating its genetic material before it increases in size
• A copy of each piece of circular DNA moves to each end of the cell before the cytoplasm divides, and new cell walls form around each daughter cell

Each division of one cell produces two cells, so the number of cells increases by a power of 2 each time binary fission occurs

Growing Bacterial Cultures in the Lab

• The effect of disinfectants and antibiotics on microorganisms can be investigated using cultures of bacteria grown in the lab
• In the right conditions, some species of bacteria (such as coli) can multiply as much as once every 20 minutes. This is ideal as large cultures of bacteria for study can be grown in relatively short periods of time
• To multiply this quickly, bacteria require an adequate supply of nutrients (carbohydrates, proteins, minerals and vitamins) and an appropriate temperature (which varies depending on the species being grown)
  • Warmer temperatures promote faster growth, but in a school lab the maximum allowed temperature for growth is 25°C
  • Above this temperature, more harmful pathogens are likely to grow
• Bacteria can be grown in a nutrient broth solution or as colonies on an agar gel plate

Uncontaminated Cultures & Aseptic Techniques

• It is vital that uncontaminated cultures of microorganisms are grown in the lab
• The presence of competing species can affect the growth of cultures, as well as the validity of any study performed on them
• Some important aseptic techniques are outlined in the table below:

Uncontaminated culture preparation table

Calculating Inhibition Zone Area

• The effectiveness of different antibiotics, antiseptics or disinfectants can be determined by calculating the area of an inhibition zone around a disc of the substance being tested
• To calculate the area of an inhibition zone you should use the equation:

Calculating area

Example:

Calculating the area of a clear zone is a far more accurate way of comparing the effect of different substances on bacterial growth than trying to judge by sight

Calculating Bacteria in a Population

• The average amount of time it takes for a bacterial cell in a population to divide is the mean division time
• The number of times a cell has divided and how many cells it produces can be determined if you know the mean division time and how long division has been occurring

Example: If a bacterial cell has a mean division time of 24 minutes and has been dividing for 4 hours. how many cells will it have produced? 

Calculations in Standard Form

• If you are calculating the number of bacteria present in a population, you are likely to be handling very large numbers (as bacteria can multiply very quickly in certain conditions)
• You should be able to express an answer for the number of bacteria in standard form

Example: A bacterial cell has a mean division time of 1260 seconds and has been dividing for 6 hours. Calculate how many bacterial cells it would have produced. Give your answer in standard form.